BMRBj - BMREntryMaster

Found 1 Document (0.573 seconds)

BMRB: 36368

7CQ1

Solution structure of the C-terminal domain of Mycobacterium Tuberculosis ribosome maturation factor protein RimM

Authors

Lin, D., Zhang, H.

Assembly

Ribosome maturation factor RimM

Entity

1. Ribosome maturation factor RimM (polymer, Thiol state: not present), 85 monomers, 9281.321 Da Detail

MDTYYDHQLV GLMVQTATGE GVGVVTEVVH TAAGELLAVK RDSDEVLVPF VRAIVTSVSL DDGIVEIDPP HGLLNLELEH HHHHH

Formula weight

9281.321 Da

Source organism

Mycobacterium tuberculosis H37Rv

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 82.5 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.5 % (769 of 932)	80.1 % (374 of 467)	84.9 % (321 of 378)	85.1 % (74 of 87)
Backbone	90.9 % (458 of 504)	90.8 % (158 of 174)	91.1 % (226 of 248)	90.2 % (74 of 82)
Sidechain	75.5 % (382 of 506)	73.7 % (216 of 293)	79.8 % (166 of 208)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)
Methyl	96.2 % (125 of 130)	92.3 % (60 of 65)	100.0 % (65 of 65)

1. entity 1

MDTYYDHQLV GLMVQTATGE GVGVVTEVVH TAAGELLAVK RDSDEVLVPF VRAIVTSVSL DDGIVEIDPP HGLLNLELEH HHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.6 mM C-terminal domain of ribosome maturation factor RimM, 20 mM potassium phosphate, 100 mM potassium chloride, 0.02 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	C-terminal domain of ribosome maturation factor RimM	natural abundance	protein	0.6 mM
2	potassium chloride	natural abundance	salt	100 mM
3	potassium phosphate	natural abundance	buffer	20 mM
4	sodium azide	natural abundance		0.02 % w/v
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.6 mM [U-99% 15N] C-terminal domain of ribosome maturation factor RimM, 20 mM potassium phosphate, 100 mM potassium chloride, 0.02 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	C-terminal domain of ribosome maturation factor RimM	[U-99% 15N]	protein	0.6 mM
8	potassium chloride	natural abundance	salt	100 mM
9	potassium phosphate	natural abundance	buffer	20 mM
10	sodium azide	natural abundance		0.02 % w/v
11	H2O	natural abundance	solvent	90 %
12	D2O	[U-2H]	solvent	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.6 mM [U-99% 13C; U-99% 15N] C-terminal domain of ribosome maturation factor RimM, 20 mM potassium phosphate, 100 mM potassium chloride, 0.02 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

Release date

2021-02-02

Citation

Structural Basis for the C-Terminal Domain of Mycobacterium tuberculosis Ribosome Maturation Factor RimM to Bind Ribosomal Protein S19
Zhang, H., Lin, D.
Biomolecules (2021), 11, PubMed 33919647 , DOI 10.3390/biom11040597 , Abstract

Related entities 1. Ribosome maturation factor RimM, : 1 : 2 : 103 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.6 mM [U-99% 15N] C-terminal domain of ribosome maturation factor RimM, 20 mM potassium phosphate, 100 mM potassium chloride, 0.02 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	C-terminal domain of ribosome maturation factor RimM	[U-99% 15N]	protein	0.6 mM
8	potassium chloride	natural abundance	salt	100 mM
9	potassium phosphate	natural abundance	buffer	20 mM
10	sodium azide	natural abundance		0.02 % w/v
11	H2O	natural abundance	solvent	90 %
12	D2O	[U-2H]	solvent	10 %

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	C-terminal domain of ribosome maturation factor RimM	[U-99% 15N]	protein	0.6 mM
8	potassium chloride	natural abundance	salt	100 mM
9	potassium phosphate	natural abundance	buffer	20 mM
10	sodium azide	natural abundance		0.02 % w/v
11	H2O	natural abundance	solvent	90 %
12	D2O	[U-2H]	solvent	10 %

3 3D HNCACB

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2, Details 0.6 mM [U-99% 13C; U-99% 15N] C-terminal domain of ribosome maturation factor RimM, 20 mM potassium phosphate, 100 mM potassium chloride, 0.02 % w/v sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

5 3D HNCA

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

6 3D HN(CO)CA

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

7 3D HNCO

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

8 3D HN(CA)CO

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

9 3D HCCH-COSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

10 3D H(CCO)NH

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

11 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

12 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

13 3D C(CO)NH

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

14 3D 15N-edited NOESY-HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	C-terminal domain of ribosome maturation factor RimM	[U-99% 15N]	protein	0.6 mM
8	potassium chloride	natural abundance	salt	100 mM
9	potassium phosphate	natural abundance	buffer	20 mM
10	sodium azide	natural abundance		0.02 % w/v
11	H2O	natural abundance	solvent	90 %
12	D2O	[U-2H]	solvent	10 %

15 3D 13C-edited NOESY-HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
13	C-terminal domain of ribosome maturation factor RimM	[U-99% 13C; U-99% 15N]	protein	0.6 mM
14	potassium chloride	natural abundance	salt	100 mM
15	potassium phosphate	natural abundance	buffer	20 mM
16	sodium azide	natural abundance		0.02 % w/v
17	H2O	natural abundance	solvent	90 %
18	D2O	[U-2H]	solvent	10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36368_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36368_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	889		91.8 (chain: 1, length: 85)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 85, Sequence coverage: 91.8%
- Total number of assignments
  - ¹H chemical shifts: 494
  - ¹³C chemical shifts: 321
  - ¹⁵N chemical shifts: 74
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	467	374	80.1
¹³C chemical shifts	378	321	84.9
¹⁵N chemical shifts	87	74	85.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	174	158	90.8
¹³C chemical shifts	170	155	91.2
¹⁵N chemical shifts	82	74	90.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	293	216	73.7
¹³C chemical shifts	208	166	79.8
¹⁵N chemical shifts	5	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	60	89.6
¹³C chemical shifts	67	65	97.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	0	0.0
¹³C chemical shifts	31	0	0.0

Keywords 30S subunit, PROTEIN BINDING, Ribosome maturation

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Found 1 Document (0.573 seconds)

BMRB: 36368

Sample #1

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Related entities 1. Ribosome maturation factor RimM, : 1 : 2 : 103 entities Detail

Experiments performed 15 experiments Detail

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Chemical shift validation 3 contents Detail