BMRBj - BMREntryMaster

Found 1 Document (6.416 seconds)

BMRB: 36431

1X4F

The solution structure of the second RRM domain of Matrin-3

Authors

Muto, Y., Kobayashi, N., Yokoyama, S., RIKEN Structural Genomics/Proteomics Initiative (RSGI), R.

Assembly

Matrin-3

Entity

1. Matrin-3 (polymer, Thiol state: all free), 112 monomers, 12477.28 Da Detail

GSSGSSGKKP EGKPDQKFDQ KQELGRVIHL SNLPHSGYSD SAVLKLAEPY GKIKNYILMR MKSQAFIEME TREDAMAMVD HCLKKALWFQ GRCVKVDLSE KYKKLVSGPS SG

Formula weight

12477.28 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.0 %, Completeness: 89.2 %, Completeness (bb): 88.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.2 % (1185 of 1328)	88.9 % (623 of 701)	91.1 % (463 of 508)	83.2 % (99 of 119)
Backbone	88.4 % (585 of 662)	87.3 % (200 of 229)	89.9 % (293 of 326)	86.0 % (92 of 107)
Sidechain	90.4 % (694 of 768)	89.6 % (423 of 472)	93.0 % (264 of 284)	58.3 % (7 of 12)
Aromatic	97.7 % (84 of 86)	97.7 % (42 of 43)	97.6 % (41 of 42)	100.0 % (1 of 1)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. Matrin-3

GSSGSSGKKP EGKPDQKFDQ KQELGRVIHL SNLPHSGYSD SAVLKLAEPY GKIKNYILMR MKSQAFIEME TREDAMAMVD HCLKKALWFQ GRCVKVDLSE KYKKLVSGPS SG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 1 mM [U-99% 13C; U-99% 15N] RNA binding protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RNA binding protein	[U-99% 13C; U-99% 15N]	protein	1 mM
2	Tris-Hcl	[U-100% 2H]	buffer	20 mM
3	NaCl	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

Release date

2021-08-17

Citation

¹H, ¹³C and ¹⁵N resonance assignments and solution structures of the two RRM domains of Matrin-3
He, F., Kuwasako, K., Takizawa, M., Takahashi, M., Tsuda, K., Nagata, T., Watanabe, S., Tanaka, A., Kobayashi, N., Kigawa, T., Guntert, P., Shirouzu, M., Yokoyama, S., Muto, Y.
Biomol. NMR Assign. (2022), 16, 41-49, PubMed 34783967 , DOI 10.1007/s12104-021-10057-0 , Abstract

Related entities 1. Matrin-3, : 1 : 1 : 4 : 31 entities Detail

Interaction partners 1. Matrin-3, : 94 interactors Detail

More details for 94 interactors identified by 44 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36431_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36431_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1278		90.2 (chain: 1, length: 112)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 112, Sequence coverage: 90.2%
- Total number of assignments
  - ¹H chemical shifts: 723
  - ¹³C chemical shifts: 459
  - ¹⁵N chemical shifts: 96
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	701	619	88.3
¹³C chemical shifts	508	459	90.4
¹⁵N chemical shifts	119	96	80.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	229	196	85.6
¹³C chemical shifts	224	195	87.1
¹⁵N chemical shifts	107	89	83.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	472	423	89.6
¹³C chemical shifts	284	264	93.0
¹⁵N chemical shifts	12	7	58.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	52	100.0
¹³C chemical shifts	52	52	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	42	97.7
¹³C chemical shifts	42	41	97.6
¹⁵N chemical shifts	1	1	100.0

Keywords ALS/FTD, PSI-2, Protein Structure Initiative, RIKEN Structural Genomics/Proteomics Initiative, RNA BINDING PROTEIN, RRM, RSGI, Structural Genomics, nuclear matrix protein

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Found 1 Document (6.416 seconds)

BMRB: 36431

Sample

Related entities 1. Matrin-3, : 1 : 1 : 4 : 31 entities Detail

Interaction partners 1. Matrin-3, : 94 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail