BMRBj - BMREntryMaster

Found 1 Document (1.616 seconds)

BMRB: 36294

6L8V

membrane-bound Bax helix2-helix5 domain

Authors

Bo, O.Y., Fujiao, L.

Assembly

Apoptosis regulator BAX

Entity

1. Apoptosis regulator BAX (polymer, Thiol state: not present), 81 monomers, 8979.171 × 2 Da Detail

GPGSMDASTK KLSESLKRIG DELDSNMELQ RMIAAVDTDS PREVFFRVAA DMFSDGNFNW GRVVALFYFA SKLVLKALST K

Total weight

17958.342 Da

Max. entity weight

8979.171 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 77, ¹³C: 233, ¹⁵N: 77 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	2	PRO	C	C	176.96	0.3	1
2	1	2	PRO	CA	C	62.77	0.3	1
3	1	2	PRO	CB	C	31.02	0.3	1
4	1	3	GLY	H	H	8.59	0.03	1
5	1	3	GLY	C	C	173.96	0.3	1
6	1	3	GLY	CA	C	44.49	0.3	1
7	1	3	GLY	N	N	110.48	0.3	1
8	1	4	SER	H	H	8.15	0.03	1
9	1	4	SER	C	C	174.54	0.3	1
10	1	4	SER	CA	C	57.89	0.3	1
11	1	4	SER	CB	C	62.65	0.3	1
12	1	4	SER	N	N	116.37	0.3	1
13	1	5	MET	H	H	8.44	0.03	1
14	1	5	MET	C	C	176.03	0.3	1
15	1	5	MET	CA	C	55.25	0.3	1
16	1	5	MET	CB	C	31.90	0.3	1
17	1	5	MET	N	N	122.90	0.3	1
18	1	6	ASP	H	H	8.24	0.03	1
19	1	6	ASP	C	C	176.03	0.3	1
20	1	6	ASP	CA	C	54.34	0.3	1
21	1	6	ASP	CB	C	40.39	0.3	1
22	1	6	ASP	N	N	122.58	0.3	1
23	1	7	ALA	H	H	8.31	0.03	1
24	1	7	ALA	C	C	179.58	0.3	1
25	1	7	ALA	CA	C	53.83	0.3	1
26	1	7	ALA	CB	C	17.55	0.3	1
27	1	7	ALA	N	N	123.86	0.3	1
28	1	8	SER	H	H	8.36	0.03	1
29	1	8	SER	C	C	176.42	0.3	1
30	1	8	SER	CA	C	60.43	0.3	1
31	1	8	SER	CB	C	61.96	0.3	1
32	1	8	SER	N	N	115.30	0.3	1
33	1	9	THR	H	H	8.15	0.03	1
34	1	9	THR	C	C	175.39	0.3	1
35	1	9	THR	CA	C	64.90	0.3	1
36	1	9	THR	CB	C	67.06	0.3	1
37	1	9	THR	N	N	118.41	0.3	1
38	1	10	LYS	H	H	7.86	0.03	1
39	1	10	LYS	C	C	177.90	0.3	1
40	1	10	LYS	CA	C	58.10	0.3	1
41	1	10	LYS	CB	C	30.96	0.3	1
42	1	10	LYS	N	N	123.65	0.3	1
43	1	11	LYS	H	H	7.82	0.03	1
44	1	11	LYS	C	C	179.17	0.3	1
45	1	11	LYS	CA	C	58.44	0.3	1
46	1	11	LYS	CB	C	31.03	0.3	1
47	1	11	LYS	N	N	119.15	0.3	1
48	1	12	LEU	H	H	7.62	0.03	1
49	1	12	LEU	C	C	177.47	0.3	1
50	1	12	LEU	CA	C	57.28	0.3	1
51	1	12	LEU	CB	C	40.08	0.3	1
