BMRB: 36422

T-hairpin structure found in the RNA element involved in the piRNA biogenesis

Authors

Takase, N., Kawai, G.

Assembly

T-hairpin RNA

Entity

1. T-hairpin RNA (polymer, Thiol state: not present), 16 monomers, 5139.979 Da Detail

UUGGUUUGAU UCCAAG

Formula weight

5139.979 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 53.2 %, Completeness (bb): 49.0 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	53.2 % (74 of 139)	53.2 % (74 of 139)
Suger, PO4	49.0 % (47 of 96)	49.0 % (47 of 96)
Nucleobase	62.8 % (27 of 43)	62.8 % (27 of 43)
Aromatic	59.0 % (23 of 39)	59.0 % (23 of 39)

1. RNA (5'-R(*UP*UP*GP*GP*UP*UP*UP*GP*AP*UP*UP*CP*CP*AP*AP*G)-3')

UUGGUUUGAU UCCAAG

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.3 mM None RNA (5'-R(*UP*UP*GP*GP*UP*UP*UP*GP*AP*UP*UP*CP*CP*AP*AP*G)-3'), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA (5'-R(*UP*UP*GP*GP*UP*UP*UP*GP*AP*UP*UP*CP*CP*AP*AP*G)-3')	natural abundance	RNA	0.3 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	50 mM
4	H2O	natural abundance	solvent	95 %
5	D2O	[U-2H]	solvent	5 %

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Release date

2022-03-01

Citation

T-hairpin structure found in the RNA element involved in piRNA biogenesis
Takase, N., Otsu, M., Hirakata, S., Ishizu, H., Siomi, M.C., Kawai, G.
RNA (2022), 28, 541-550, PubMed 34987083 , DOI 10.1261/rna.078967.121 , Abstract

Experiments performed 2 experiments Detail