BMRBj - BMREntryMaster

Found 1 Document (2.259 seconds)

BMRB: 36427

7F7X

Protein complex between phosphorylated ubiquitin and Ubqln2 UBA

Authors

Qin, L.Y., Dong, X., Tang, C.

Assembly

Polyubiquitin-B, Ubiquilin-2

Entity

1. Polyubiquitin-B, Ubiquilin-2, entity 1 (polymer, Thiol state: not present), 76 monomers, 8644.710 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKEXTLHLV LRLRGG

2. Polyubiquitin-B, Ubiquilin-2, entity 2 (polymer, Thiol state: not present), 45 monomers, 4868.442 Da Detail

GPEVRFQQQL EQLNAMGFLN REANLQALIA TGGDINAAIE RLLGS

Total weight

13513.152 Da

Max. entity weight

8644.71 Da

Entity Connection

covalent 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:GLU64:C	1:SEP65:N
2	covalent	sing	1:SEP65:C	1:THR66:N

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 449, ¹³C: 295, ¹⁵N: 69 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Ambig. code
1	1	1	MET	HA	H	4.154	1
2	1	1	MET	HB2	H	2.023	1
3	1	1	MET	HB3	H	2.053	1
4	1	1	MET	HG2	H	2.496	1
5	1	1	MET	HG3	H	2.519	1
6	1	1	MET	HE1	H	1.625	1
7	1	1	MET	HE2	H	1.625	1
8	1	1	MET	HE3	H	1.625	1
9	1	1	MET	C	C	170.509	1
10	1	1	MET	CA	C	54.488	1
11	1	1	MET	CB	C	33.221	1
12	1	1	MET	CG	C	30.997	1
13	1	1	MET	CE	C	17.646	1
14	1	2	GLN	H	H	8.871	1
15	1	2	GLN	HA	H	5.327	1
16	1	2	GLN	HB2	H	1.631	1
17	1	2	GLN	HB3	H	1.852	1
18	1	2	GLN	HG2	H	2.225	1
19	1	2	GLN	HG3	H	2.225	1
20	1	2	GLN	C	C	175.911	1
21	1	2	GLN	CA	C	54.905	1
22	1	2	GLN	CB	C	30.608	1
23	1	2	GLN	N	N	122.945	1
24	1	3	ILE	H	H	8.289	1
25	1	3	ILE	HA	H	4.156	1
26	1	3	ILE	HB	H	1.729	1
27	1	3	ILE	HG12	H	1.007	1
28	1	3	ILE	HG13	H	1.007	1
29	1	3	ILE	HG21	H	0.678	1
30	1	3	ILE	HG22	H	0.678	1
31	1	3	ILE	HG23	H	0.678	1
32	1	3	ILE	HD11	H	0.589	1
33	1	3	ILE	HD12	H	0.589	1
34	1	3	ILE	HD13	H	0.589	1
35	1	3	ILE	C	C	172.520	1
36	1	3	ILE	CA	C	59.619	1
37	1	3	ILE	CB	C	42.305	1
38	1	3	ILE	CG1	C	25.110	1
39	1	3	ILE	CG2	C	17.935	1
40	1	3	ILE	CD1	C	14.239	1
41	1	3	ILE	N	N	115.045	1
42	1	4	PHE	H	H	8.367	1
43	1	4	PHE	HA	H	5.587	1
44	1	4	PHE	HB2	H	3.019	1
45	1	4	PHE	HB3	H	2.814	1
46	1	4	PHE	HD1	H	7.049	1
47	1	4	PHE	HD2	H	7.049	1
48	1	4	PHE	C	C	175.046	1
49	1	4	PHE	CA	C	55.528	1
50	1	4	PHE	CB	C	41.133	1
51	1	4	PHE	CD2	C	131.065	1
52	1	4	PHE	N	N	118.658	1
53	1	5	VAL	H	H	9.277	1
54	1	5	VAL	HA	H	4.871	1
55	1	5	VAL	HB	H	1.882	1
56	1	5	VAL	HG11	H	0.680	1
57	1	5	VAL	HG12	H	0.680	1
58	1	5	VAL	HG13	H	0.680	1
59	1	5	VAL	HG21	H	0.717	1
60	1	5	VAL	HG22	H	0.717	1
61	1	5	VAL	HG23	H	0.717	1
62	1	5	VAL	C	C	174.796	1
63	1	5	VAL	CA	C	60.356	1
64	1	5	VAL	CB	C	34.175	1
65	1	5	VAL	CG1	C	21.066	1
66	1	5	VAL	CG2	C	22.436	1
67	1	5	VAL	N	N	121.187	1
68	1	6	LYS	H	H	8.957	1
69	1	6	LYS	HA	H	5.263	1
70	1	6	LYS	HB2	H	1.630	1
71	1	6	LYS	HB3	H	1.630	1
72	1	6	LYS	HG2	H	1.399	1
73	1	6	LYS	HG3	H	1.360	1
74	1	6	LYS	HD2	H	1.557	1
75	1	6	LYS	HD3	H	1.557	1
76	1	6	LYS	HE2	H	2.892	1
77	1	6	LYS	HE3	H	2.892	1
78	1	6	LYS	C	C	177.023	1
79	1	6	LYS	CA	C	54.650	1
80	1	6	LYS	CB	C	34.449	1
81	1	6	LYS	CG	C	25.100	1
82	1	6	LYS	CD	C	29.373	1
83	1	6	LYS	CE	C	42.172	1
84	1	6	LYS	N	N	128.110	1
85	1	7	THR	H	H	8.723	1
86	1	7	THR	HA	H	4.992	1
87	1	7	THR	HB	H	4.859	1
88	1	7	THR	HG21	H	1.161	1
89	1	7	THR	HG22	H	1.161	1
90	1	7	THR	HG23	H	1.161	1
91	1	7	THR	C	C	176.881	1
92	1	7	THR	CA	C	60.489	1
93	1	7	THR	CB	C	70.676	1
