BMRBj - BMREntryMaster

Found 1 Document (0.601 seconds)

BMRB: 36527

8HPB

NMR Structure of OsCIE1-Ubox S237D mutant

Authors

Zhang, Y., Yu, C.Z., Lan, W.X.

Assembly

U-box domain-containing protein 12 (E.C.2.3.2.27)

Entity

1. U-box domain-containing protein 12 (E.C.2.3.2.27) (polymer, Thiol state: all free), 80 monomers, 8972.215 × 2 Da Detail

GAMIIPDEFR CPIDLELMQD PVIVSSGQTY ERSCIQKWLD SGHKTCPKTQ QPLSHTSLTP NFVLKSLISQ WCEANGIELP

Total weight

17944.43 Da

Max. entity weight

8972.215 Da

Source organism

Oryza sativa Japonica Group

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 523, ¹³C: 286, ¹⁵N: 69 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Ambig. code
1	1	2	ALA	CA	C	52.463	1
2	1	2	ALA	CB	C	19.536	1
3	1	2	ALA	C	C	177.34	1
4	1	3	MET	CA	C	55.409	1
5	1	3	MET	CB	C	33.304	1
6	1	3	MET	C	C	175.523	1
7	1	3	MET	HA	H	4.566	1
8	1	3	MET	HB2	H	1.547
9	1	3	MET	HB3	H	2.075
10	1	3	MET	HG2	H	2.591
11	1	3	MET	HG3	H	2.591
12	1	3	MET	H	H	8.419	1
13	1	3	MET	N	N	120.376	1
14	1	4	ILE	CA	C	60.604	1
15	1	4	ILE	CB	C	38.42	1
16	1	4	ILE	CD1	C	12.508	1
17	1	4	ILE	CG1	C	27.843	1
18	1	4	ILE	CG2	C	17.374	1
19	1	4	ILE	C	C	175.517	1
20	1	4	ILE	HA	H	4.159	1
21	1	4	ILE	HB	H	1.837	1
22	1	4	ILE	HD11	H	0.723	1
23	1	4	ILE	HD12	H	0.723	1
24	1	4	ILE	HD13	H	0.723	1
25	1	4	ILE	HG13	H	1.178
26	1	4	ILE	HG12	H	1.505
27	1	4	ILE	HG21	H	0.733	1
28	1	4	ILE	HG22	H	0.733	1
29	1	4	ILE	HG23	H	0.733	1
30	1	4	ILE	H	H	8.389	1
31	1	4	ILE	N	N	125.097	1
32	1	5	ILE	CA	C	58.45	1
33	1	5	ILE	CB	C	39.63	1
34	1	5	ILE	CD1	C	13.416	1
35	1	5	ILE	CG1	C	27.407	1
36	1	5	ILE	CG2	C	17.484	1
37	1	5	ILE	HA	H	4.143	1
38	1	5	ILE	HB	H	1.539	1
39	1	5	ILE	HD11	H	0.854	1
40	1	5	ILE	HD12	H	0.854	1
41	1	5	ILE	HD13	H	0.854	1
42	1	5	ILE	HG12	H	1.186
43	1	5	ILE	HG13	H	1.186
44	1	5	ILE	HG21	H	0.652	1
45	1	5	ILE	HG22	H	0.652	1
46	1	5	ILE	HG23	H	0.652	1
47	1	5	ILE	H	H	8.22	1
48	1	5	ILE	N	N	127.662	1
49	1	6	PRO	CA	C	62.861	1
50	1	6	PRO	CB	C	31.227	1
51	1	6	PRO	CD	C	50.863	1
52	1	6	PRO	C	C	177.204	1
53	1	6	PRO	HA	H	3.975	1
54	1	6	PRO	HB2	H	1.761
55	1	6	PRO	HB3	H	1.761
56	1	6	PRO	HG2	H	2.115
57	1	6	PRO	HG3	H	2.115
58	1	6	PRO	HD2	H	3.498
59	1	6	PRO	HD3	H	3.498
60	1	7	ASP	CA	C	58.058	1
61	1	7	ASP	CB	C	40.663	1
62	1	7	ASP	C	C	178.415	1
63	1	7	ASP	HA	H	3.914	1
64	1	7	ASP	HB2	H	2.382
65	1	7	ASP	HB3	H	2.382
66	1	7	ASP	H	H	8.591	1
67	1	7	ASP	N	N	125.186	1
68	1	8	GLU	CA	C	60.206	1
69	1	8	GLU	CB	C	28.57	1
70	1	8	GLU	CG	C	32.14	1
71	1	8	GLU	C	C	176.862	1
72	1	8	GLU	HA	H	3.884	1
73	1	8	GLU	HB3	H	1.576
74	1	8	GLU	HB2	H	1.73
75	1	8	GLU	HG2	H	2.2
76	1	8	GLU	HG3	H	2.2
77	1	8	GLU	H	H	9.189	1
78	1	8	GLU	N	N	116.179	1
79	1	9	PHE	CA	C	53.17	1
80	1	9	PHE	CB	C	37.139	1
81	1	9	PHE	CD1	C	123.7
82	1	9	PHE	CD2	C	123.7
83	1	9	PHE	CE1	C	126.031
84	1	9	PHE	CE2	C	126.031
85	1	9	PHE	C	C	174.547	1
86	1	9	PHE	CZ	C	123.7	1
87	1	9	PHE	HA	H	4.618	1
88	1	9	PHE	HB2	H	2.679
89	1	9	PHE	HB3	H	3.279
90	1	9	PHE	HD2	H	6.296
91	1	9	PHE	HD1	H	6.296
92	1	9	PHE	HE1	H	6.871
93	1	9	PHE	HE2	H	6.871
94	1	9	PHE	H	H	7.431	1
95	1	9	PHE	HZ	H	7.015	1
96	1	9	PHE	N	N	114.522	1
97	1	10	ARG	CA	C	54.636	1
98	1	10	ARG	CB	C	32.45	1
99	1	10	ARG	CD	C	43.68	1
100	1	10	ARG	C	C	175.308	1
101	1	10	ARG	HA	H	4.479	1
102	1	10	ARG	HB2	H	1.589
103	1	10	ARG	HB3	H	1.589
104	1	10	ARG	HD2	H	3.064
105	1	10	ARG	HD3	H	3.064
106	1	10	ARG	HG2	H	1.02
107	1	10	ARG	HG3	H	1.02
108	1	10	ARG	H	H	7.431	1
109	1	10	ARG	N	N	118.614	1
