BMRBj - BMREntryMaster

Found 1 Document (0.586 seconds)

BMRB: 36430

1X4D

Solution structure of The first RNA binding domain of Matrin-3

Authors

Muto, Y., Kobayashi, N., Yokoyama, S., RIKEN Structural Genomics/Proteomics Initiative (RSGI), R.

Assembly

Matrin-3

Entity

1. Matrin-3 (polymer, Thiol state: not present), 102 monomers, 11334.83 Da Detail

GSSGSSGQKG RVETRRVVHI MDFQRGKNLR YQLLQLVEPF GVISNHLILN KINEAFIEMA TTEDAQAAVD YYTTTPALVF GKPVRVHLSQ KYKRIKSGPS SG

Formula weight

11334.83 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 90.3 %, Completeness (bb): 86.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.3 % (1085 of 1201)	91.0 % (567 of 623)	92.0 % (426 of 463)	80.0 % (92 of 115)
Backbone	86.8 % (524 of 604)	84.7 % (177 of 209)	89.2 % (265 of 297)	83.7 % (82 of 98)
Sidechain	93.9 % (648 of 690)	94.2 % (390 of 414)	95.8 % (248 of 259)	58.8 % (10 of 17)
Aromatic	91.7 % (77 of 84)	95.2 % (40 of 42)	88.1 % (37 of 42)
Methyl	100.0 % (116 of 116)	100.0 % (58 of 58)	100.0 % (58 of 58)

1. Matrin-3

GSSGSSGQKG RVETRRVVHI MDFQRGKNLR YQLLQLVEPF GVISNHLILN KINEAFIEMA TTEDAQAAVD YYTTTPALVF GKPVRVHLSQ KYKRIKSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1.0 mM [U-99% 13C; U-99% 15N] RNA binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA binding protein	[U-99% 13C; U-99% 15N]	protein	1.0 mM
2	Tris-Hcl	[U-100% 2H]	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

Release date

2021-08-17

Citation

Solution structure of The first RNA binding domain of Matrin-3
He, F., Kuwasako, K., Takizawa, M., Takahashi, M., Tsuda, K., Nagata, T., Watanabe, S., Tanaka, A., Kobayashi, N., Kigawa, T., Guntert, P., Shirouzu, M., Yokoyama, S., Muto, Y.

Related entities 1. Matrin-3, : 1 : 1 : 6 : 77 entities Detail

Interaction partners 1. Matrin-3, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36430_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36430_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1210		91.2 (chain: 1, length: 102)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 102, Sequence coverage: 91.2%
- Total number of assignments
  - ¹H chemical shifts: 696
  - ¹³C chemical shifts: 425
  - ¹⁵N chemical shifts: 89
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
62	THR	HG1	5.617
73	THR	HG1	5.696
75	THR	HG1	5.375

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	623	573	92.0
¹³C chemical shifts	463	425	91.8
¹⁵N chemical shifts	115	89	77.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	209	180	86.1
¹³C chemical shifts	204	177	86.8
¹⁵N chemical shifts	98	79	80.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	414	393	94.9
¹³C chemical shifts	259	248	95.8
¹⁵N chemical shifts	17	10	58.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	60	100.0
¹³C chemical shifts	60	60	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	40	95.2
¹³C chemical shifts	42	37	88.1

Keywords ALS/FTD, PSI-2, Protein Structure Initiative, RIKEN Structural Genomics/Proteomics Initiative, RNA BINDING PROTEIN, RRM, RSGI, Structural Genomics, nuclear matrix protein

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Found 1 Document (0.586 seconds)

BMRB: 36430

Sample

Related entities 1. Matrin-3, : 1 : 1 : 6 : 77 entities Detail

Interaction partners 1. Matrin-3, : 3 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail