BMRBj - BMREntryMaster

Found 1 Document (1.554 seconds)

BMRB: 4148

4BMF

Solution Structure of the Cellulose-binding Domain of Endoglucanase I from Trichoderma reesei and Its Interaction with Cello-oligosaccharides

Authors

Mattinen, M., Linder, M., Drakenberg, T., Annila, A.

Assembly

cellulose-binding domain

Entity

1. cellulose-binding domain (polymer, Thiol state: all disulfide bound), 38 monomers, 4093.429 Da Detail

SCTQTHWGQC GGIGYSGCKT CTSGTTCQYS NDYYSQCL

Formula weight

4093.429 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS18:SG
2	disulfide	sing	1:CYS10:SG	1:CYS27:SG
3	disulfide	sing	1:CYS21:SG	1:CYS37:SG

Source organism

Trichoderma reesei

Exptl. method

NMR

Refine. method

DISTANCE-GEOMETRY DG REFINED BY SIMULATED ANNEALING SA, AMBER FORCE FIELD IN MOLECULAR DYNAMICS COMPUTATION MD AND CONJUGATE GRADIENTS ALGORITHM IN FINAL MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.0 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	97.0 % (194 of 200)	97.0 % (194 of 200)
Backbone	92.7 % (76 of 82)	92.7 % (76 of 82)
Sidechain	100.0 % (118 of 118)	100.0 % (118 of 118)
Aromatic	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. cellulose-binding domain

SCTQTHWGQC GGIGYSGCKT CTSGTTCQYS NDYYSQCL

Show sample condition

Sample

Temperature 293 K, pH 3.90 (±0.005)

#	Name	Isotope labeling	Type	Concentration
1	cellulose-binding domain			27 mg/mL

Protein Blocks Logo

Calculated from 19 models in PDB: 4BMF, Strand ID: A Detail

Release date

2000-03-01

Citation

Solution structure of the cellulose-binding domain of endoglucanase I from Trichoderma reesei and its interaction with cello-oligosaccharides
Mattinen, M., Linder, M., Drakenberg, T., Annila, A.
Eur. J. Biochem. (1998), 256, 279-286, PubMed 9760165 , DOI: , Abstract

Related entities 1. cellulose-binding domain, : 1 : 1 : 109 entities Detail

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Found 1 Document (1.554 seconds)

BMRB: 4148

Sample

Related entities 1. cellulose-binding domain, : 1 : 1 : 109 entities Detail