94	1	7	THR	CG2	C	21.693	1
95	1	7	THR	N	N	115.439	1
96	1	8	LEU	H	H	9.145	1
97	1	8	LEU	CA	C	57.478	1
98	1	8	LEU	CB	C	41.839	1
99	1	8	LEU	N	N	121.561	1
100	1	9	THR	HA	H	4.439	1
101	1	9	THR	HG21	H	1.233	1
102	1	9	THR	HG22	H	1.233	1
103	1	9	THR	HG23	H	1.233	1
104	1	9	THR	C	C	175.448	1
105	1	9	THR	CG2	C	22.012	1
106	1	10	GLY	H	H	7.853	1
107	1	10	GLY	HA2	H	3.580	1
108	1	10	GLY	HA3	H	4.312	1
109	1	10	GLY	C	C	173.904	1
110	1	10	GLY	CA	C	45.365	1
111	1	10	GLY	N	N	109.441	1
112	1	11	LYS	H	H	7.303	1
113	1	11	LYS	HA	H	4.339	1
114	1	11	LYS	HB2	H	1.717	1
115	1	11	LYS	HB3	H	1.717	1
116	1	11	LYS	HG2	H	1.310	1
117	1	11	LYS	HG3	H	1.310	1
118	1	11	LYS	HD2	H	1.652	1
119	1	11	LYS	C	C	175.678	1
120	1	11	LYS	CA	C	56.355	1
121	1	11	LYS	CB	C	33.362	1
122	1	11	LYS	CG	C	25.146	1
123	1	11	LYS	CD	C	29.642	1
124	1	11	LYS	N	N	122.186	1
125	1	12	THR	H	H	8.653	1
126	1	12	THR	HA	H	5.037	1
127	1	12	THR	HB	H	3.919	1
128	1	12	THR	HG21	H	1.054	1
129	1	12	THR	HG22	H	1.054	1
130	1	12	THR	HG23	H	1.054	1
131	1	12	THR	C	C	174.227	1
132	1	12	THR	CA	C	62.304	1
133	1	12	THR	CB	C	69.933	1
134	1	12	THR	CG2	C	22.169	1
135	1	12	THR	N	N	120.842	1
136	1	13	ILE	H	H	9.608	1
137	1	13	ILE	HA	H	4.489	1
138	1	13	ILE	HB	H	1.842	1
139	1	13	ILE	HG12	H	1.450	1
140	1	13	ILE	HG13	H	1.450	1
141	1	13	ILE	HG21	H	0.860	1
142	1	13	ILE	HG22	H	0.860	1
143	1	13	ILE	HG23	H	0.860	1
144	1	13	ILE	HD11	H	0.847	1
145	1	13	ILE	HD12	H	0.847	1
146	1	13	ILE	HD13	H	0.847	1
147	1	13	ILE	C	C	175.097	1
148	1	13	ILE	CA	C	60.054	1
149	1	13	ILE	CB	C	40.893	1
150	1	13	ILE	CG1	C	27.120	1
151	1	13	ILE	CG2	C	17.696	1
152	1	13	ILE	CD1	C	14.565	1
153	1	13	ILE	N	N	128.084	1
154	1	14	THR	H	H	8.744	1
155	1	14	THR	HA	H	4.934	1
156	1	14	THR	HB	H	3.934	1
157	1	14	THR	HG21	H	1.063	1
158	1	14	THR	HG22	H	1.063	1
159	1	14	THR	HG23	H	1.063	1
160	1	14	THR	C	C	173.691	1
161	1	14	THR	CA	C	61.989	1
162	1	14	THR	CB	C	69.825	1
163	1	14	THR	CG2	C	21.928	1
164	1	14	THR	N	N	121.598	1
165	1	15	LEU	H	H	8.704	1
166	1	15	LEU	HA	H	4.714	1
167	1	15	LEU	HB2	H	1.377	1
168	1	15	LEU	HB3	H	1.243	1
169	1	15	LEU	HD11	H	0.697	1
170	1	15	LEU	HD12	H	0.697	1
171	1	15	LEU	HD13	H	0.697	1
172	1	15	LEU	HD21	H	1.378	1
173	1	15	LEU	HD22	H	1.378	1
174	1	15	LEU	HD23	H	1.378	1
175	1	15	LEU	C	C	174.504	1
176	1	15	LEU	CA	C	52.746	1
177	1	15	LEU	CB	C	45.971	1
178	1	15	LEU	CD1	C	26.993	1
179	1	15	LEU	CD2	C	24.260	1
180	1	15	LEU	N	N	124.921	1
181	1	16	GLU	H	H	8.128	1
182	1	16	GLU	HA	H	4.855	1
183	1	16	GLU	HB2	H	1.898	1
184	1	16	GLU	HB3	H	1.898	1
185	1	16	GLU	HG2	H	2.130	1
186	1	16	GLU	HG3	H	2.130	1
187	1	16	GLU	C	C	175.707	1
188	1	16	GLU	CA	C	55.032	1
189	1	16	GLU	CB	C	29.756	1
190	1	16	GLU	CG	C	35.710	1
191	1	16	GLU	N	N	122.703	1
192	1	17	VAL	H	H	8.963	1
193	1	17	VAL	HA	H	4.666	1
194	1	17	VAL	HB	H	2.291	1
195	1	17	VAL	HG11	H	0.409	1
196	1	17	VAL	HG12	H	0.409	1
197	1	17	VAL	HG13	H	0.409	1
198	1	17	VAL	HG21	H	0.702	1
199	1	17	VAL	HG22	H	0.702	1
200	1	17	VAL	HG23	H	0.702	1
201	1	17	VAL	C	C	174.002	1
202	1	17	VAL	CA	C	58.549	1
203	1	17	VAL	CB	C	36.384	1
204	1	17	VAL	CG1	C	19.389	1
205	1	17	VAL	CG2	C	22.261	1
206	1	17	VAL	N	N	117.755	1
207	1	18	GLU	H	H	8.612	1
208	1	18	GLU	HA	H	5.014	1
209	1	18	GLU	HB2	H	2.127	1
210	1	18	GLU	HB3	H	1.616	1
211	1	18	GLU	CA	C	52.787	1
212	1	18	GLU	CB	C	30.856	1
213	1	18	GLU	N	N	119.278	1
214	1	19	PRO	HA	H	4.113	1
215	1	19	PRO	HB2	H	2.400	1
216	1	19	PRO	HB3	H	2.400	1
217	1	19	PRO	HG2	H	1.998	1
218	1	19	PRO	HG3	H	1.998	1
219	1	19	PRO	HD2	H	3.755	1
220	1	19	PRO	HD3	H	3.755	1
221	1	19	PRO	C	C	175.172	1
222	1	19	PRO	CB	C	31.811	1
223	1	19	PRO	CG	C	28.141	1
224	1	19	PRO	CD	C	50.511	1
225	1	20	SER	H	H	7.041	1
226	1	20	SER	HA	H	4.132	1
227	1	20	SER	HB2	H	3.748	1
228	1	20	SER	HB3	H	3.748	1
229	1	20	SER	C	C	174.557	1
230	1	20	SER	CA	C	57.415	1
231	1	20	SER	CB	C	63.545	1
232	1	20	SER	N	N	103.568	1
233	1	21	ASP	H	H	8.107	1
234	1	21	ASP	HA	H	4.657	1
235	1	21	ASP	HB2	H	2.899	1
236	1	21	ASP	HB3	H	2.479	1
237	1	21	ASP	C	C	176.258	1
238	1	21	ASP	CA	C	55.927	1
239	1	21	ASP	CB	C	40.893	1
240	1	21	ASP	N	N	124.236	1
241	1	22	THR	H	H	7.891	1
242	1	22	THR	HA	H	4.902	1
243	1	22	THR	HB	H	4.808	1
244	1	22	THR	HG21	H	1.242	1
245	1	22	THR	HG22	H	1.242	1
246	1	22	THR	HG23	H	1.242	1
247	1	22	THR	C	C	176.630	1
248	1	22	THR	CA	C	59.684	1
249	1	22	THR	CB	C	71.221	1
250	1	22	THR	CG2	C	22.284	1
251	1	22	THR	N	N	109.270	1
252	1	23	ILE	H	H	8.590	1
253	1	23	ILE	HA	H	3.602	1
254	1	23	ILE	HB	H	2.329	1
255	1	23	ILE	HG12	H	1.280	1
256	1	23	ILE	HG13	H	1.808	1
257	1	23	ILE	HD11	H	0.735	1
258	1	23	ILE	HD12	H	0.735	1
259	1	23	ILE	HD13	H	0.735	1
260	1	23	ILE	CA	C	62.427	1
261	1	23	ILE	CB	C	34.407	1
262	1	23	ILE	CG1	C	28.276	1
263	1	23	ILE	CG2	C	18.589	1
264	1	23	ILE	CD1	C	9.548	1
265	1	23	ILE	N	N	121.689	1
266	1	24	GLU	H	H	10.107	1
267	1	24	GLU	HA	H	3.854	1
268	1	24	GLU	HB2	H	1.989	1
269	1	24	GLU	HB3	H	1.989	1
270	1	24	GLU	HG2	H	2.338	1
271	1	24	GLU	HG3	H	2.338	1
272	1	24	GLU	C	C	178.910	1
273	1	24	GLU	CA	C	60.863	1
274	1	24	GLU	CB	C	28.708	1
275	1	24	GLU	CG	C	36.419	1
276	1	24	GLU	N	N	121.323	1
277	1	25	ASN	H	H	7.955	1
278	1	25	ASN	HA	H	4.528	1
279	1	25	ASN	HB2	H	2.815	1
280	1	25	ASN	HB3	H	3.198	1
281	1	25	ASN	C	C	178.239	1
282	1	25	ASN	CA	C	55.981	1
283	1	25	ASN	CB	C	38.376	1
284	1	25	ASN	N	N	121.455	1
285	1	26	VAL	H	H	8.189	1
286	1	26	VAL	HA	H	3.361	1
287	1	26	VAL	HB	H	2.316	1
288	1	26	VAL	HG11	H	0.932	1
289	1	26	VAL	HG12	H	0.932	1
290	1	26	VAL	HG13	H	0.932	1
291	1	26	VAL	HG21	H	0.662	1
292	1	26	VAL	HG22	H	0.662	1
293	1	26	VAL	HG23	H	0.662	1
294	1	26	VAL	C	C	177.838	1
295	1	26	VAL	CA	C	67.735	1
296	1	26	VAL	CB	C	30.845	1
297	1	26	VAL	CG1	C	21.712	1
298	1	26	VAL	CG2	C	23.630	1
299	1	26	VAL	N	N	122.574	1
300	1	27	LYS	H	H	8.565	1
301	1	27	LYS	HA	H	4.578	1
302	1	27	LYS	HB2	H	1.983	1
303	1	27	LYS	HB3	H	1.989	1
304	1	27	LYS	HG2	H	1.433	1
305	1	27	LYS	HG3	H	1.433	1
306	1	27	LYS	HE2	H	2.626	1
307	1	27	LYS	C	C	180.477	1
308	1	27	LYS	CA	C	59.217	1
309	1	27	LYS	CB	C	33.681	1
310	1	27	LYS	CG	C	26.214	1
311	1	27	LYS	CD	C	30.590	1
312	1	27	LYS	CE	C	42.426	1
313	1	27	LYS	N	N	118.939	1
314	1	28	ALA	H	H	7.962	1
315	1	28	ALA	HA	H	4.130	1
316	1	28	ALA	HB1	H	1.603	1
317	1	28	ALA	HB2	H	1.603	1
318	1	28	ALA	HB3	H	1.603	1
319	1	28	ALA	C	C	180.172	1
320	1	28	ALA	CA	C	55.395	1
321	1	28	ALA	CB	C	17.656	1
322	1	28	ALA	N	N	123.621	1
323	1	29	LYS	H	H	7.927	1
324	1	29	LYS	HA	H	4.172	1
325	1	29	LYS	HB2	H	2.090	1
326	1	29	LYS	HG2	H	1.589	1
327	1	29	LYS	HD2	H	1.875	1
328	1	29	LYS	C	C	180.296	1
329	1	29	LYS	CA	C	59.835	1
330	1	29	LYS	CB	C	33.292	1
331	1	29	LYS	CG	C	26.656	1
332	1	29	LYS	CD	C	30.379	1
333	1	29	LYS	CE	C	42.432	1
