Solution Structure of the DNA complex of the C-terminal Domain of Rok
GTTRRRRGTA RPGSKAAKLR EAAIKTLKRH NAAIKSSELQ KEIEKESGLE IPNMTTFMQS LIKMYPEVKK PYRGQYILEG EIESLEHHHH HH
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.6 % (1063 of 1287) | 83.2 % (633 of 761) | 81.2 % (345 of 425) | 84.2 % (85 of 101) |
Backbone | 87.5 % (586 of 670) | 91.3 % (285 of 312) | 81.9 % (221 of 270) | 90.9 % (80 of 88) |
Sidechain | 78.8 % (554 of 703) | 77.5 % (348 of 449) | 83.4 % (201 of 241) | 38.5 % (5 of 13) |
Aromatic | 41.8 % (41 of 98) | 55.2 % (37 of 67) | 12.9 % (4 of 31) | |
Methyl | 98.9 % (92 of 93) | 98.0 % (49 of 50) | 100.0 % (43 of 43) |
1. entity 1
GTTRRRRGTA RPGSKAAKLR EAAIKTLKRH NAAIKSSELQ KEIEKESGLE IPNMTTFMQS LIKMYPEVKK PYRGQYILEG EIESLEHHHH HH2. entity 2
CTAATAACTA GTTATTAGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 0.5 mM [U-99% 13C; U-99% 15N] Rok, 2.5 mM 1H_12C_14N Seq1, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Rok | [U-99% 13C; U-99% 15N] | protein | 0.5 mM |
2 | Seq1 | [U-13C;U-15N] | DNA | 2.5 mM |
3 | H2O | natural abundance | solvent | 90 % |
4 | D2O | [U-2H] | solvent | 10 % |