52	1	12	LEU	N	N	122.58	0.3	1
53	1	13	SER	H	H	8.40	0.03	1
54	1	13	SER	C	C	176.28	0.3	1
55	1	13	SER	CA	C	61.03	0.3	1
56	1	13	SER	CB	C	62.50	0.3	1
57	1	13	SER	N	N	115.30	0.3	1
58	1	14	GLU	H	H	8.19	0.03	1
59	1	14	GLU	C	C	178.96	0.3	1
60	1	14	GLU	CA	C	58.34	0.3	1
61	1	14	GLU	CB	C	28.34	0.3	1
62	1	14	GLU	N	N	119.48	0.3	1
63	1	15	SER	H	H	7.87	0.03	1
64	1	15	SER	C	C	177.24	0.3	1
65	1	15	SER	CA	C	61.63	0.3	1
66	1	15	SER	CB	C	60.71	0.3	1
67	1	15	SER	N	N	116.37	0.3	1
68	1	16	LEU	H	H	8.31	0.03	1
69	1	16	LEU	C	C	178.45	0.3	1
70	1	16	LEU	CA	C	57.22	0.3	1
71	1	16	LEU	CB	C	40.53	0.3	1
72	1	16	LEU	N	N	121.30	0.3	1
73	1	17	LYS	H	H	8.15	0.03	1
74	1	17	LYS	C	C	179.39	0.3	1
75	1	17	LYS	CA	C	57.74	0.3	1
76	1	17	LYS	CB	C	28.72	0.3	1
77	1	17	LYS	N	N	116.37	0.3	1
78	1	18	ARG	H	H	8.27	0.03	1
79	1	18	ARG	C	C	178.92	0.3	1
80	1	18	ARG	CA	C	58.60	0.3	1
81	1	18	ARG	CB	C	28.68	0.3	1
82	1	18	ARG	N	N	120.44	0.3	1
83	1	19	ILE	H	H	7.98	0.03	1
84	1	19	ILE	C	C	178.07	0.3	1
85	1	19	ILE	CA	C	63.99	0.3	1
86	1	19	ILE	CB	C	37.37	0.3	1
87	1	19	ILE	N	N	119.69	0.3	1
88	1	20	GLY	H	H	8.19	0.03	1
89	1	20	GLY	C	C	174.23	0.3	1
90	1	20	GLY	CA	C	46.83	0.3	1
91	1	20	GLY	N	N	106.74	0.3	1
92	1	21	ASP	H	H	8.79	0.03	1
93	1	21	ASP	C	C	179.46	0.3	1
94	1	21	ASP	CA	C	56.57	0.3	1
95	1	21	ASP	CB	C	38.06	0.3	1
96	1	21	ASP	N	N	123.44	0.3	1
97	1	22	GLU	H	H	8.47	0.03	1
98	1	22	GLU	C	C	178.76	0.3	1
99	1	22	GLU	CA	C	58.36	0.3	1
100	1	22	GLU	CB	C	28.12	0.3	1
101	1	22	GLU	N	N	122.90	0.3	1
102	1	23	LEU	H	H	7.63	0.03	1
103	1	23	LEU	C	C	177.75	0.3	1
104	1	23	LEU	CA	C	57.49	0.3	1
105	1	23	LEU	CB	C	40.45	0.3	1
106	1	23	LEU	N	N	121.40	0.3	1
107	1	24	ASP	H	H	7.61	0.03	1
108	1	24	ASP	C	C	176.26	0.3	1
109	1	24	ASP	CA	C	55.66	0.3	1
110	1	24	ASP	CB	C	41.72	0.3	1
111	1	24	ASP	N	N	115.84	0.3	1
112	1	25	SER	H	H	7.72	0.03	1
113	1	25	SER	C	C	173.48	0.3	1
114	1	25	SER	CA	C	57.38	0.3	1
115	1	25	SER	CB	C	63.40	0.3	1
116	1	25	SER	N	N	110.38	0.3	1
117	1	26	ASN	H	H	7.25	0.03	1
118	1	26	ASN	C	C	175.32	0.3	1
119	1	26	ASN	CA	C	53.22	0.3	1
120	1	26	ASN	CB	C	36.88	0.3	1