334	1	29	LYS	N	N	120.586	1
335	1	30	ILE	H	H	8.332	1
336	1	30	ILE	HA	H	3.475	1
337	1	30	ILE	HB	H	2.311	1
338	1	30	ILE	HG21	H	0.705	1
339	1	30	ILE	HG22	H	0.705	1
340	1	30	ILE	HG23	H	0.705	1
341	1	30	ILE	HD11	H	0.649	1
342	1	30	ILE	HD12	H	0.649	1
343	1	30	ILE	HD13	H	0.649	1
344	1	30	ILE	C	C	178.119	1
345	1	30	ILE	CA	C	66.229	1
346	1	30	ILE	CB	C	36.758	1
347	1	30	ILE	CG2	C	17.136	1
348	1	30	ILE	CD1	C	15.217	1
349	1	30	ILE	N	N	121.759	1
350	1	31	GLN	H	H	8.552	1
351	1	31	GLN	HA	H	3.792	1
352	1	31	GLN	HB2	H	1.912	1
353	1	31	GLN	HB3	H	2.465	1
354	1	31	GLN	HG2	H	2.283	1
355	1	31	GLN	HG3	H	2.283	1
356	1	31	GLN	C	C	178.859	1
357	1	31	GLN	CA	C	60.088	1
358	1	31	GLN	CB	C	27.687	1
359	1	31	GLN	CG	C	33.915	1
360	1	31	GLN	N	N	123.703	1
361	1	32	ASP	H	H	8.070	1
362	1	32	ASP	HA	H	4.314	1
363	1	32	ASP	HB2	H	2.768	1
364	1	32	ASP	HB3	H	2.768	1
365	1	32	ASP	C	C	177.253	1
366	1	32	ASP	CA	C	57.487	1
367	1	32	ASP	CB	C	41.061	1
368	1	32	ASP	N	N	120.006	1
369	1	33	LYS	H	H	7.458	1
370	1	33	LYS	HA	H	4.287	1
371	1	33	LYS	HB2	H	1.922	1
372	1	33	LYS	HG2	H	1.611	1
373	1	33	LYS	C	C	177.745	1
374	1	33	LYS	CA	C	58.307	1
375	1	33	LYS	CB	C	33.985	1
376	1	33	LYS	CG	C	25.302	1
377	1	33	LYS	CD	C	29.012	1
378	1	33	LYS	CE	C	42.372	1
379	1	33	LYS	N	N	115.782	1
380	1	34	GLU	H	H	8.734	1
381	1	34	GLU	HA	H	4.546	1
382	1	34	GLU	HB2	H	1.660	1
383	1	34	GLU	HB3	H	1.660	1
384	1	34	GLU	HG2	H	2.121	1
385	1	34	GLU	HG3	H	2.121	1
386	1	34	GLU	C	C	177.915	1
387	1	34	GLU	CA	C	55.294	1
388	1	34	GLU	CB	C	33.267	1
389	1	34	GLU	CG	C	36.522	1
390	1	34	GLU	N	N	114.419	1
391	1	35	GLY	H	H	8.522	1
392	1	35	GLY	HA2	H	3.900	1
393	1	35	GLY	HA3	H	4.086	1
394	1	35	GLY	C	C	173.875	1
395	1	35	GLY	CA	C	46.038	1
396	1	35	GLY	N	N	109.037	1
397	1	36	ILE	H	H	6.160	1
398	1	36	ILE	HA	H	4.406	1
399	1	36	ILE	HB	H	1.388	1
400	1	36	ILE	HG12	H	1.064	1
401	1	36	ILE	HG21	H	0.897	1
402	1	36	ILE	HG22	H	0.897	1
403	1	36	ILE	HG23	H	0.897	1
404	1	36	ILE	HD11	H	0.764	1
405	1	36	ILE	HD12	H	0.764	1
406	1	36	ILE	HD13	H	0.764	1
407	1	36	ILE	CA	C	57.799	1
408	1	36	ILE	CB	C	40.508	1
409	1	36	ILE	CG2	C	17.623	1
410	1	36	ILE	N	N	120.523	1
411	1	38	PRO	HA	H	4.077	1
412	1	38	PRO	HB2	H	2.150	1
413	1	38	PRO	HB3	H	2.150	1
414	1	38	PRO	HG2	H	1.595	1
415	1	38	PRO	HD2	H	3.683	1
416	1	38	PRO	HD3	H	3.683	1
417	1	38	PRO	C	C	178.204	1
418	1	38	PRO	CA	C	66.264	1
419	1	38	PRO	CB	C	32.927	1
420	1	38	PRO	CG	C	27.798	1
421	1	38	PRO	CD	C	51.144	1
422	1	39	ASP	H	H	8.577	1
423	1	39	ASP	HA	H	4.389	1
424	1	39	ASP	HB2	H	2.694	1
425	1	39	ASP	HB3	H	2.694	1
426	1	39	ASP	C	C	177.014	1
427	1	39	ASP	CA	C	55.780	1
428	1	39	ASP	CB	C	39.796	1
429	1	39	ASP	N	N	113.756	1
430	1	40	GLN	H	H	7.837	1
431	1	40	GLN	HA	H	4.395	1
432	1	40	GLN	HB2	H	1.787	1
433	1	40	GLN	HB3	H	1.798	1
434	1	40	GLN	HG2	H	2.400	1
435	1	40	GLN	HG3	H	2.400	1
436	1	40	GLN	C	C	175.292	1
437	1	40	GLN	CA	C	55.603	1
438	1	40	GLN	CB	C	30.037	1
439	1	40	GLN	CG	C	34.398	1
440	1	40	GLN	N	N	116.978	1
441	1	41	GLN	H	H	7.498	1
442	1	41	GLN	HA	H	4.182	1
443	1	41	GLN	HB2	H	1.901	1
444	1	41	GLN	HB3	H	1.901	1
445	1	41	GLN	HG2	H	2.502	1
446	1	41	GLN	HG3	H	2.502	1
447	1	41	GLN	C	C	176.017	1
448	1	41	GLN	CA	C	56.725	1
449	1	41	GLN	CB	C	31.402	1
450	1	41	GLN	CG	C	33.588	1
451	1	41	GLN	N	N	118.197	1
452	1	42	ARG	H	H	8.501	1
453	1	42	ARG	HA	H	4.449	1
454	1	42	ARG	HB2	H	1.657	1