110	1	11	CYS	CA	C	54.42	1
111	1	11	CYS	CB	C	30.953	1
112	1	11	CYS	HA	H	4.607	1
113	1	11	CYS	HB3	H	2.925
114	1	11	CYS	HB2	H	3.417
115	1	11	CYS	H	H	8.512	1
116	1	11	CYS	N	N	131.333	1
117	1	12	PRO	CA	C	64.512	1
118	1	12	PRO	CB	C	33.184	1
119	1	12	PRO	CD	C	52.54	1
120	1	12	PRO	HA	H	4.307	1
121	1	12	PRO	HB2	H	1.754
122	1	12	PRO	HB3	H	1.754
123	1	12	PRO	HD3	H	4.048
124	1	12	PRO	HD2	H	4.161
125	1	12	PRO	HG2	H	2.344
126	1	12	PRO	HG3	H	2.344
127	1	12	PRO	C	C	178.101	1
128	1	13	ILE	CA	C	64.656	1
129	1	13	ILE	CB	C	37.393	1
130	1	13	ILE	CD1	C	13.264	1
131	1	13	ILE	CG2	C	16.66	1
132	1	13	ILE	CG1	C	26.729	1
133	1	13	ILE	C	C	177.142	1
134	1	13	ILE	HA	H	3.893	1
135	1	13	ILE	HB	H	2.379	1
136	1	13	ILE	HD11	H	0.22	1
137	1	13	ILE	HD12	H	0.22	1
138	1	13	ILE	HD13	H	0.22	1
139	1	13	ILE	HG13	H	1.554
140	1	13	ILE	HG12	H	1.554
141	1	13	ILE	HG21	H	0.821	1
142	1	13	ILE	HG22	H	0.821	1
143	1	13	ILE	HG23	H	0.821	1
144	1	13	ILE	H	H	11.274	1
145	1	13	ILE	N	N	122.569	1
146	1	14	ASP	CA	C	53.517	1
147	1	14	ASP	CB	C	41.546	1
148	1	14	ASP	C	C	176.807	1
149	1	14	ASP	HA	H	4.743	1
150	1	14	ASP	HB3	H	2.597
151	1	14	ASP	HB2	H	3.062
152	1	14	ASP	H	H	8.33	1
153	1	14	ASP	N	N	118.959	1
154	1	15	LEU	CA	C	56.478	1
155	1	15	LEU	CB	C	38.44	1
156	1	15	LEU	CD1	C	23
157	1	15	LEU	CD2	C	22.9106
158	1	15	LEU	C	C	176.06	1
159	1	15	LEU	HA	H	4.063	1
160	1	15	LEU	HB2	H	1.609
161	1	15	LEU	HB3	H	2.045
162	1	15	LEU	HD11	H	0.766
163	1	15	LEU	HD12	H	0.766
164	1	15	LEU	HD13	H	0.766
165	1	15	LEU	HD21	H	1.12
166	1	15	LEU	HD22	H	1.12
167	1	15	LEU	HD23	H	1.12
168	1	15	LEU	HG	H	1.398	1
169	1	15	LEU	H	H	8.686	1
170	1	15	LEU	N	N	116.03	1
171	1	16	GLU	CA	C	54.553	1
172	1	16	GLU	CB	C	31.292	1
173	1	16	GLU	C	C	177.457	1
174	1	16	GLU	HA	H	4.537	1
175	1	16	GLU	HB2	H	1.935
176	1	16	GLU	HB3	H	1.935
177	1	16	GLU	HG2	H	2.264
178	1	16	GLU	HG3	H	2.264
179	1	16	GLU	H	H	7.949	1
180	1	16	GLU	N	N	118.745	1
181	1	17	LEU	CA	C	56.194	1
182	1	17	LEU	CB	C	42.959	1
183	1	17	LEU	CD1	C	22.9
184	1	17	LEU	CD2	C	23.56
185	1	17	LEU	C	C	176.725	1
186	1	17	LEU	HA	H	4.048	1
187	1	17	LEU	HB3	H	1.44
188	1	17	LEU	HB2	H	1.679
189	1	17	LEU	HD11	H	0.648
190	1	17	LEU	HD12	H	0.648
191	1	17	LEU	HD13	H	0.648
192	1	17	LEU	HD21	H	0.925
193	1	17	LEU	HD22	H	0.925
194	1	17	LEU	HD23	H	0.925
195	1	17	LEU	HG	H	1.471	1
196	1	17	LEU	H	H	8.813	1
197	1	17	LEU	N	N	124.259	1
198	1	18	MET	CA	C	57.353	1
199	1	18	MET	CB	C	35.184	1
200	1	18	MET	CE	C	17.364	1
201	1	18	MET	C	C	174.136	1
202	1	18	MET	HA	H	4.277	1
203	1	18	MET	HB3	H	1.415
204	1	18	MET	HB2	H	1.915
205	1	18	MET	HE1	H	1.676	1
206	1	18	MET	HE2	H	1.676	1
207	1	18	MET	HE3	H	1.676	1
208	1	18	MET	HG3	H	2.261
209	1	18	MET	HG2	H	2.672
210	1	18	MET	H	H	8.314	1
211	1	18	MET	N	N	126.334	1
212	1	19	GLN	CA	C	56.845	1
213	1	19	GLN	CB	C	30.531	1
214	1	19	GLN	CG	C	34.323	1
215	1	19	GLN	C	C	176.874	1
216	1	19	GLN	HA	H	4.322	1
217	1	19	GLN	HB2	H	2.105
218	1	19	GLN	HB3	H	2.105
219	1	19	GLN	HG2	H	2.33
220	1	19	GLN	HG3	H	2.33
221	1	19	GLN	H	H	9.598	1
222	1	19	GLN	N	N	122.037	1
223	1	20	ASP	CA	C	51.184	1
224	1	20	ASP	CB	C	40.958	1
225	1	20	ASP	HA	H	4.975	1
226	1	20	ASP	HB3	H	2.353
227	1	20	ASP	HB2	H	3.069
228	1	20	ASP	H	H	10.615	1
229	1	20	ASP	N	N	123.525	1
230	1	21	PRO	CA	C	62.019	1
231	1	21	PRO	CB	C	33.301	1
232	1	21	PRO	CD	C	49.86	1
233	1	21	PRO	C	C	178.05	1
234	1	21	PRO	HB2	H	2.263
235	1	21	PRO	HB3	H	2.263
236	1	21	PRO	HD2	H	3.744
237	1	21	PRO	HD3	H	3.744
238	1	21	PRO	HG2	H	1.929
239	1	21	PRO	HG3	H	1.929
240	1	22	VAL	CA	C	58.04	1
241	1	22	VAL	CB	C	35.455	1
242	1	22	VAL	CG1	C	20.99
243	1	22	VAL	CG2	C	20.05
244	1	22	VAL	C	C	172.48	1
245	1	22	VAL	HA	H	5.026	1
246	1	22	VAL	HB	H	1.728	1
247	1	22	VAL	HG11	H	0.683
248	1	22	VAL	HG12	H	0.683
249	1	22	VAL	HG13	H	0.683
250	1	22	VAL	HG21	H	0.914
251	1	22	VAL	HG22	H	0.914
252	1	22	VAL	HG23	H	0.914
253	1	22	VAL	H	H	9.918	1
254	1	22	VAL	N	N	120.022	1
255	1	23	ILE	CA	C	58.833	1
256	1	23	ILE	CB	C	42.728	1
257	1	23	ILE	CD1	C	13.39	1
258	1	23	ILE	CG1	C	14.84	1
259	1	23	ILE	CG2	C	15.197	1
260	1	23	ILE	C	C	176.565	1
261	1	23	ILE	HA	H	5.312	1
262	1	23	ILE	HB	H	1.618	1
263	1	23	ILE	HD11	H	0.909	1
264	1	23	ILE	HD12	H	0.909	1
265	1	23	ILE	HD13	H	0.909	1
266	1	23	ILE	HG12	H	1.264
267	1	23	ILE	HG13	H	1.264
268	1	23	ILE	HG21	H	0.734	1
269	1	23	ILE	HG22	H	0.734	1
270	1	23	ILE	HG23	H	0.734	1
271	1	23	ILE	H	H	9.699	1
272	1	23	ILE	N	N	123.034	1
273	1	24	VAL	CA	C	59.609	1
274	1	24	VAL	CB	C	33.915	1
275	1	24	VAL	C	C	178.665	1
276	1	24	VAL	HA	H	4.953	1
277	1	24	VAL	HB	H	1.319	1
278	1	24	VAL	HG11	H	0.951
279	1	24	VAL	HG12	H	0.951
280	1	24	VAL	HG13	H	0.951
281	1	24	VAL	HG21	H	0.951
282	1	24	VAL	HG22	H	0.951
283	1	24	VAL	HG23	H	0.951
284	1	24	VAL	H	H	7.847	1
285	1	24	VAL	N	N	117.586	1
286	1	25	SER	CA	C	62.521	1
287	1	25	SER	CB	C	63.041	1
288	1	25	SER	C	C	174.539	1
289	1	25	SER	HB2	H	4.096
290	1	25	SER	HB3	H	4.096
291	1	25	SER	H	H	9.383	1
292	1	25	SER	N	N	116.464	1
293	1	26	SER	CA	C	58.863	1
294	1	26	SER	CB	C	63.786	1
295	1	26	SER	C	C	176.124	1
296	1	26	SER	HA	H	4.46	1
297	1	26	SER	HB3	H	3.711
298	1	26	SER	HB2	H	4.269
299	1	26	SER	H	H	6.899	1
300	1	26	SER	N	N	114.512	1
301	1	27	GLY	CA	C	45.788	1
302	1	27	GLY	HA3	H	3.942
303	1	27	GLY	HA2	H	4.702
304	1	27	GLY	H	H	9.021	1
305	1	27	GLY	N	N	111.113	1
306	1	28	GLN	CA	C	54.087	1
307	1	28	GLN	CB	C	29.672	1
308	1	28	GLN	CG	C	34.318	1
309	1	28	GLN	C	C	173.404	1
310	1	28	GLN	HA	H	4.275	1
311	1	28	GLN	HB2	H	1.744
312	1	28	GLN	HB3	H	1.744
313	1	28	GLN	HG2	H	2.01
314	1	28	GLN	HG3	H	2.243
315	1	28	GLN	HE21	H	6.551
316	1	28	GLN	HE22	H	7.43
317	1	28	GLN	H	H	7.239	1
318	1	28	GLN	N	N	118.117	1
319	1	28	GLN	NE2	N	114.978	1
320	1	29	THR	CA	C	62.184	1
321	1	29	THR	CB	C	69.724	1
322	1	29	THR	CG2	C	23.008	1
323	1	29	THR	C	C	172.19	1
324	1	29	THR	HA	H	5.339	1
325	1	29	THR	HB	H	3.68	1
326	1	29	THR	HG21	H	1.095	1
327	1	29	THR	HG22	H	1.095	1
328	1	29	THR	HG23	H	1.095	1
329	1	29	THR	H	H	7.092	1
330	1	29	THR	N	N	118.257	1
331	1	30	TYR	CA	C	56.583	1
332	1	30	TYR	CB	C	45.236	1
333	1	30	TYR	C	C	175.414	1
334	1	30	TYR	HA	H	5.15	1
335	1	30	TYR	HB3	H	2.072
336	1	30	TYR	HB2	H	3.327
337	1	30	TYR	HD1	H	7.709
338	1	30	TYR	HD2	H	7.709
339	1	30	TYR	HE1	H	6.549
340	1	30	TYR	HE2	H	6.549
341	1	30	TYR	HH	H	9.47	1
342	1	30	TYR	H	H	9.178	1
343	1	30	TYR	N	N	121.756	1
344	1	31	GLU	CA	C	56.667	1
345	1	31	GLU	CB	C	29.505	1
346	1	31	GLU	CG	C	33.084	1
347	1	31	GLU	C	C	179.632	1
348	1	31	GLU	HA	H	4.58	1
349	1	31	GLU	HB2	H	2.17
350	1	31	GLU	HB3	H	2.17
351	1	31	GLU	HG2	H	2.384
352	1	31	GLU	HG3	H	2.384
353	1	31	GLU	H	H	10.358	1
354	1	31	GLU	N	N	125.436	1
355	1	32	ARG	CA	C	61.225	1
356	1	32	ARG	CB	C	29.67	1
357	1	32	ARG	C	C	177.654	1
358	1	32	ARG	HA	H	3.777	1
359	1	32	ARG	HB2	H	1.899
360	1	32	ARG	HB3	H	1.899
361	1	32	ARG	HD2	H	2.976
362	1	32	ARG	HD3	H	2.976
363	1	32	ARG	HG2	H	1.366
364	1	32	ARG	HG3	H	1.366
365	1	32	ARG	H	H	8.528	1
366	1	32	ARG	N	N	130.63	1