121	1	26	ASN	N	N	121.62	0.3	1
122	1	27	MET	H	H	8.72	0.03	1
123	1	27	MET	C	C	177.96	0.3	1
124	1	27	MET	CA	C	57.08	0.3	1
125	1	27	MET	CB	C	30.81	0.3	1
126	1	27	MET	N	N	129.22	0.3	1
127	1	28	GLU	H	H	8.32	0.03	1
128	1	28	GLU	C	C	178.41	0.3	1
129	1	28	GLU	CA	C	58.60	0.3	1
130	1	28	GLU	CB	C	28.12	0.3	1
131	1	28	GLU	N	N	122.04	0.3	1
132	1	29	LEU	H	H	7.97	0.03	1
133	1	29	LEU	C	C	178.21	0.3	1
134	1	29	LEU	CA	C	56.98	0.3	1
135	1	29	LEU	CB	C	40.08	0.3	1
136	1	29	LEU	N	N	121.40	0.3	1
137	1	30	GLN	H	H	8.34	0.03	1
138	1	30	GLN	C	C	176.54	0.3	1
139	1	30	GLN	CA	C	59.72	0.3	1
140	1	30	GLN	CB	C	31.61	0.3	1
141	1	30	GLN	N	N	119.80	0.3	1
142	1	31	ARG	H	H	7.61	0.03	1
143	1	31	ARG	C	C	178.57	0.3	1
144	1	31	ARG	CA	C	58.70	0.3	1
145	1	31	ARG	CB	C	28.42	0.3	1
146	1	31	ARG	N	N	118.51	0.3	1
147	1	32	MET	H	H	7.95	0.03	1
148	1	32	MET	C	C	178.64	0.3	1
149	1	32	MET	CA	C	58.81	0.3	1
150	1	32	MET	CB	C	33.36	0.3	1
151	1	32	MET	N	N	119.65	0.3	1
152	1	33	ILE	H	H	8.23	0.03	1
153	1	33	ILE	C	C	177.39	0.3	1
154	1	33	ILE	CA	C	65.10	0.3	1
155	1	33	ILE	CB	C	36.72	0.3	1
156	1	33	ILE	N	N	119.90	0.3	1
157	1	34	ALA	H	H	8.00	0.03	1
158	1	34	ALA	C	C	177.94	0.3	1
159	1	34	ALA	CA	C	53.52	0.3	1
160	1	34	ALA	CB	C	17.15	0.3	1
161	1	34	ALA	N	N	121.40	0.3	1
162	1	35	ALA	H	H	7.36	0.03	1
163	1	35	ALA	C	C	177.71	0.3	1
164	1	35	ALA	CA	C	52.00	0.3	1
165	1	35	ALA	CB	C	17.97	0.3	1
166	1	35	ALA	N	N	118.30	0.3	1
167	1	36	VAL	H	H	7.20	0.03	1
168	1	36	VAL	C	C	175.05	0.3	1
169	1	36	VAL	CA	C	61.75	0.3	1
170	1	36	VAL	CB	C	30.73	0.3	1
171	1	36	VAL	N	N	116.26	0.3	1
172	1	37	ASP	H	H	8.01	0.03	1
173	1	37	ASP	C	C	176.14	0.3	1
174	1	37	ASP	CA	C	54.13	0.3	1
175	1	37	ASP	CB	C	40.68	0.3	1
176	1	37	ASP	N	N	124.94	0.3	1
177	1	38	THR	H	H	8.03	0.03	1
178	1	38	THR	C	C	174.11	0.3	1
179	1	38	THR	CA	C	61.45	0.3	1
180	1	38	THR	CB	C	68.55	0.3	1
181	1	38	THR	N	N	115.19	0.3	1
182	1	39	ASP	H	H	8.18	0.03	1
183	1	39	ASP	C	C	175.99	0.3	1
184	1	39	ASP	CA	C	54.84	0.3	1
185	1	39	ASP	CB	C	40.39	0.3	1
186	1	39	ASP	N	N	122.26	0.3	1