455	1	42	ARG	HG2	H	1.486	1
456	1	42	ARG	HD2	H	3.059	1
457	1	42	ARG	C	C	173.790	1
458	1	42	ARG	CA	C	55.192	1
459	1	42	ARG	CB	C	31.584	1
460	1	42	ARG	CG	C	27.112	1
461	1	42	ARG	CD	C	43.650	1
462	1	42	ARG	N	N	123.245	1
463	1	43	LEU	H	H	8.770	1
464	1	43	LEU	HA	H	5.336	1
465	1	43	LEU	HB2	H	1.523	1
466	1	43	LEU	HB3	H	1.142	1
467	1	43	LEU	HD11	H	0.770	1
468	1	43	LEU	HD12	H	0.770	1
469	1	43	LEU	HD13	H	0.770	1
470	1	43	LEU	HD21	H	0.741	1
471	1	43	LEU	HD22	H	0.741	1
472	1	43	LEU	HD23	H	0.741	1
473	1	43	LEU	C	C	175.145	1
474	1	43	LEU	CA	C	53.028	1
475	1	43	LEU	CB	C	45.435	1
476	1	43	LEU	CD1	C	24.139	1
477	1	43	LEU	CD2	C	26.500	1
478	1	43	LEU	N	N	124.647	1
479	1	44	ILE	H	H	9.270	1
480	1	44	ILE	HA	H	4.896	1
481	1	44	ILE	HB	H	1.736	1
482	1	44	ILE	HG12	H	1.049	1
483	1	44	ILE	HG13	H	1.327	1
484	1	44	ILE	HG21	H	0.660	1
485	1	44	ILE	HG22	H	0.660	1
486	1	44	ILE	HG23	H	0.660	1
487	1	44	ILE	HD11	H	0.624	1
488	1	44	ILE	HD12	H	0.624	1
489	1	44	ILE	HD13	H	0.624	1
490	1	44	ILE	C	C	175.469	1
491	1	44	ILE	CA	C	58.902	1
492	1	44	ILE	CB	C	40.898	1
493	1	44	ILE	CG1	C	27.742	1
494	1	44	ILE	CG2	C	17.543	1
495	1	44	ILE	CD1	C	12.631	1
496	1	44	ILE	N	N	123.080	1
497	1	45	PHE	H	H	8.847	1
498	1	45	PHE	HA	H	5.081	1
499	1	45	PHE	HB2	H	2.787	1
500	1	45	PHE	HB3	H	2.953	1
501	1	45	PHE	HD1	H	7.296	1
502	1	45	PHE	HD2	H	7.296	1
503	1	45	PHE	HE1	H	7.590	1
504	1	45	PHE	HE2	H	7.590	1
505	1	45	PHE	CA	C	56.980	1
506	1	45	PHE	CB	C	43.755	1
507	1	45	PHE	N	N	125.374	1
508	1	46	ALA	H	H	8.979	1
509	1	46	ALA	HA	H	3.666	1
510	1	46	ALA	HB1	H	0.920	1
511	1	46	ALA	HB2	H	0.920	1
512	1	46	ALA	HB3	H	0.920	1
513	1	46	ALA	C	C	177.400	1
514	1	46	ALA	CA	C	52.582	1
515	1	46	ALA	CB	C	16.591	1
516	1	46	ALA	N	N	133.050	1
517	1	47	GLY	H	H	8.187	1
518	1	47	GLY	HA2	H	3.399	1
519	1	47	GLY	HA3	H	4.054	1
520	1	47	GLY	C	C	173.731	1
521	1	47	GLY	CA	C	45.383	1
522	1	47	GLY	N	N	102.540	1
523	1	48	LYS	H	H	8.015	1
524	1	48	LYS	HA	H	4.593	1
525	1	48	LYS	HB2	H	1.869	1
526	1	48	LYS	HB3	H	1.869	1
527	1	48	LYS	HG2	H	1.492	1
528	1	48	LYS	HG3	H	1.487	1
529	1	48	LYS	C	C	174.545	1
530	1	48	LYS	CA	C	54.500	1
531	1	48	LYS	CB	C	34.504	1
532	1	48	LYS	CG	C	24.592	1
533	1	48	LYS	CD	C	29.331	1
534	1	48	LYS	CE	C	42.260	1
535	1	48	LYS	N	N	122.108	1
536	1	49	GLN	H	H	8.641	1
537	1	49	GLN	HA	H	4.532	1
538	1	49	GLN	HB2	H	1.951	1
539	1	49	GLN	HG2	H	2.230	1
540	1	49	GLN	C	C	175.782	1
541	1	49	GLN	CA	C	55.871	1
542	1	49	GLN	CB	C	29.028	1
543	1	49	GLN	CG	C	34.709	1
544	1	49	GLN	N	N	122.718	1
545	1	50	LEU	H	H	8.643	1
546	1	50	LEU	HA	H	4.072	1
547	1	50	LEU	HB2	H	1.019	1
548	1	50	LEU	HB3	H	1.467	1
549	1	50	LEU	HD11	H	0.487	1
550	1	50	LEU	HD12	H	0.487	1
551	1	50	LEU	HD13	H	0.487	1
552	1	50	LEU	C	C	176.732	1
553	1	50	LEU	CA	C	54.374	1
554	1	50	LEU	CB	C	41.603	1
555	1	50	LEU	CD1	C	19.564	1
556	1	50	LEU	CD2	C	25.858	1
557	1	50	LEU	N	N	126.073	1
558	1	51	GLU	H	H	8.390	1
559	1	51	GLU	HA	H	4.475	1
560	1	51	GLU	HB2	H	1.891	1
561	1	51	GLU	HG2	H	2.301	1
562	1	51	GLU	C	C	175.611	1
563	1	51	GLU	CA	C	55.955	1
564	1	51	GLU	CB	C	31.858	1
565	1	51	GLU	CG	C	36.566	1
566	1	51	GLU	N	N	123.151	1
567	1	52	ASP	H	H	8.212	1
568	1	52	ASP	HA	H	4.450	1
569	1	52	ASP	HB2	H	2.495	1
570	1	52	ASP	CA	C	55.954	1
571	1	52	ASP	CB	C	40.604	1
572	1	52	ASP	N	N	120.912	1
573	1	53	GLY	HA2	H	4.028	1