367	1	33	SER	CA	C	61.925	1
368	1	33	SER	C	C	177.053	1
369	1	33	SER	HA	H	4.152	1
370	1	33	SER	HB2	H	3.984
371	1	33	SER	HB3	H	3.984
372	1	33	SER	H	H	9.266	1
373	1	33	SER	N	N	111.611	1
374	1	34	CYS	CA	C	59.279	1
375	1	34	CYS	CB	C	28.09	1
376	1	34	CYS	C	C	178.112	1
377	1	34	CYS	HA	H	4.19	1
378	1	34	CYS	HB3	H	2.855
379	1	34	CYS	HB2	H	3.257
380	1	34	CYS	H	H	7.101	1
381	1	34	CYS	N	N	119.587	1
382	1	35	ILE	CA	C	59.182	1
383	1	35	ILE	CB	C	38.121	1
384	1	35	ILE	CD1	C	13.82	1
385	1	35	ILE	CG2	C	21.88	1
386	1	35	ILE	C	C	176.045	1
387	1	35	ILE	HA	H	4.167	1
388	1	35	ILE	HB	H	2.07	1
389	1	35	ILE	HD11	H	0.207	1
390	1	35	ILE	HD12	H	0.207	1
391	1	35	ILE	HD13	H	0.207	1
392	1	35	ILE	HG12	H	0.999
393	1	35	ILE	HG13	H	0.999
394	1	35	ILE	HG21	H	0.674	1
395	1	35	ILE	HG22	H	0.674	1
396	1	35	ILE	HG23	H	0.674	1
397	1	35	ILE	H	H	8.028	1
398	1	35	ILE	N	N	119.276	1
399	1	36	GLN	CA	C	58.947	1
400	1	36	GLN	CB	C	28.935	1
401	1	36	GLN	CG	C	33.66	1
402	1	36	GLN	C	C	177.724	1
403	1	36	GLN	HA	H	3.989	1
404	1	36	GLN	HB2	H	2.047
405	1	36	GLN	HB3	H	2.047
406	1	36	GLN	HE21	H	6.752
407	1	36	GLN	HE22	H	7.907
408	1	36	GLN	HG2	H	2.379
409	1	36	GLN	HG3	H	2.379
410	1	36	GLN	H	H	9.032	1
411	1	36	GLN	N	N	124.993	1
412	1	36	GLN	NE2	N	115.283	1
413	1	37	LYS	CA	C	59.85	1
414	1	37	LYS	CB	C	32.417	1
415	1	37	LYS	CD	C	28.7	1
416	1	37	LYS	CG	C	25.314	1
417	1	37	LYS	CE	C	42.21	1
418	1	37	LYS	C	C	179.135	1
419	1	37	LYS	HA	H	4.169	1
420	1	37	LYS	HB2	H	2.23
421	1	37	LYS	HB3	H	2.23
422	1	37	LYS	HD2	H	1.685
423	1	37	LYS	HD3	H	1.685
424	1	37	LYS	HE2	H	2.855
425	1	37	LYS	HE3	H	2.855
426	1	37	LYS	HG2	H	1.538
427	1	37	LYS	HG3	H	1.538
428	1	37	LYS	H	H	7.548	1
429	1	37	LYS	N	N	118.074	1
430	1	38	TRP	CA	C	60.2	1
431	1	38	TRP	CB	C	29.863	1
432	1	38	TRP	CD1	C	120.888	1
433	1	38	TRP	CE3	C	115.305	1
434	1	38	TRP	CH2	C	119.754	1
435	1	38	TRP	C	C	178.242	1
436	1	38	TRP	CZ2	C	109.05	1
437	1	38	TRP	CZ3	C	109.608	1
438	1	38	TRP	HA	H	4.45	1
439	1	38	TRP	HB3	H	3.221
440	1	38	TRP	HB2	H	3.663
441	1	38	TRP	HD1	H	6.78	1
442	1	38	TRP	HE1	H	10.081	1
443	1	38	TRP	HE3	H	7.459	1
444	1	38	TRP	HH2	H	6.795	1
445	1	38	TRP	H	H	7.441	1
446	1	38	TRP	HZ2	H	6.995	1
447	1	38	TRP	HZ3	H	7.455	1
448	1	38	TRP	N	N	121.987	1
449	1	38	TRP	NE1	N	130.052	1
450	1	39	LEU	CA	C	57.507	1
451	1	39	LEU	CB	C	41.22	1
452	1	39	LEU	CD1	C	22.53
453	1	39	LEU	CD2	C	22.53
454	1	39	LEU	C	C	182.169	1
455	1	39	LEU	HA	H	4.41	1
456	1	39	LEU	HB3	H	1.557
457	1	39	LEU	HB2	H	2.027
458	1	39	LEU	HD11	H	0.9
459	1	39	LEU	HD12	H	0.9
460	1	39	LEU	HD13	H	0.9
461	1	39	LEU	HD21	H	0.9
462	1	39	LEU	HD22	H	0.9
463	1	39	LEU	HD23	H	0.9
464	1	39	LEU	H	H	8.439	1
465	1	39	LEU	N	N	119.81	1
466	1	40	ASP	CA	C	57.044	1
467	1	40	ASP	CB	C	40.09	1
468	1	40	ASP	C	C	177.924	1
469	1	40	ASP	HA	H	4.474	1
470	1	40	ASP	HB2	H	2.779
471	1	40	ASP	HB3	H	2.779
472	1	40	ASP	H	H	9.268	1
473	1	40	ASP	N	N	122.195	1
474	1	41	SER	CA	C	59.035	1
475	1	41	SER	CB	C	63.346	1
476	1	41	SER	C	C	173.781	1
477	1	41	SER	HA	H	4.422	1
478	1	41	SER	HB3	H	3.965
479	1	41	SER	HB2	H	3.965
480	1	41	SER	H	H	7.634	1
481	1	41	SER	N	N	113.604	1
482	1	42	GLY	CA	C	44.867	1
483	1	42	GLY	C	C	173.703	1
484	1	42	GLY	HA3	H	3.535
485	1	42	GLY	HA2	H	4.005
486	1	42	GLY	H	H	7.775	1
487	1	42	GLY	N	N	107.485	1
488	1	43	HIS	CA	C	56.302	1
489	1	43	HIS	CB	C	29.801	1
490	1	43	HIS	CD2	C	115.93	1
491	1	43	HIS	HA	H	4.176	1
492	1	43	HIS	HB3	H	1.258
493	1	43	HIS	HB2	H	2.255
494	1	43	HIS	HD2	H	5.99	1