187	1	40	SER	H	H	8.19	0.03	1
188	1	40	SER	C	C	172.20	0.3	1
189	1	40	SER	CA	C	54.62	0.3	1
190	1	40	SER	CB	C	62.65	0.3	1
191	1	40	SER	N	N	114.55	0.3	1
192	1	41	PRO	C	C	177.35	0.3	1
193	1	41	PRO	CA	C	64.50	0.3	1
194	1	41	PRO	CB	C	30.73	0.3	1
195	1	42	ARG	H	H	8.45	0.03	1
196	1	42	ARG	C	C	176.85	0.3	1
197	1	42	ARG	CA	C	59.52	0.3	1
198	1	42	ARG	CB	C	28.27	0.3	1
199	1	42	ARG	N	N	118.41	0.3	1
200	1	43	GLU	H	H	7.82	0.03	1
201	1	43	GLU	C	C	179.15	0.3	1
202	1	43	GLU	CA	C	58.08	0.3	1
203	1	43	GLU	CB	C	28.19	0.3	1
204	1	43	GLU	N	N	119.15	0.3	1
205	1	44	VAL	H	H	7.82	0.03	1
206	1	44	VAL	C	C	176.93	0.3	1
207	1	44	VAL	CA	C	65.91	0.3	1
208	1	44	VAL	CB	C	31.02	0.3	1
209	1	44	VAL	N	N	119.15	0.3	1
210	1	45	PHE	H	H	8.46	0.03	1
211	1	45	PHE	C	C	175.95	0.3	1
212	1	45	PHE	CA	C	62.16	0.3	1
213	1	45	PHE	CB	C	38.05	0.3	1
214	1	45	PHE	N	N	119.90	0.3	1
215	1	46	PHE	H	H	8.26	0.03	1
216	1	46	PHE	C	C	177.55	0.3	1
217	1	46	PHE	CA	C	60.84	0.3	1
218	1	46	PHE	CB	C	37.37	0.3	1
219	1	46	PHE	N	N	116.16	0.3	1
220	1	47	ARG	H	H	8.13	0.03	1
221	1	47	ARG	C	C	178.64	0.3	1
222	1	47	ARG	CA	C	58.70	0.3	1
223	1	47	ARG	CB	C	28.44	0.3	1
224	1	47	ARG	N	N	120.76	0.3	1
225	1	48	VAL	H	H	8.34	0.03	1
226	1	48	VAL	C	C	177.15	0.3	1
227	1	48	VAL	CA	C	65.31	0.3	1
228	1	48	VAL	CB	C	30.63	0.3	1
229	1	48	VAL	N	N	120.97	0.3	1
230	1	49	ALA	H	H	8.42	0.03	1
231	1	49	ALA	C	C	178.10	0.3	1
232	1	49	ALA	CA	C	54.54	0.3	1
233	1	49	ALA	CB	C	17.58	0.3	1
234	1	49	ALA	N	N	121.08	0.3	1
235	1	50	ALA	H	H	8.02	0.03	1
236	1	50	ALA	C	C	180.17	0.3	1
237	1	50	ALA	CA	C	54.34	0.3	1
238	1	50	ALA	CB	C	17.13	0.3	1
239	1	50	ALA	N	N	117.23	0.3	1
240	1	51	ASP	H	H	7.73	0.03	1
241	1	51	ASP	C	C	179.00	0.3	1
242	1	51	ASP	CA	C	56.57	0.3	1
243	1	51	ASP	CB	C	40.39	0.3	1
244	1	51	ASP	N	N	118.08	0.3	1
245	1	52	MET	H	H	8.39	0.03	1
246	1	52	MET	C	C	175.83	0.3	1
247	1	52	MET	CA	C	58.10	0.3	1
248	1	52	MET	CB	C	31.90	0.3	1
249	1	52	MET	N	N	119.58	0.3	1
250	1	53	PHE	H	H	7.20	0.03	1
251	1	53	PHE	C	C	176.57	0.3	1
252	1	53	PHE	CA	C	56.88	0.3	1
253	1	53	PHE	CB	C	38.05	0.3	1