574	1	53	GLY	HA3	H	4.028	1
575	1	53	GLY	C	C	174.772	1
576	1	53	GLY	CA	C	45.179	1
577	1	54	ARG	H	H	7.470	1
578	1	54	ARG	HA	H	4.684	1
579	1	54	ARG	HB2	H	2.136	1
580	1	54	ARG	HB3	H	2.136	1
581	1	54	ARG	HG2	H	1.684	1
582	1	54	ARG	HG3	H	1.684	1
583	1	54	ARG	HD2	H	3.082	1
584	1	54	ARG	HD3	H	3.082	1
585	1	54	ARG	C	C	175.160	1
586	1	54	ARG	CA	C	54.372	1
587	1	54	ARG	CB	C	32.489	1
588	1	54	ARG	CG	C	27.587	1
589	1	54	ARG	CD	C	42.955	1
590	1	54	ARG	N	N	119.526	1
591	1	55	THR	H	H	8.847	1
592	1	55	THR	HA	H	5.229	1
593	1	55	THR	HB	H	4.512	1
594	1	55	THR	HG21	H	1.088	1
595	1	55	THR	HG22	H	1.088	1
596	1	55	THR	HG23	H	1.088	1
597	1	55	THR	C	C	176.429	1
598	1	55	THR	CA	C	59.706	1
599	1	55	THR	CB	C	72.408	1
600	1	55	THR	CG2	C	22.385	1
601	1	55	THR	N	N	109.009	1
602	1	56	LEU	H	H	8.105	1
603	1	56	LEU	HA	H	4.043	1
604	1	56	LEU	HB2	H	2.068	1
605	1	56	LEU	HB3	H	1.203	1
606	1	56	LEU	HD11	H	0.593	1
607	1	56	LEU	HD12	H	0.593	1
608	1	56	LEU	HD13	H	0.593	1
609	1	56	LEU	HD21	H	1.631	1
610	1	56	LEU	HD22	H	1.631	1
611	1	56	LEU	HD23	H	1.631	1
612	1	56	LEU	C	C	180.637	1
613	1	56	LEU	CA	C	58.714	1
614	1	56	LEU	CB	C	40.601	1
615	1	56	LEU	CD1	C	23.402	1
616	1	56	LEU	CD2	C	27.097	1
617	1	56	LEU	N	N	118.322	1
618	1	57	SER	H	H	8.493	1
619	1	57	SER	HA	H	4.246	1
620	1	57	SER	HB2	H	3.781	1
621	1	57	SER	HB3	H	3.781	1
622	1	57	SER	C	C	178.242	1
623	1	57	SER	CA	C	61.068	1
624	1	57	SER	CB	C	62.653	1
625	1	57	SER	N	N	113.282	1
626	1	58	ASP	H	H	7.969	1
627	1	58	ASP	HA	H	4.241	1
628	1	58	ASP	HB2	H	2.964	1
629	1	58	ASP	HB3	H	2.243	1
630	1	58	ASP	C	C	177.414	1
631	1	58	ASP	CA	C	57.413	1
632	1	58	ASP	CB	C	40.404	1
633	1	58	ASP	N	N	124.760	1
634	1	59	TYR	H	H	7.264	1
635	1	59	TYR	HA	H	4.580	1
636	1	59	TYR	HB2	H	2.476	1
637	1	59	TYR	HB3	H	3.364	1
638	1	59	TYR	HD1	H	7.249	1
639	1	59	TYR	HD2	H	7.249	1
640	1	59	TYR	HE1	H	6.844	1
641	1	59	TYR	HE2	H	6.844	1
642	1	59	TYR	C	C	174.448	1
643	1	59	TYR	CA	C	58.460	1
644	1	59	TYR	CB	C	39.784	1
645	1	59	TYR	CE2	C	118.539	1
646	1	59	TYR	N	N	115.990	1
647	1	60	ASN	H	H	8.119	1
648	1	60	ASN	HA	H	4.307	1
649	1	60	ASN	HB2	H	2.734	1
650	1	60	ASN	HB3	H	3.267	1
651	1	60	ASN	C	C	174.073	1
652	1	60	ASN	CA	C	54.097	1
653	1	60	ASN	CB	C	37.434	1
654	1	60	ASN	N	N	116.071	1
655	1	61	ILE	H	H	7.130	1
656	1	61	ILE	HA	H	3.407	1
657	1	61	ILE	HB	H	1.215	1
658	1	61	ILE	HG12	H	0.916	1
659	1	61	ILE	HG21	H	0.435	1
660	1	61	ILE	HG22	H	0.435	1
661	1	61	ILE	HG23	H	0.435	1
662	1	61	ILE	HD11	H	0.301	1
663	1	61	ILE	HD12	H	0.301	1
664	1	61	ILE	HD13	H	0.301	1
665	1	61	ILE	C	C	174.639	1
666	1	61	ILE	CA	C	62.391	1
667	1	61	ILE	CB	C	37.235	1
668	1	61	ILE	CG1	C	27.497	1
669	1	61	ILE	CG2	C	17.085	1
670	1	61	ILE	CD1	C	14.523	1
671	1	61	ILE	N	N	119.552	1
672	1	62	GLN	H	H	8.136	1
673	1	62	GLN	HA	H	4.345	1
674	1	62	GLN	HB2	H	2.217	1
675	1	62	GLN	HG2	H	2.388	1
676	1	62	GLN	C	C	174.219	1
677	1	62	GLN	CA	C	53.800	1
678	1	62	GLN	CB	C	31.253	1
679	1	62	GLN	CG	C	34.163	1
680	1	62	GLN	N	N	126.003	1
681	1	63	LYS	H	H	7.985	1
682	1	63	LYS	HA	H	3.923	1
683	1	63	LYS	HB2	H	1.895	1
684	1	63	LYS	HB3	H	1.895	1
685	1	63	LYS	HG2	H	1.468	1
686	1	63	LYS	HG3	H	1.468	1
687	1	63	LYS	C	C	176.153	1
688	1	63	LYS	CA	C	57.474	1
689	1	63	LYS	CB	C	32.763	1
690	1	63	LYS	CG	C	23.970	1
691	1	63	LYS	CD	C	30.091	1
692	1	63	LYS	CE	C	42.430	1
693	1	63	LYS	N	N	117.392	1
