BMRB: 36417

Prion Derived Tetrapeptide Stabilizes Thermolabile Insulin via Conformational Trapping

Authors

Banerjee, N.

Assembly

Insulin Chain A, Insulin B chain, IS1

Entity

1. Insulin Chain A, Insulin B chain, IS1, entity 1 (polymer, Thiol state: all free), 21 monomers, 2339.643 Da Detail

GIVEQCCASV CSLYQLENYC N

2. Insulin Chain A, Insulin B chain, IS1, entity 2 (polymer, Thiol state: all free), 30 monomers, 3399.893 Da Detail

FVNQHLCGSH LVEALYLVCG ERGFFYTPKA

3. Insulin Chain A, Insulin B chain, IS1, entity 3 (polymer, Thiol state: not present), 4 monomers, 599.6792 Da Detail

VYYR

Total weight

6339.2153 Da

Max. entity weight

3399.893 Da

Source organism

Bos taurus , Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.1 %, Completeness: 80.2 %, Completeness (bb): 84.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.2 % (263 of 328)	80.2 % (263 of 328)
Backbone	84.1 % (95 of 113)	84.1 % (95 of 113)
Sidechain	78.1 % (168 of 215)	78.1 % (168 of 215)
Aromatic	44.2 % (19 of 43)	44.2 % (19 of 43)
Methyl	80.0 % (24 of 30)	80.0 % (24 of 30)

1. entity 1

GIVEQCCASV CSLYQLENYC N

2. entity 2

FVNQHLCGSH LVEALYLVCG ERGFFYTPKA

3. entity 3

VYYR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.6, Details 1 mM Proton Bovine Insulin, 1 mM Proton-15N, 13C Peptide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Bovine Insulin	Proton	protein	1 mM
2	Peptide	Proton-15N, 13C	protein	1 mM
3	Citrate-Phoshate	natural abundance	buffer	1 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

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Release date

2021-04-29

Citation

Prion-derived tetrapeptide stabilizes thermolabile insulin via conformational trapping
Mukherjee, M., Das, D., Sarkar, J., Banerjee, N., Jana, J., Bhat, J., Reddy G, J., Bharatam, J., Chattopadhyay, S., Chatterjee, S., Chakrabarti, P.
iScience (2021), 24, 102573-102573, PubMed 34142060 , DOI 10.1016/j.isci.2021.102573 , Abstract

Related entities 1. Insulin Chain A, Insulin B chain, IS1, entity 1, : 2 : 1 : 8 : 111 entities Detail

Related entities 2. Insulin Chain A, Insulin B chain, IS1, entity 2, : 9 : 4 : 19 : 77 entities Detail

Experiments performed 3 experiments Detail