254	1	53	PHE	N	N	112.52	0.3	1
255	1	54	SER	H	H	7.62	0.03	1
256	1	54	SER	C	C	174.04	0.3	1
257	1	54	SER	CA	C	60.43	0.3	1
258	1	54	SER	N	N	117.87	0.3	1
259	1	55	ASP	H	H	8.08	0.03	1
260	1	55	ASP	C	C	176.85	0.3	1
261	1	55	ASP	CA	C	52.81	0.3	1
262	1	55	ASP	CB	C	39.22	0.3	1
263	1	55	ASP	N	N	120.65	0.3	1
264	1	56	GLY	H	H	8.09	0.03	1
265	1	56	GLY	C	C	172.90	0.3	1
266	1	56	GLY	CA	C	45.08	0.3	1
267	1	56	GLY	N	N	108.02	0.3	1
268	1	57	ASN	H	H	7.96	0.03	1
269	1	57	ASN	C	C	172.99	0.3	1
270	1	57	ASN	CA	C	51.49	0.3	1
271	1	57	ASN	CB	C	38.01	0.3	1
272	1	57	ASN	N	N	119.69	0.3	1
273	1	58	PHE	H	H	8.33	0.03	1
274	1	58	PHE	C	C	173.72	0.3	1
275	1	58	PHE	CA	C	57.59	0.3	1
276	1	58	PHE	CB	C	39.28	0.3	1
277	1	58	PHE	N	N	120.97	0.3	1
278	1	59	ASN	H	H	6.59	0.03	1
279	1	59	ASN	C	C	174.82	0.3	1
280	1	59	ASN	CA	C	52.20	0.3	1
281	1	59	ASN	CB	C	39.03	0.3	1
282	1	59	ASN	N	N	116.80	0.3	1
283	1	60	TRP	H	H	9.26	0.03	1
284	1	60	TRP	C	C	175.55	0.3	1
285	1	60	TRP	CA	C	59.71	0.3	1
286	1	60	TRP	CB	C	28.94	0.3	1
287	1	60	TRP	N	N	120.65	0.3	1
288	1	61	GLY	H	H	9.40	0.03	1
289	1	61	GLY	C	C	176.10	0.3	1
290	1	61	GLY	CA	C	46.95	0.3	1
291	1	61	GLY	N	N	111.88	0.3	1
292	1	62	ARG	H	H	7.93	0.03	1
293	1	62	ARG	C	C	175.68	0.3	1
294	1	62	ARG	CA	C	57.85	0.3	1
295	1	62	ARG	CB	C	30.21	0.3	1
296	1	62	ARG	N	N	120.44	0.3	1
297	1	63	VAL	H	H	7.17	0.03	1
298	1	63	VAL	C	C	174.47	0.3	1
299	1	63	VAL	CA	C	66.63	0.3	1
300	1	63	VAL	N	N	117.79	0.3	1
301	1	64	VAL	C	C	177.24	0.3	1
302	1	64	VAL	CA	C	65.86	0.3	1
303	1	65	ALA	H	H	8.26	0.03	1
304	1	65	ALA	C	C	178.78	0.3	1
305	1	65	ALA	CA	C	55.18	0.3	1
306	1	65	ALA	CB	C	17.28	0.3	1
307	1	65	ALA	N	N	124.83	0.3	1
308	1	66	LEU	H	H	8.27	0.03	1
309	1	66	LEU	C	C	178.60	0.3	1
310	1	66	LEU	CA	C	57.28	0.3	1
311	1	66	LEU	CB	C	40.28	0.3	1
312	1	66	LEU	N	N	119.15	0.3	1
313	1	67	PHE	H	H	8.88	0.03	1
314	1	67	PHE	C	C	177.55	0.3	1
315	1	67	PHE	CA	C	59.72	0.3	1
316	1	67	PHE	CB	C	37.55	0.3	1
317	1	67	PHE	N	N	120.97	0.3	1
318	1	68	TYR	H	H	8.94	0.03	1
319	1	68	TYR	C	C	176.57	0.3	1
320	1	68	TYR	CA	C	61.87	0.3	1
