BMRB: 4120

NMR Structure of a Classical Pseudoknot: Interplay of Single- and Double-Stranded RNA

Authors

Kolk, M.H., Van der Graaf, M., Wijmenga, S.S., Pleij, C.W.A., Heus, H.A., Hilbers, C.W.

Assembly

TYMV pseudoknot

Entity

1. TYMV pseudoknot (polymer, Thiol state: not present), 44 monomers, 14047.30 Da Detail

GGGAGCUCAA CUCUCCCCCC CUUUUCCGAG GGUCAUCGGA ACCA

Formula weight

14047.3 Da

Source organism

Turnip yellow mosaic virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.8 %, Completeness (bb): 99.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C
All	91.8 % (636 of 693)	85.8 % (339 of 395)	99.7 % (297 of 298)
Suger, PO4	99.2 % (480 of 484)	98.9 % (261 of 264)	99.5 % (219 of 220)
Nucleobase	74.6 % (156 of 209)	59.5 % (78 of 131)	100.0 % (78 of 78)
Aromatic	89.1 % (156 of 175)	80.4 % (78 of 97)	100.0 % (78 of 78)

1. TYMV pseudoknot

GGGAGCUCAA CUCUCCCCCC CUUUUCCGAG GGUCAUCGGA ACCA

Show sample condition

Sample

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	TYMV pseudoknot			1.0 ~ 2.5 mM
2	TYMV pseudoknot	[13C/15N adenine]		1.0 ~ 2.5 mM
3	TYMV pseudoknot	[13C/15N uridine]		1.0 ~ 2.5 mM
4	MgCl2			10 mM

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Release date

2001-06-18

Citation

NMR structure of a classical pseudoknot: interplay of single- and double-stranded RNA
Kolk, M.H., Van der Graaf, M., Wijmenga, S.S., Pleij, C.W.A., Heus, H.A., Hilbers, C.W.
Science (1998), 280, 434-438, PubMed 9545221 , DOI: , Abstract

Related entities 1. TYMV pseudoknot, : 1 : 1 entities Detail

Experiments performed 7 experiments Detail