495	1	43	HIS	H	H	6.679	1
496	1	43	HIS	N	N	117.756	1
497	1	44	LYS	CA	C	54.685	1
498	1	44	LYS	CB	C	32.741	1
499	1	44	LYS	CG	C	25.18	1
500	1	44	LYS	CE	C	42.21	1
501	1	44	LYS	C	C	175.824	1
502	1	44	LYS	HA	H	4.508	1
503	1	44	LYS	HB3	H	1.581
504	1	44	LYS	HB2	H	2.043
505	1	44	LYS	HG2	H	1.277
506	1	44	LYS	HG3	H	1.277
507	1	44	LYS	HD2	H	1.585
508	1	44	LYS	HD3	H	1.585
509	1	44	LYS	HE2	H	2.917
510	1	44	LYS	HE3	H	2.917
511	1	45	THR	CA	C	58.624	1
512	1	45	THR	CB	C	71.99	1
513	1	45	THR	CG2	C	22.3	1
514	1	45	THR	C	C	172.549	1
515	1	45	THR	HA	H	4.789	1
516	1	45	THR	HB	H	3.727	1
517	1	45	THR	HG21	H	0.972	1
518	1	45	THR	HG22	H	0.972	1
519	1	45	THR	HG23	H	0.972	1
520	1	45	THR	H	H	7.129	1
521	1	45	THR	N	N	107.806	1
522	1	46	CYS	CA	C	57.082	1
523	1	46	CYS	CB	C	29.195	1
524	1	46	CYS	HA	H	4.336	1
525	1	46	CYS	HB2	H	2.854
526	1	46	CYS	HB3	H	2.854
527	1	46	CYS	H	H	8.565	1
528	1	46	CYS	N	N	124.545	1
529	1	47	PRO	CA	C	53.788	1
530	1	47	PRO	CB	C	30.81	1
531	1	47	PRO	CD	C	49.92	1
532	1	47	PRO	CG	C	25.72	1
533	1	47	PRO	C	C	177.453	1
534	1	47	PRO	HA	H	4.357	1
535	1	47	PRO	HB3	H	0.842
536	1	47	PRO	HB2	H	1.208
537	1	47	PRO	HG2	H	-0.157
538	1	47	PRO	HG3	H	-0.157
539	1	47	PRO	HD3	H	3.277
540	1	47	PRO	HD2	H	3.648
541	1	48	LYS	CA	C	58.468	1
542	1	48	LYS	CB	C	34.427	1
543	1	48	LYS	CE	C	42.21	1
544	1	48	LYS	CG	C	25.14	1
545	1	48	LYS	CD	C	29.053	1
546	1	48	LYS	C	C	177.864	1
547	1	48	LYS	HA	H	4.149	1
548	1	48	LYS	HB2	H	1.643
549	1	48	LYS	HB3	H	1.643
550	1	48	LYS	HG2	H	1.279
551	1	48	LYS	HG3	H	1.279
552	1	48	LYS	HE2	H	2.906
553	1	48	LYS	HE3	H	2.906
554	1	48	LYS	H	H	8.094	1
555	1	48	LYS	N	N	116.332	1
556	1	49	THR	CA	C	62.63	1
557	1	49	THR	CB	C	71.973	1
558	1	49	THR	CG2	C	21.33	1
559	1	49	THR	C	C	175.714	1
560	1	49	THR	HA	H	4.393	1
561	1	49	THR	HB	H	4.398	1
562	1	49	THR	HG21	H	1.207	1
563	1	49	THR	HG22	H	1.207	1
564	1	49	THR	HG23	H	1.207	1
565	1	49	THR	H	H	8.284	1
566	1	49	THR	N	N	109.008	1
567	1	50	GLN	CA	C	57.864	1
568	1	50	GLN	CB	C	26.185	1
569	1	50	GLN	CG	C	33.64	1
570	1	50	GLN	C	C	174.424	1
571	1	50	GLN	HA	H	3.906	1
572	1	50	GLN	HB2	H	2.304
573	1	50	GLN	HB3	H	2.304
574	1	50	GLN	HG2	H	2.352
575	1	50	GLN	HG3	H	2.352
576	1	50	GLN	H	H	8.609	1
577	1	50	GLN	N	N	116.322	1
578	1	51	GLN	CA	C	53.788	1
579	1	51	GLN	CB	C	29.785	1
580	1	51	GLN	CG	C	33.828	1
581	1	51	GLN	HA	H	4.582	1
582	1	51	GLN	HB2	H	1.748
583	1	51	GLN	HB3	H	1.748
584	1	51	GLN	HE21	H	6.256
585	1	51	GLN	HE22	H	6.546
586	1	51	GLN	HG3	H	2.009
587	1	51	GLN	HG2	H	2.362
588	1	51	GLN	H	H	7.331	1
589	1	51	GLN	N	N	117.915	1
590	1	52	PRO	CA	C	63.208	1
591	1	52	PRO	CB	C	31.84	1
592	1	52	PRO	CD	C	50.82	1
593	1	52	PRO	C	C	177.165	1
594	1	52	PRO	HA	H	4.359	1
595	1	52	PRO	HB3	H	1.755
596	1	52	PRO	HB2	H	2.334
597	1	52	PRO	HD3	H	3.671
598	1	52	PRO	HD2	H	3.916
599	1	52	PRO	HG2	H	2.088
600	1	52	PRO	HG3	H	2.088
601	1	53	LEU	CD2	C	22.78
602	1	53	LEU	CD1	C	22.78
603	1	53	LEU	HA	H	4.35	1
604	1	53	LEU	HB2	H	1.377
605	1	53	LEU	HB3	H	1.377
606	1	53	LEU	HD11	H	0.764
607	1	53	LEU	HD12	H	0.764
608	1	53	LEU	HD13	H	0.764
609	1	53	LEU	HD21	H	0.956
610	1	53	LEU	HD22	H	0.956
611	1	53	LEU	HD23	H	0.956
612	1	53	LEU	HG	H	1.711	1
613	1	53	LEU	H	H	8.268	1
614	1	53	LEU	N	N	123.908	1
615	1	54	SER	CA	C	59.874	1
616	1	54	SER	CB	C	62.917	1
617	1	54	SER	C	C	174.075	1
618	1	54	SER	HA	H	4.252	1
619	1	54	SER	HB2	H	3.925
620	1	54	SER	HB3	H	3.925
621	1	55	HIS	CA	C	55.409	1
622	1	55	HIS	CB	C	32.335	1