321	1	68	TYR	CB	C	37.01	0.3	1
322	1	68	TYR	N	N	123.12	0.3	1
323	1	69	PHE	H	H	8.21	0.03	1
324	1	69	PHE	C	C	175.48	0.3	1
325	1	69	PHE	CA	C	58.62	0.3	1
326	1	69	PHE	CB	C	38.74	0.3	1
327	1	69	PHE	N	N	119.48	0.3	1
328	1	70	ALA	H	H	8.95	0.03	1
329	1	70	ALA	C	C	178.25	0.3	1
330	1	70	ALA	CA	C	54.24	0.3	1
331	1	70	ALA	CB	C	17.33	0.3	1
332	1	70	ALA	N	N	120.01	0.3	1
333	1	71	SER	H	H	8.05	0.03	1
334	1	71	SER	C	C	174.63	0.3	1
335	1	71	SER	CA	C	61.55	0.3	1
336	1	71	SER	CB	C	63.69	0.3	1
337	1	71	SER	N	N	113.16	0.3	1
338	1	72	LYS	H	H	7.22	0.03	1
339	1	72	LYS	C	C	179.27	0.3	1
340	1	72	LYS	CA	C	56.67	0.3	1
341	1	72	LYS	CB	C	29.85	0.3	1
342	1	72	LYS	N	N	120.65	0.3	1
343	1	73	LEU	H	H	7.85	0.03	1
344	1	73	LEU	C	C	178.84	0.3	1
345	1	73	LEU	CA	C	56.77	0.3	1
346	1	73	LEU	CB	C	40.69	0.3	1
347	1	73	LEU	N	N	120.97	0.3	1
348	1	74	VAL	H	H	7.96	0.03	1
349	1	74	VAL	C	C	176.77	0.3	1
350	1	74	VAL	CA	C	65.61	0.3	1
351	1	74	VAL	CB	C	30.17	0.3	1
352	1	74	VAL	N	N	120.12	0.3	1
353	1	75	LEU	H	H	7.76	0.03	1
354	1	75	LEU	C	C	179.27	0.3	1
355	1	75	LEU	CA	C	57.28	0.3	1
356	1	75	LEU	CB	C	39.92	0.3	1
357	1	75	LEU	N	N	119.05	0.3	1
358	1	76	LYS	H	H	7.65	0.03	1
359	1	76	LYS	C	C	178.57	0.3	1
360	1	76	LYS	CA	C	57.89	0.3	1
361	1	76	LYS	CB	C	30.90	0.3	1
362	1	76	LYS	N	N	119.90	0.3	1
363	1	77	ALA	H	H	7.91	0.03	1
364	1	77	ALA	C	C	178.84	0.3	1
365	1	77	ALA	CA	C	53.73	0.3	1
366	1	77	ALA	CB	C	17.26	0.3	1
367	1	77	ALA	N	N	122.69	0.3	1
368	1	78	LEU	H	H	7.84	0.03	1
369	1	78	LEU	C	C	177.04	0.3	1
370	1	78	LEU	CA	C	54.95	0.3	1
371	1	78	LEU	CB	C	40.74	0.3	1
372	1	78	LEU	N	N	116.37	0.3	1
373	1	79	SER	H	H	7.60	0.03	1
374	1	79	SER	C	C	174.27	0.3	1
375	1	79	SER	CA	C	58.40	0.3	1
376	1	79	SER	CB	C	63.23	0.3	1
377	1	79	SER	N	N	114.12	0.3	1
378	1	80	THR	H	H	7.88	0.03	1
379	1	80	THR	C	C	174.19	0.3	1
380	1	80	THR	CA	C	61.55	0.3	1
381	1	80	THR	CB	C	68.79	0.3	1
382	1	80	THR	N	N	116.37	0.3	1
383	1	81	LYS	H	H	8.22	0.03	1
384	1	81	LYS	C	C	176.50	0.3	1
385	1	81	LYS	CA	C	55.76	0.3	1
386	1	81	LYS	CB	C	31.91	0.3	1
387	1	81	LYS	N	N	124.72	0.3	1