694	1	64	GLU	H	H	9.296	1
695	1	64	GLU	HA	H	3.354	1
696	1	64	GLU	HB2	H	2.232	1
697	1	64	GLU	HB3	H	2.232	1
698	1	64	GLU	HG2	H	2.469	1
699	1	64	GLU	HG3	H	2.469	1
700	1	64	GLU	C	C	174.555	1
701	1	64	GLU	CA	C	58.304	1
702	1	64	GLU	CB	C	26.426	1
703	1	64	GLU	CG	C	37.509	1
704	1	64	GLU	N	N	115.431	1
705	1	65	SEP	H	H	7.843	1
706	1	65	SEP	C	C	172.110	1
707	1	65	SEP	N	N	114.604	1
708	1	65	SEP	CA	C	57.990	1
709	1	65	SEP	CB	C	66.808	1
710	1	65	SEP	HA	H	4.216	1
711	1	66	THR	H	H	8.503	1
712	1	66	THR	HA	H	5.345	1
713	1	66	THR	HB	H	4.004	1
714	1	66	THR	HG21	H	0.864	1
715	1	66	THR	HG22	H	0.864	1
716	1	66	THR	HG23	H	0.864	1
717	1	66	THR	C	C	173.888	1
718	1	66	THR	CA	C	62.699	1
719	1	66	THR	CB	C	71.260	1
720	1	66	THR	CG2	C	21.871	1
721	1	66	THR	N	N	116.035	1
722	1	67	LEU	H	H	9.340	1
723	1	67	LEU	HA	H	5.043	1
724	1	67	LEU	HB2	H	1.642	1
725	1	67	LEU	HB3	H	1.642	1
726	1	67	LEU	HD11	H	0.674	1
727	1	67	LEU	HD12	H	0.674	1
728	1	67	LEU	HD13	H	0.674	1
729	1	67	LEU	C	C	174.973	1
730	1	67	LEU	CA	C	53.749	1
731	1	67	LEU	CB	C	44.830	1
732	1	67	LEU	CD1	C	28.890	1
733	1	67	LEU	CD2	C	25.412	1
734	1	67	LEU	N	N	126.585	1
735	1	68	HIS	H	H	9.151	1
736	1	68	HIS	HA	H	5.110	1
737	1	68	HIS	HB2	H	2.871	1
738	1	68	HIS	HB3	H	2.980	1
739	1	68	HIS	C	C	173.909	1
740	1	68	HIS	CA	C	56.325	1
741	1	68	HIS	CB	C	32.695	1
742	1	68	HIS	N	N	120.018	1
743	1	69	LEU	H	H	8.348	1
744	1	69	LEU	HA	H	5.144	1
745	1	69	LEU	HB2	H	1.565	1
746	1	69	LEU	HB3	H	1.079	1
747	1	69	LEU	HD11	H	0.713	1
748	1	69	LEU	HD12	H	0.713	1
749	1	69	LEU	HD13	H	0.713	1
750	1	69	LEU	HD21	H	1.094	1
751	1	69	LEU	HD22	H	1.094	1
752	1	69	LEU	HD23	H	1.094	1
753	1	69	LEU	C	C	175.301	1
754	1	69	LEU	CA	C	53.724	1
755	1	69	LEU	CB	C	44.396	1
756	1	69	LEU	CD1	C	26.115	1
757	1	69	LEU	N	N	123.969	1
758	1	70	VAL	H	H	9.204	1
759	1	70	VAL	HA	H	4.341	1
760	1	70	VAL	HB	H	1.998	1
761	1	70	VAL	HG11	H	0.868	1
762	1	70	VAL	HG12	H	0.868	1
763	1	70	VAL	HG13	H	0.868	1
764	1	70	VAL	HG21	H	0.868	1
765	1	70	VAL	HG22	H	0.868	1
766	1	70	VAL	HG23	H	0.868	1
767	1	70	VAL	C	C	173.982	1
768	1	70	VAL	CA	C	60.746	1
769	1	70	VAL	CB	C	34.718	1
770	1	70	VAL	CG1	C	21.264	1
771	1	70	VAL	CG2	C	20.956	1
772	1	70	VAL	N	N	127.206	1
773	1	71	LEU	H	H	8.143	1
774	1	71	LEU	HA	H	5.011	1
775	1	71	LEU	HB2	H	1.545	1
776	1	71	LEU	HB3	H	1.655	1
777	1	71	LEU	HD11	H	0.714	1
778	1	71	LEU	HD12	H	0.714	1
779	1	71	LEU	HD13	H	0.714	1
780	1	71	LEU	HD21	H	0.868	1
781	1	71	LEU	HD22	H	0.868	1
782	1	71	LEU	HD23	H	0.868	1
783	1	71	LEU	C	C	177.896	1
784	1	71	LEU	CA	C	53.839	1
785	1	71	LEU	CB	C	42.737	1
786	1	71	LEU	N	N	123.453	1
787	1	72	ARG	H	H	8.626	1
788	1	72	ARG	HA	H	4.252	1
789	1	72	ARG	HB2	H	1.750	1
790	1	72	ARG	HG2	H	1.512	1
791	1	72	ARG	HD2	H	3.117	1
792	1	72	ARG	HD3	H	3.117	1
793	1	72	ARG	C	C	175.317	1
794	1	72	ARG	CA	C	55.702	1
795	1	72	ARG	CB	C	31.368	1
796	1	72	ARG	CG	C	27.216	1
797	1	72	ARG	CD	C	43.499	1
798	1	72	ARG	N	N	123.759	1
799	1	73	LEU	H	H	8.378	1
800	1	73	LEU	HA	H	4.374	1
801	1	73	LEU	C	C	177.383	1
802	1	73	LEU	CA	C	54.904	1
803	1	73	LEU	CB	C	42.397	1
804	1	73	LEU	N	N	124.618	1
805	1	74	ARG	H	H	8.444	1
806	1	74	ARG	C	C	174.784	1
807	1	74	ARG	N	N	122.091	1
808	1	75	GLY	H	H	7.893	1
809	1	75	GLY	C	C	173.514	1
810	1	75	GLY	CA	C	45.213	1
811	1	75	GLY	N	N	114.939	1
812	1	76	GLY	H	H	7.922	1
813	1	76	GLY	CA	C	46.074	1

