BMRB: 4090

Solution NMR Structure of the I gamma Subdomain of the Mu end DNA Binding Domain of Mu Phage Transposase, Minimized Average Structure

Authors

Clore, G.M., Clubb, R.T., Schumaker, S., Gronenborn, A.M.

Assembly

Transposase

Entity

1. Transposase (polymer, Thiol state: not reported), 75 monomers, 8866.004 Da Detail

MNVHKSEFDE DAWQFLIADY LRPEKPAFRK CYERLELAAR EHGWSIPSRA TAFRRIQQLD EAMVVACREG EHALM

Formula weight

8866.004 Da

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 77.0 %, Completeness (bb): 82.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.0 % (693 of 900)	79.9 % (377 of 472)	69.1 % (242 of 350)	94.9 % (74 of 78)
Backbone	82.0 % (364 of 444)	96.6 % (144 of 149)	67.7 % (151 of 223)	95.8 % (69 of 72)
Sidechain	75.8 % (401 of 529)	72.1 % (233 of 323)	81.5 % (163 of 200)	83.3 % (5 of 6)
Aromatic	46.7 % (43 of 92)	47.8 % (22 of 46)	45.5 % (20 of 44)	50.0 % (1 of 2)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. Transposase

MNVHKSEFDE DAWQFLIADY LRPEKPAFRK CYERLELAAR EHGWSIPSRA TAFRRIQQLD EAMVVACREG EHALM

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Sample

Temperature 303 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	Transposase			0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: -0.53 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 na	null	null	0.0
1H	na	na	0.0 na	null	null	0.0
15N	na	na	0.0 na	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.53 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 na	null	null	0.0
1H	na	na	0.0 na	null	null	0.0
15N	na	na	0.0 na	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 na	null	null	0.0
1H	na	na	0.0 na	null	null	0.0
15N	na	na	0.0 na	null	null	0.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 2EZH, Strand ID: A Detail

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Release date

2000-04-03

Citation

Solution structure of the I gamma subdomain of the Mu end DNA-binding domain of phage Mu transposase
Clubb, R.T., Schumacher, S., Mizuuchi, K., Gronenborn, A.M., Clore, G.M.
J. Mol. Biol. (1997), 273, 19-25, PubMed 9367742 , DOI 10.1006/jmbi.1997.1312 , Abstract

Related entities 1. Transposase, : 1 : 1 : 2 : 6 entities Detail

Experiments performed 1 experiments Detail