Protein Blocks Logo

Calculated from 15 models in PDB: 6L8V, Strand ID: A, B Detail

Release date

2020-09-22

Citation

An amphipathic Bax core dimer forms part of the apoptotic pore wall in the mitochondrial␣membrane
Lv, F., Qi, F., Zhang, Z., Wen, M., Kale, J., Piai, A., Du, L., Wang, S., Zhou, L., Yang, Y., Wu, B., Liu, Z., Del Rosario, J., Pogmore, J., Chou, J.J., Andrews, D.W., Lin, J., OuYang, B.
EMBO J. (2021), 40, e106438-e106438, PubMed 34101209 , DOI 10.15252/embj.2020106438 , Abstract

Related entities 1. Apoptosis regulator BAX, : 1 : 28 : 125 entities Detail

Interaction partners 1. Apoptosis regulator BAX, : 59 interactors Detail

More details for 59 interactors identified by 39 citations

Experiments performed 15 experiments Detail

1 2D TROSY-HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.0, Details 0.5 mM U-98% 15N;U-98% 13C;U-98% 2H human Bax core domain, 100 mM DMPC, 140 mM DHPC, 10 mM MES, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

2 TROSY-HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

3 TROSY-HNCOCA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

4 TROSY-HNCACO

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

5 TROSY-HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

6 3D 15N NOE-TROSY-HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

7 3D 15N NOE-TROSY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.0, Details 0.6 mM U-98% 15N;U-98% 13C Bax a_2_a_5, 100 mM [U-2H] DMPC, 160 mM [U-2H] DHPC, 10 mM MES, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	Bax a_2_a_5	[U-98% 15N; U-98% 13C]	protein	0.6 mM
9	DHPC	[U-2H]		160 mM
10	DMPC	[U-2H]		100 mM
11	MES	natural abundance	buffer	10 mM
12	sodium chloride	natural abundance	salt	100 mM
13	H2O	natural abundance	solvent	90 %
14	D2O	[U-2H]	solvent	10 %

8 3D 13C NOE-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
8	Bax a_2_a_5	[U-98% 15N; U-98% 13C]	protein	0.6 mM
9	DHPC	[U-2H]		160 mM
10	DMPC	[U-2H]		100 mM
11	MES	natural abundance	buffer	10 mM
12	sodium chloride	natural abundance	salt	100 mM
13	H2O	natural abundance	solvent	90 %
14	D2O	[U-2H]	solvent	10 %

9 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
8	Bax a_2_a_5	[U-98% 15N; U-98% 13C]	protein	0.6 mM
9	DHPC	[U-2H]		160 mM
10	DMPC	[U-2H]		100 mM
11	MES	natural abundance	buffer	10 mM
12	sodium chloride	natural abundance	salt	100 mM
13	H2O	natural abundance	solvent	90 %
14	D2O	[U-2H]	solvent	10 %