623	1	55	HIS	HA	H	4.746	1
624	1	55	HIS	HB2	H	3.23
625	1	55	HIS	HB3	H	3.23
626	1	55	HIS	HD2	H	6.886	1
627	1	55	HIS	H	H	7.551	1
628	1	55	HIS	N	N	115.879	1
629	1	56	THR	HB	H	4.073	1
630	1	56	THR	HG21	H	1.348	1
631	1	56	THR	HG22	H	1.348	1
632	1	56	THR	HG23	H	1.348	1
633	1	56	THR	H	H	7.869	1
634	1	56	THR	N	N	120.9	1
635	1	58	LEU	HA	H	4.215	1
636	1	59	THR	CG2	C	21	1
637	1	59	THR	HB	H	3.904	1
638	1	59	THR	HG21	H	1.257	1
639	1	59	THR	HG22	H	1.257	1
640	1	59	THR	HG23	H	1.257	1
641	1	60	PRO	HA	H	4.214	1
642	1	60	PRO	HB2	H	1.945
643	1	60	PRO	HB3	H	1.945
644	1	61	ASN	HA	H	4.746	1
645	1	61	ASN	H	H	8.181	1
646	1	61	ASN	N	N	119.794	1
647	1	62	PHE	CE1	C	126.962
648	1	62	PHE	CE2	C	126.962
649	1	62	PHE	CA	C	62.34	1
650	1	62	PHE	CB	C	38.406	1
651	1	62	PHE	CD1	C	126.962
652	1	62	PHE	CD2	C	126.962
653	1	62	PHE	CZ	C	133.973	1
654	1	62	PHE	HA	H	4.229	1
655	1	62	PHE	HB3	H	2.892
656	1	62	PHE	HB2	H	3.155
657	1	62	PHE	HD1	H	7.289
658	1	62	PHE	HD2	H	7.289
659	1	62	PHE	HE1	H	7.521
660	1	62	PHE	HE2	H	7.521
661	1	62	PHE	H	H	7.378	1
662	1	62	PHE	HZ	H	7.554	1
663	1	62	PHE	N	N	119.544	1
664	1	63	VAL	CG1	C	21.51
665	1	63	VAL	CG2	C	21.51
666	1	63	VAL	HA	H	4.158	1
667	1	63	VAL	HB	H	2.17	1
668	1	63	VAL	HG11	H	1.055
669	1	63	VAL	HG12	H	1.055
670	1	63	VAL	HG13	H	1.055
671	1	63	VAL	HG21	H	1.055
672	1	63	VAL	HG22	H	1.055
673	1	63	VAL	HG23	H	1.055
674	1	64	LEU	CA	C	56.98	1
675	1	64	LEU	CB	C	39.492	1
676	1	64	LEU	CD1	C	22.162
677	1	64	LEU	CD2	C	22.162
678	1	64	LEU	C	C	177.946	1
679	1	64	LEU	HA	H	4.063	1
680	1	64	LEU	HB2	H	1.594
681	1	64	LEU	HB3	H	1.594
682	1	64	LEU	HD11	H	1.07
683	1	64	LEU	HD12	H	1.07
684	1	64	LEU	HD13	H	1.07
685	1	64	LEU	HD21	H	1.07
686	1	64	LEU	HD22	H	1.07
687	1	64	LEU	HD23	H	1.07
688	1	64	LEU	H	H	6.724	1
689	1	64	LEU	N	N	122.04	1
690	1	65	LYS	CA	C	59.695	1
691	1	65	LYS	CB	C	32.236	1
692	1	65	LYS	CE	C	42.59	1
693	1	65	LYS	CG	C	25	1
694	1	65	LYS	C	C	178.325	1
695	1	65	LYS	HA	H	3.915	1
696	1	65	LYS	HB2	H	1.637
697	1	65	LYS	HB3	H	1.637
698	1	65	LYS	HD2	H	2.173
699	1	65	LYS	HD3	H	2.173
700	1	65	LYS	HE2	H	3.092
701	1	65	LYS	HE3	H	3.092
702	1	65	LYS	HG2	H	1.12
703	1	65	LYS	HG3	H	1.12
704	1	65	LYS	H	H	8.019	1
705	1	65	LYS	N	N	118.598	1
706	1	66	SER	CB	C	62.998	1
707	1	66	SER	C	C	177.156	1
708	1	66	SER	HA	H	4.231	1
709	1	66	SER	HB2	H	4.031
710	1	66	SER	HB3	H	4.031
711	1	66	SER	H	H	8.015	1
712	1	66	SER	N	N	113.887	1
713	1	67	LEU	CA	C	58.329	1
714	1	67	LEU	CB	C	40.622	1
715	1	67	LEU	CD1	C	22.484
716	1	67	LEU	CD2	C	26.431
717	1	67	LEU	C	C	179.943	1
718	1	67	LEU	HA	H	4.071	1
719	1	67	LEU	HB3	H	1.555
720	1	67	LEU	HB2	H	1.944
721	1	67	LEU	HD11	H	0.764
722	1	67	LEU	HD12	H	0.764
723	1	67	LEU	HD13	H	0.764
724	1	67	LEU	HD21	H	0.897
725	1	67	LEU	HD22	H	0.897
726	1	67	LEU	HD23	H	0.897
727	1	67	LEU	HG	H	1.791	1
728	1	67	LEU	H	H	7.813	1
729	1	67	LEU	N	N	121.692	1
730	1	68	ILE	CA	C	66.091	1
731	1	68	ILE	CB	C	37.996	1
732	1	68	ILE	CD1	C	13.92	1
733	1	68	ILE	CG1	C	28.875	1
734	1	68	ILE	CG2	C	17.27	1
735	1	68	ILE	C	C	178.576	1
736	1	68	ILE	HA	H	2.921	1
737	1	68	ILE	HB	H	1.636	1
738	1	68	ILE	HD11	H	0.651	1
739	1	68	ILE	HD12	H	0.651	1
740	1	68	ILE	HD13	H	0.651	1
741	1	68	ILE	HG12	H	-0.581
742	1	68	ILE	HG13	H	-0.581
743	1	68	ILE	HG21	H	0.883	1
744	1	68	ILE	HG22	H	0.883	1
745	1	68	ILE	HG23	H	0.883	1
746	1	68	ILE	H	H	8.603	1
747	1	68	ILE	N	N	122.005	1
748	1	69	SER	CA	C	62.647	1
749	1	69	SER	C	C	177.326	1
750	1	69	SER	HA	H	4.127	1
