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BMRB: 4172


4172
1BN9
Response Element of the Orphan Nuclear Receptor Rev-erb Beta
Authors
Castagne, C., Terenzi, H., Zakin, M.M., Delepierre, M.
Assembly
Rev-erb beta response element
Entity
1. Rev-RE subunit 1 (polymer, Thiol state: not present), 15 monomers, 4736.030 Da Detail

TAGAATGTAG GTCAG


2. Rev-RE subunit 2 (polymer, Thiol state: not present), 15 monomers, 4566.920 Da Detail

CTGACCTACA TTCTA


Total weight
9302.949 Da
Max. entity weight
4736.03 Da
Source organism
Mus musculus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 50.0 %, Completeness: 42.1 %, Completeness (bb): 42.1 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H31P
All42.1 % (135 of 321)41.6 % (121 of 291)46.7 % (14 of 30)
Suger, PO442.1 % (101 of 240)41.4 % (87 of 210)46.7 % (14 of 30)
Nucleobase42.0 % (34 of 81)42.0 % (34 of 81)
Aromatic46.7 % (28 of 60)46.7 % (28 of 60)
Methyl44.4 % (4 of 9)44.4 % (4 of 9)

1. Rev-RE subunit 1

TAGAATGTAG GTCAG

2. Rev-RE subunit 2

CTGACCTACA TTCTA

Show sample condition
Sample

Temperature 303 (±0.2) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Rev-RE subunit 13.6 mM
2Rev-RE subunit 23.6 mM

Chem. Shift Complete2
Sequence coverage: 50.0 %, Completeness: 43.3 %, Completeness (bb): 41.7 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H31P
All43.3 % (278 of 642)43.0 % (250 of 582)46.7 % (28 of 60)
Suger, PO441.7 % (200 of 480)41.0 % (172 of 420)46.7 % (28 of 60)
Nucleobase48.1 % (78 of 162)48.1 % (78 of 162)
Aromatic47.5 % (57 of 120)47.5 % (57 of 120)
Methyl50.0 % (9 of 18)50.0 % (9 of 18)

1. Rev-RE subunit 1

TAGAATGTAG GTCAG

2. Rev-RE subunit 2

CTGACCTACA TTCTA

Show sample condition
Sample

Temperature 303 (±0.2) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Rev-RE subunit 13.6 mM
2Rev-RE subunit 23.6 mM

Release date
2000-12-13
Citation
Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*
Castagne, C., Terenzi, H., Zakin, M.M., Delepierre, M.
Biochimie (2000), 82, 739-748, PubMed 11018291 , DOI 10.1016/s0300-9084(00)01148-2 ,
Experiments performed 2 experiments Detail
nullKeywords DNA, NMR, nuclear magnetic resonance, orphan nuclear receptor, response element