10 3D 15N NOE-TROSY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.0, Details 0.4 mM U-98% 15N;U-2H Bax a_2_a_5, 120 mM [U-2H] DMPC, 200 mM [U-2H] DHPC, 10 mM MES, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
15	Bax a_2_a_5	U-98% 15N;U-2H		0.4 mM
16	DHPC	[U-2H]		200 mM
17	DMPC	[U-2H]		120 mM
18	MES	natural abundance		10 mM
19	sodium chloride	natural abundance		100 mM
20	H2O	natural abundance	solvent	90 %
21	D2O	[U-2H]	solvent	10 %

11 2D TROSY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
15	Bax a_2_a_5	U-98% 15N;U-2H		0.4 mM
16	DHPC	[U-2H]		200 mM
17	DMPC	[U-2H]		120 mM
18	MES	natural abundance		10 mM
19	sodium chloride	natural abundance		100 mM
20	H2O	natural abundance	solvent	90 %
21	D2O	[U-2H]	solvent	10 %

12 3D 15N NOE-TROSY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.0, Details 0.6 mM U-98% 15N;U-2H Bax a_2_a_5, 100 mM DMPC, 160 mM [U-2H] DHPC, 10 mM MES, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
22	Bax a_2_a_5	[U-98% 15N; U-2H]	protein	0.6 mM
23	DHPC	[U-2H]		160 mM
24	DMPC	natural abundance		100 mM
25	MES	natural abundance	buffer	10 mM
26	sodium chloride	natural abundance	salt	100 mM
27	H2O	natural abundance	solvent	90 %
28	D2O	[U-2H]	solvent	10 %

13 2D TROSY-HSQC

Instrument

Bruker AVANCE - 900 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
22	Bax a_2_a_5	[U-98% 15N; U-2H]	protein	0.6 mM
23	DHPC	[U-2H]		160 mM
24	DMPC	natural abundance		100 mM
25	MES	natural abundance	buffer	10 mM
26	sodium chloride	natural abundance	salt	100 mM
27	H2O	natural abundance	solvent	90 %
28	D2O	[U-2H]	solvent	10 %

14 2D 1H-13C HSQC

Instrument

Agilent DD2 - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.0, Details 0.4 mM U-98% 15N;U-15% 13C Bax a_2_a_5, 80 mM DMPC, 140 mM DHPC, 10 mM MES, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
29	Bax a_2_a_5	[U-98% 15N; U-15% 13C]	protein	0.4 mM
30	DHPC	natural abundance		140 mM
31	DMPC	natural abundance		80 mM
32	MES	natural abundance	buffer	10 mM
33	sodium chloride	natural abundance	salt	100 mM
34	H2O	natural abundance	solvent	90 %
35	D2O	[U-2H]	solvent	10 %

15 TROSY-HNCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Bax core domain	[U-98% 15N; U-98% 13C; U-98% 2H]	protein	0.5 mM
2	DHPC	natural abundance		140 mM
3	DMPC	natural abundance		100 mM
4	MES	natural abundance	buffer	10 mM
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36294_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36294_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	387		98.8 (chain: 1, length: 81)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 81, Sequence coverage: 98.8%
- Total number of assignments
  - ¹³C chemical shifts: 233
  - ¹H chemical shifts: 77
  - ¹⁵N chemical shifts: 77
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	367	233	63.5
¹H chemical shifts	484	77	15.9
¹⁵N chemical shifts	89	77	86.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	162	160	98.8
¹H chemical shifts	165	77	46.7
¹⁵N chemical shifts	79	77	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	205	73	35.6
¹H chemical shifts	319	0	0.0
¹⁵N chemical shifts	10	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	47	8	17.0
¹H chemical shifts	47	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	39	0	0.0
¹H chemical shifts	40	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords APOPTOSIS, Bax, core-domain, dimer, membrane-bound, stability

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Found 1 Document (1.616 seconds)

BMRB: 36294

Related entities 1. Apoptosis regulator BAX, : 1 : 28 : 125 entities Detail

Interaction partners 1. Apoptosis regulator BAX, : 59 interactors Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 3 contents Detail