751	1	69	SER	HB2	H	4.096
752	1	69	SER	HB3	H	4.096
753	1	69	SER	H	H	8.527	1
754	1	69	SER	N	N	117.006	1
755	1	70	GLN	CA	C	59.164	1
756	1	70	GLN	CB	C	29.360	1
757	1	70	GLN	CG	C	34.75	1
758	1	70	GLN	C	C	178.501	1
759	1	70	GLN	HA	H	4.116	1
760	1	70	GLN	HB2	H	2.157
761	1	70	GLN	HB3	H	2.157
762	1	70	GLN	HG2	H	2.6
763	1	70	GLN	HG3	H	2.6
764	1	70	GLN	H	H	8.107	1
765	1	70	GLN	N	N	120.462	1
766	1	71	TRP	CA	C	62.425	1
767	1	71	TRP	CB	C	29.626	1
768	1	71	TRP	CD1	C	121.735	1
769	1	71	TRP	CE3	C	115.625	1
770	1	71	TRP	CH2	C	119.482	1
771	1	71	TRP	C	C	179.795	1
772	1	71	TRP	CZ2	C	109.576	1
773	1	71	TRP	CZ3	C	115.586	1
774	1	71	TRP	HA	H	4.167	1
775	1	71	TRP	HB2	H	3.635
776	1	71	TRP	HB3	H	3.635
777	1	71	TRP	HD1	H	7.436	1
778	1	71	TRP	HE1	H	10.279	1
779	1	71	TRP	HH2	H	7.094	1
780	1	71	TRP	H	H	8.308	1
781	1	71	TRP	N	N	123.493	1
782	1	71	TRP	NE1	N	130.178	1
783	1	72	CYS	CA	C	65.059	1
784	1	72	CYS	CB	C	26.356	1
785	1	72	CYS	C	C	177.157	1
786	1	72	CYS	HA	H	3.825	1
787	1	72	CYS	HB2	H	3.21
788	1	72	CYS	HB3	H	3.21
789	1	72	CYS	H	H	9.237	1
790	1	72	CYS	N	N	119.412	1
791	1	73	GLU	CA	C	59.398	1
792	1	73	GLU	CB	C	29.53	1
793	1	73	GLU	CG	C	36.19	1
794	1	73	GLU	C	C	179.462	1
795	1	73	GLU	HA	H	4.073	1
796	1	73	GLU	HB2	H	2.087
797	1	73	GLU	HB3	H	2.087
798	1	73	GLU	HG2	H	2.336
799	1	73	GLU	HG3	H	2.336
800	1	73	GLU	H	H	8.073	1
801	1	73	GLU	N	N	119.799	1
802	1	74	ALA	CA	C	54.283	1
803	1	74	ALA	CB	C	18.568	1
804	1	74	ALA	C	C	178.554	1
805	1	74	ALA	HA	H	4.065	1
806	1	74	ALA	HB1	H	1.358	1
807	1	74	ALA	HB2	H	1.358	1
808	1	74	ALA	HB3	H	1.358	1
809	1	74	ALA	H	H	7.868	1
810	1	74	ALA	N	N	120.49	1
811	1	75	ASN	CA	C	53.588	1
812	1	75	ASN	CB	C	39.821	1
813	1	75	ASN	C	C	174.094	1
814	1	75	ASN	HA	H	4.524	1
815	1	75	ASN	HB3	H	1.365
816	1	75	ASN	HB2	H	2.314
817	1	75	ASN	HD21	H	6.125
818	1	75	ASN	HD22	H	6.374
819	1	75	ASN	H	H	7.280	1
820	1	75	ASN	N	N	112.955	1
821	1	76	GLY	CA	C	47.306	1
822	1	76	GLY	C	C	174.193	1
823	1	76	GLY	HA2	H	3.841
824	1	76	GLY	HA3	H	3.841
825	1	76	GLY	H	H	7.504	1
826	1	76	GLY	N	N	108.812	1
827	1	77	ILE	CA	C	59.857	1
828	1	77	ILE	CB	C	39.017	1
829	1	77	ILE	CG1	C	27.47	1
830	1	77	ILE	CG2	C	17.194	1
831	1	77	ILE	C	C	175.188	1
832	1	77	ILE	HA	H	4.085	1
833	1	77	ILE	HB	H	1.608	1
834	1	77	ILE	HD11	H	0.91	1
835	1	77	ILE	HD12	H	0.91	1
836	1	77	ILE	HD13	H	0.91	1
837	1	77	ILE	HG12	H	1.345
838	1	77	ILE	HG13	H	1.345
839	1	77	ILE	HG21	H	0.929	1
840	1	77	ILE	HG22	H	0.929	1
841	1	77	ILE	HG23	H	0.929	1
842	1	77	ILE	H	H	8.043	1
843	1	77	ILE	N	N	120.932	1
844	1	78	GLU	CA	C	55.968	1
845	1	78	GLU	CB	C	30.735	1
846	1	78	GLU	CG	C	36.3	1
847	1	78	GLU	C	C	174.256	1
848	1	78	GLU	HA	H	4.238	1
849	1	78	GLU	HB2	H	1.874
850	1	78	GLU	HB3	H	1.874
851	1	78	GLU	HG2	H	2.177
852	1	78	GLU	HG3	H	2.177
853	1	78	GLU	H	H	8.421	1
854	1	78	GLU	N	N	127.275	1
855	1	79	LEU	CA	C	52.475	1
856	1	79	LEU	CB	C	44.325	1
857	1	79	LEU	CD1	C	23.29
858	1	79	LEU	CD2	C	23.29
859	1	79	LEU	HA	H	4.622	1
860	1	79	LEU	HB2	H	1.683
861	1	79	LEU	HB3	H	1.683
862	1	79	LEU	HD11	H	0.927
863	1	79	LEU	HD12	H	0.927
864	1	79	LEU	HD13	H	0.927
865	1	79	LEU	HD21	H	0.927
866	1	79	LEU	HD22	H	0.927
867	1	79	LEU	HD23	H	0.927
868	1	79	LEU	H	H	8.361	1
869	1	79	LEU	N	N	126.672	1
870	1	80	PRO	CD	C	50.34	1
871	1	80	PRO	CG	C	27.545	1
872	1	80	PRO	HA	H	4.315	1
873	1	80	PRO	HB2	H	1.639
874	1	80	PRO	HB3	H	1.639
875	1	80	PRO	HD3	H	3.625
876	1	80	PRO	HD2	H	3.801
877	1	80	PRO	HG2	H	1.967
878	1	80	PRO	HG3	H	1.967

