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BMRB: 4129


4129
1Q80
1H and 15N Resonance Assignment of the Calcium-bound Form of the Nereis Diversicolor Sarcoplasmic Calcium(2+)-binding Protein.
Authors
Craescu, C.T., Precheur, B., van Riel, A., Sakamoto, H., Engelborghs, Y., Cox, J.A.
Assembly
Nereis diversicolor sarcoplasmic calcium-binding protein
Entity
1. calcium-binding protein (polymer), 174 monomers, 19527.78 Da Detail

XDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV


2. calcium-binding protein (polymer), 174 monomers, 19485.74 Da Detail

SDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV


3. CA (non-polymer), 40.078 Da
Total weight
39053.598 Da
Max. entity weight
19527.78 Da
Source organism
Hediste diversicolor
Exptl. method
NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 49.4 %, Completeness: 42.5 %, Completeness (bb): 49.0 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All42.5 % (1036 of 2439)41.4 % (858 of 2072)48.5 % (178 of 367)
Backbone49.0 % (513 of 1047)48.9 % (347 of 710)49.3 % (166 of 337)
Sidechain37.6 % (523 of 1392)37.5 % (511 of 1362)40.0 % (12 of 30)
Aromatic48.6 % (103 of 212)48.5 % (100 of 206)50.0 % (3 of 6)
Methyl49.4 % (84 of 170)49.4 % (84 of 170)

1. calcium-binding protein

XDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

2. calcium-binding protein

SDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

Show sample condition
Sample

Temperature 308 K, pH 6.5, Details recombinant sample


#NameIsotope labelingTypeConcentration
1calcium-binding protein[U-15N]1.0 mM
2H2O93 %
3D2O7 %

Chem. Shift Complete2
Sequence coverage: 8.6 %, Completeness: 24.3 %, Completeness (bb): 28.1 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All24.3 % (1187 of 4878)23.8 % (985 of 4144)27.5 % (202 of 734)
Backbone28.1 % (588 of 2094)28.1 % (399 of 1420)28.0 % (189 of 674)
Sidechain21.5 % (598 of 2784)21.5 % (585 of 2724)21.7 % (13 of 60)
Aromatic26.9 % (114 of 424)26.9 % (111 of 412)25.0 % (3 of 12)
Methyl27.9 % (95 of 340)27.9 % (95 of 340)

1. calcium-binding protein

XDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

2. calcium-binding protein

SDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

Show sample condition
Sample

Temperature 308 K, pH 6.5, Details natural protein sample where it is acetylated


#NameIsotope labelingTypeConcentration
4calcium-binding protein1.2 mM
5H2O93 %
6D2O7 %

Chem. Shift Complete3
Sequence coverage: 10.9 %, Completeness: 18.1 %, Completeness (bb): 21.4 % Detail
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Polymer type: polypeptide(L)

Total1H15N
All18.1 % (1322 of 7317)17.6 % (1093 of 6216)20.8 % (229 of 1101)
Backbone21.4 % (672 of 3141)21.5 % (457 of 2130)21.3 % (215 of 1011)
Sidechain15.6 % (650 of 4176)15.6 % (636 of 4086)15.6 % (14 of 90)
Aromatic18.2 % (116 of 636)18.3 % (113 of 618)16.7 % (3 of 18)
Methyl20.4 % (104 of 510)20.4 % (104 of 510)

1. calcium-binding protein

XDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

2. calcium-binding protein

SDLWVQKMKT YFNRIDFDKD GAITRMDFES MAERFAKESE MKAEHAKVLM DSLTGVWDNF LTAVAGGKGI DETTFINSMK EMVKNPEAKS VVEGPLPLFF RAVDTNEDNN ISRDEYGIFF GMLGLDKTMA PASFDAIDTN NDGLLSLEEF VIAGSDFFMN DGDSTNKVFW GPLV

Show sample condition
Sample

Temperature 308 K, pH 6.5, Details natural protein sample where it is acetylated


#NameIsotope labelingTypeConcentration
4calcium-binding protein1.2 mM
5H2O93 %
6D2O7 %

Protein Blocks Logo
Calculated from 17 models in PDB: 1Q80, Strand ID: A Detail

Release date
2000-04-02
Citation
1H and 15N resonance assignment of the calcium-bound form of the Nereis diversicolor sarcoplasmic Ca(2+)-binding protein
Craescu, C.T., Precheur, B., van Riel, A., Sakamoto, H., Engelborghs, Y., Cox, J.A.
J. Biomol. NMR (1998), 12, 565-566, PubMed 9862132 ,
Related entities 1. calcium-binding protein, : 1 : 86 entities Detail
More details for 87 related entities
Related entities 2. calcium-binding protein, : 1 : 85 entities Detail
More details for 86 related entities
Experiments performed 1 experiments Detail
nullKeywords calcium-bound form, NMR, nuclear magnetic resonance, NSCP, Nereis sarcoplasmic calcium-binding protein, protein, resonance assignment