BMRBj - BMREntryMaster

Found 1 Document (3.01 seconds)

BMRB: 36309

6LQZ

Solution structure of Taf14ET-Sth1EBMC

Authors

Wu, B., Chen, G., Chen, Y.

Assembly

Transcription initiation factor TFIID subunit 14, Nuclear protein STH1/NPS1 (E.C.3.6.4.12)

Entity

1. Transcription initiation factor TFIID subunit 14 (polymer, Thiol state: not present), 72 monomers, 8076.029 Da Detail

SKGSVDLEKL AFGLTKLNED DLVGVVQMVT DNKTPEMNVT NNVEEGEFII DLYSLPEGLL KSLWDYVKKN TE

2. Nuclear protein STH1/NPS1 (polymer, Thiol state: not present), 60 monomers, 6601.510 Da Detail

SEVKSSSVEI INGSESKKKK PKLTVKIKLN KTTVLENNDG KRAEEKPESK SPAKKTAAKY

Total weight

14677.539 Da

Max. entity weight

8076.029 Da

Source organism

Saccharomyces cerevisiae S288C

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 88.9 %, Completeness (bb): 90.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.9 % (1390 of 1563)	89.1 % (732 of 822)	89.0 % (535 of 601)	87.9 % (123 of 140)
Backbone	90.4 % (707 of 782)	89.5 % (238 of 266)	92.0 % (358 of 389)	87.4 % (111 of 127)
Sidechain	88.3 % (800 of 906)	88.8 % (494 of 556)	87.2 % (294 of 337)	92.3 % (12 of 13)
Aromatic	58.9 % (33 of 56)	71.4 % (20 of 28)	44.4 % (12 of 27)	100.0 % (1 of 1)
Methyl	95.3 % (141 of 148)	97.3 % (72 of 74)	93.2 % (69 of 74)

1. Transcription initiation factor TFIID subunit 14

SKGSVDLEKL AFGLTKLNED DLVGVVQMVT DNKTPEMNVT NNVEEGEFII DLYSLPEGLL KSLWDYVKKN TE

2. Nuclear protein STH1/NPS1

SEVKSSSVEI INGSESKKKK PKLTVKIKLN KTTVLENNDG KRAEEKPESK SPAKKTAAKY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] Taf14ET, 1.5 mM [U-100% 13C; U-100% 15N] Sth1EBMC, 20 mM Phosphate buffer, 100 mM sodium chloride, 1 % v/v inhabitor cocktail, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 6LQZ, Strand ID: A, B Detail

Release date

2020-08-23

Citation

Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation
Chen, G., Wang, D., Wu, B., Yan, F., Xue, H., Wang, Q., Quan, S., Chen, Y.
Nat. Commun. (2020), 11, 4206-4206, PubMed 32826896 , DOI 10.1038/s41467-020-18021-7 , Abstract

Related entities 1. Transcription initiation factor TFIID subunit 14, : 1 : 3 entities Detail

Related entities 2. Nuclear protein STH1/NPS1, : 1 : 9 entities Detail

Interaction partners 2. Nuclear protein STH1/NPS1, : 92 interactors Detail

More details for 92 interactors identified by 15 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Agilent DD2 - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM [U-100% 13C; U-100% 15N] Taf14ET, 1.5 mM [U-100% 13C; U-100% 15N] Sth1EBMC, 20 mM Phosphate buffer, 100 mM sodium chloride, 1 % v/v inhabitor cocktail, 0.04 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

3 2D 1H-13C HSQC aromatic

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

4 3D HNCO

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

5 3D HNCACO

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

6 3D HN(CO)CA

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

7 3D HNHA

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

8 3D HBHA(CO)NH

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

9 3D CBCA(CO)NH

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

10 3D HNCACB

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

11 3D CBCA(CO)NH

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

12 3D HNCA

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

13 3D 1H-13C NOESY aliphatic

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

14 3D 1H-15N NOESY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

15 3D CCH-TOCSY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

16 3D HCCH-TOCSY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

17 3D 1H-13C NOESY aromatic

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Phosphate buffer	natural abundance	buffer	20 mM
2	Sth1EBMC	[U-100% 13C; U-100% 15N]	protein	1.5 mM
3	Taf14ET	[U-100% 13C; U-100% 15N]	protein	1 mM
4	inhabitor cocktail	natural abundance		1 % v/v
5	sodium azide	natural abundance		0.04 %
6	sodium chloride	natural abundance	salt	100 mM
7	H2O	natural abundance	solvent	90 %
8	D2O	[U-2H]	solvent	10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36309_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36309_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1530		98.6 (chain: 1, length: 72), 85.0 (chain: 2, length: 60)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 72, Sequence coverage: 98.6%
  - Entity_assembly_ID: 2, Chain length: 60, Sequence coverage: 85.0%
- Total number of assignments
  - ¹H chemical shifts: 879
  - ¹³C chemical shifts: 530
  - ¹⁵N chemical shifts: 121
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
  - Chain_ID: 2

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
15	THR	HG1	5.916

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	436	426	97.7
¹³C chemical shifts	329	314	95.4
¹⁵N chemical shifts	78	76	97.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	147	143	97.3
¹³C chemical shifts	144	141	97.9
¹⁵N chemical shifts	70	68	97.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	289	283	97.9
¹³C chemical shifts	185	173	93.5
¹⁵N chemical shifts	8	8	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	48	100.0
¹³C chemical shifts	48	48	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	20	83.3
¹³C chemical shifts	23	12	52.2
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	386	304	78.8
¹³C chemical shifts	272	216	79.4
¹⁵N chemical shifts	62	45	72.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	119	93	78.2
¹³C chemical shifts	120	97	80.8
¹⁵N chemical shifts	57	41	71.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	267	211	79.0
¹³C chemical shifts	152	119	78.3
¹⁵N chemical shifts	5	4	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	28	26	92.9
¹³C chemical shifts	28	23	82.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	0	0.0
¹³C chemical shifts	4	0	0.0

Keywords TRANSCRIPTION, phase separation, protein interaction module

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Found 1 Document (3.01 seconds)

BMRB: 36309

Sample

Related entities 1. Transcription initiation factor TFIID subunit 14, : 1 : 3 entities Detail

Related entities 2. Nuclear protein STH1/NPS1, : 1 : 9 entities Detail

Interaction partners 2. Nuclear protein STH1/NPS1, : 92 interactors Detail

Experiments performed 17 experiments Detail

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Chemical shift validation 4 contents Detail