BMRB: 4187

Nuclear Magnetic Resonance Structure of d(GCATATGATAG).d(CTATCATATGC): A Consensus Sequence for Promoters Recognized by Sigma-K RNA Polymerase

Authors

Tonelli, M., Ragg, E., Bianucci, A.M., Lesiak, K., James, T.L.

Assembly

Sigma-K RNA Polymerase Promoter Consensus Sequence

Entity

1. Promoter strand 1 (polymer, Thiol state: not present), 11 monomers, 3460.219 Da Detail

GCATATGATA G

2. Promoter strand 2 (polymer, Thiol state: not present), 11 monomers, 3371.157 Da Detail

CTATCATATG C

Total weight

6831.376 Da

Max. entity weight

3460.219 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 50.0 %, Completeness: 41.3 %, Completeness (bb): 39.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	41.3 % (88 of 213)	41.3 % (88 of 213)
Suger, PO4	39.6 % (61 of 154)	39.6 % (61 of 154)
Nucleobase	45.8 % (27 of 59)	45.8 % (27 of 59)
Aromatic	50.0 % (22 of 44)	50.0 % (22 of 44)
Methyl	42.9 % (3 of 7)	42.9 % (3 of 7)

1. Promoter strand 1

GCATATGATA G

2. Promoter strand 2

CTATCATATG C

Show sample condition

Sample

Pressure 1 atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Promoter strand 1			2.5 mM
2	Promoter strand 2			2.5 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 42.7 %, Completeness (bb): 39.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	42.7 % (182 of 426)	42.7 % (182 of 426)
Suger, PO4	39.9 % (123 of 308)	39.9 % (123 of 308)
Nucleobase	50.0 % (59 of 118)	50.0 % (59 of 118)
Aromatic	50.0 % (44 of 88)	50.0 % (44 of 88)
Methyl	50.0 % (7 of 14)	50.0 % (7 of 14)

1. Promoter strand 1

GCATATGATA G

2. Promoter strand 2

CTATCATATG C

Show sample condition

Sample

Pressure 1 atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Promoter strand 1			2.5 mM
2	Promoter strand 2			2.5 mM

Hide sample codition

Coupling constant

48 J values in 2 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.2) Detail

---

Pressure 1 atm, Temperature 298.0 (±0.1) K, pH 7.0 (±0.2)

List #1 coupling_constants_one, Field strength (¹H) 600 MHz

List #2 coupling_constants_two, Field strength (¹H) 600 MHz

Release date

2000-01-10

Citation

Nuclear magnetic resonance structure of d(GCATATGATAG). d(CTATCATATGC): a consensus sequence for promoters recognized by sigma K RNA polymerase
Tonelli, M., Ragg, E., Bianucci, A.M., Lesiak, K., James, T.L.
Biochemistry (1998), 37, 11745-11761, PubMed 9718297 , DOI 10.1021/bi980481n , Abstract

Experiments performed 2 experiments Detail