Solution structure of apo-WhiB4 from Mycobacterium tuberculosis
MSGTRPAARR TNLTAAQNVV RSVDAEERIA WVSKALCRTT DPDELFVRGA AQRKAAVICR HCPVMQECAA DALDNKVEFG VWGGMTERQR RALLKQHPEV VSWSDYLEKR KRRTGTAGLE HHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.8 % (1241 of 1446) | 85.3 % (636 of 746) | 87.5 % (483 of 552) | 82.4 % (122 of 148) |
Backbone | 90.5 % (677 of 748) | 90.2 % (230 of 255) | 90.6 % (336 of 371) | 91.0 % (111 of 122) |
Sidechain | 82.3 % (672 of 817) | 82.7 % (406 of 491) | 85.0 % (255 of 300) | 42.3 % (11 of 26) |
Aromatic | 43.8 % (42 of 96) | 43.8 % (21 of 48) | 40.0 % (18 of 45) | 100.0 % (3 of 3) |
Methyl | 97.0 % (128 of 132) | 97.0 % (64 of 66) | 97.0 % (64 of 66) |
1. Transcriptional regulator WhiB4
MSGTRPAARR TNLTAAQNVV RSVDAEERIA WVSKALCRTT DPDELFVRGA AQRKAAVICR HCPVMQECAA DALDNKVEFG VWGGMTERQR RALLKQHPEV VSWSDYLEKR KRRTGTAGLE HHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM [U-100% 13C; U-100% 15N] WhiB4, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WhiB4 | [U-100% 13C; U-100% 15N] | protein | 0.4 mM |
2 | NaCl | natural abundance | salt | 50 mM |
3 | H2O | natural abundance | solvent | 95 % |
4 | D2O | [U-2H] | solvent | 5 % |