Release date

2022-06-29

Citation

Kinetic Constraints in the Specific Interaction between Phosphorylated Ubiquitin and Proteasomal Shuttle Factors
Qin, L.Y., Gong, Z., Liu, K., Dong, X., Tang, C.
Biomolecules (2021), 11, 1008-1008, PubMed 34356632 , DOI 10.3390/biom11071008 , Abstract

Related entities 1. Polyubiquitin-B, Ubiquilin-2, entity 1, : 138 : 183 entities Detail

Related entities 2. Polyubiquitin-B, Ubiquilin-2, entity 2, : 1 : 5 : 102 entities Detail

Interaction partners 2. Polyubiquitin-B, Ubiquilin-2, entity 2, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 3 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	Polyubiquitin-B	[U-100% 12C; U-100% 14N]	protein	0.75 mM
2	Ubiquilin-2	[U-100% 13C; U-100% 15N]	protein	0.5 mM
3	HEPES	natural abundance	buffer	20 mM
4	sodium chloride	natural abundance	salt	0.15 M
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

#	Name	Isotope labeling	Type	Concentration
1	Polyubiquitin-B	[U-100% 12C; U-100% 14N]	protein	0.75 mM
2	Ubiquilin-2	[U-100% 13C; U-100% 15N]	protein	0.5 mM
3	HEPES	natural abundance	buffer	20 mM
4	sodium chloride	natural abundance	salt	0.15 M
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

#	Name	Isotope labeling	Type	Concentration
1	Polyubiquitin-B	[U-100% 12C; U-100% 14N]	protein	0.75 mM
2	Ubiquilin-2	[U-100% 13C; U-100% 15N]	protein	0.5 mM
3	HEPES	natural abundance	buffer	20 mM
4	sodium chloride	natural abundance	salt	0.15 M
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36427_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:64:GLU:C	1:65:SEP:N	unknown	unknown	n/a
1:65:SEP:C	1:66:THR:N	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
1	65	SEP	PHOSPHOSERINE	Assigned chemical shifts

Content subtype: bmr36427_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	813		98.7 (chain: 1, length: 76)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 76, Sequence coverage: 98.7%
- Total number of assignments
  - ¹H chemical shifts: 449
  - ¹³C chemical shifts: 295
  - ¹⁵N chemical shifts: 69
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
65	SEP	C	172.11

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	478	363	75.9
¹³C chemical shifts	349	294	84.2
¹⁵N chemical shifts	85	69	81.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	155	145	93.5
¹³C chemical shifts	151	139	92.1
¹⁵N chemical shifts	73	69	94.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	323	218	67.5
¹³C chemical shifts	198	155	78.3
¹⁵N chemical shifts	12	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	43	86.0
¹³C chemical shifts	50	42	84.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	16	10	62.5
¹³C chemical shifts	16	2	12.5

Keywords PROTEIN BINDING, protein-protein interaction; phosphorylation; proteasomal shuttle factor; ubiquitin-associated domain; induced fit

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Found 1 Document (2.259 seconds)

BMRB: 36427

Related entities 1. Polyubiquitin-B, Ubiquilin-2, entity 1, : 138 : 183 entities Detail

Related entities 2. Polyubiquitin-B, Ubiquilin-2, entity 2, : 1 : 5 : 102 entities Detail

Interaction partners 2. Polyubiquitin-B, Ubiquilin-2, entity 2, : 4 interactors Detail

Experiments performed 3 experiments Detail

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Chemical shift validation 4 contents Detail