Release date

2023-08-01

Citation

Release of a Ubiquitin Brake Activates Pattern Recognition Receptor-mediated Immunity in Rice
Zhang, Y., Yu, C.Z.

Related entities 1. U-box domain-containing protein 12 (E.C.2.3.2.27), : 1 : 4 : 225 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Agilent DD2 - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.5 mM [U-100% 13C; U-100% 15N] SD-Ubox, 10 mM TRIS, 100 mM sodium chloride, 1 mM PMSF, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

3 2D 1H-13C HSQC aromatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

4 3D 1H-13C NOESY aliphatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

5 3D 1H-13C NOESY aromatic

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

6 3D CBCA(CO)NH

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

7 3D HNCACB

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

8 3D HNCA

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

9 3D 1H-15N TOCSY

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

10 3D HCCH-TOCSY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

11 3D 1H-15N NOESY

Instrument

Agilent DD2 - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

12 3D HNCO

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	U-box domain-containing protein 12	[U-13C; U-15N]	protein	0.0 ~ 0.0 mM
2	DTT	natural abundance	reducing agent	2 mM
3	sodium chloride	natural abundance	salt	100 mM
4	TRIS	natural abundance	buffer	10 mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36527_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36527_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	starch_output	Warning	878		97.5 (chain: 1, length: 80)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: starch_output
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 80, Sequence coverage: 97.5%
- Total number of assignments
  - ¹³C chemical shifts: 286
  - ¹H chemical shifts: 523
  - ¹⁵N chemical shifts: 69
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
30	TYR	HH	9.47

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	365	286	78.4
¹H chemical shifts	477	426	89.3
¹⁵N chemical shifts	83	69	83.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	160	129	80.6
¹H chemical shifts	157	141	89.8
¹⁵N chemical shifts	73	65	89.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	205	157	76.6
¹H chemical shifts	320	285	89.1
¹⁵N chemical shifts	10	4	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	45	38	84.4
¹H chemical shifts	45	41	91.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	28	21	75.0
¹H chemical shifts	30	25	83.3
¹⁵N chemical shifts	2	2	100.0

Keywords PLANT PROTEIN, S237D, Ubox, ligase, ubiquitination

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Found 1 Document (0.601 seconds)

BMRB: 36527

Related entities 1. U-box domain-containing protein 12 (E.C.2.3.2.27), : 1 : 4 : 225 entities Detail

Experiments performed 12 experiments Detail

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Chemical shift validation 4 contents Detail