BMRB: 4189

Solution Structure of Reduced Horse Heart Cytochrome c

Authors

Banci, L., Bertini, I., Huber, J.G., Spyroulias, G.A., Turano, P.

Assembly

cytochrome c

Entity

1. cytochrome c (polymer), 104 monomers, 11701.40 Da Detail

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

2. HEC (non-polymer), 618.503 Da

Total weight

12319.903 Da

Max. entity weight

11701.4 Da

Entity Connection

thioether 2, metal coordination 2 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	thioether	sing	2:HEC1:CAB	1:CYS14:SG
2	thioether	sing	2:HEC1:CAC	1:CYS17:SG
3	metal coordination	sing	2:HEC1:FE	1:HIS18:NE2
4	metal coordination	sing	2:HEC1:FE	1:MET80:SD

Source organism

Equus caballus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING, TORSION ANGLE DYNAMICS, RESTRAINED ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 88.6 %, Completeness (bb): 98.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	88.6 % (591 of 667)	88.6 % (591 of 667)
Backbone	98.6 % (213 of 216)	98.6 % (213 of 216)
Sidechain	83.8 % (378 of 451)	83.8 % (378 of 451)
Aromatic	100.0 % (48 of 48)	100.0 % (48 of 48)
Methyl	95.7 % (44 of 46)	95.7 % (44 of 46)

1. cytochrome c

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

Show sample condition

Sample

Pressure 1013 mbar, Temperature 293 (±1) K, pH 7.0 (±0.1), Details The sample was lyophilized in a phosphate solution. Reduction of the protein was accomplished by addition of sodium dithionite.

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c			2.5 ~ 3.0 mM
2	phosphate			100 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 15.4 %, Completeness: 47.7 %, Completeness (bb): 54.9 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	47.7 % (636 of 1334)	47.7 % (636 of 1334)
Backbone	54.9 % (237 of 432)	54.9 % (237 of 432)
Sidechain	44.2 % (399 of 902)	44.2 % (399 of 902)
Aromatic	50.0 % (48 of 96)	50.0 % (48 of 96)
Methyl	52.2 % (48 of 92)	52.2 % (48 of 92)

1. cytochrome c

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

Show sample condition

Sample

Pressure 1013 mbar, Temperature 293 (±1) K, pH 7.0 (±0.1), Details The sample was lyophilized in a phosphate solution. Reduction of the protein was accomplished by addition of sodium dithionite.

#	Name	Isotope labeling	Type	Concentration
1	cytochrome c			2.5 ~ 3.0 mM
2	phosphate			100 mM

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 1GIW, Strand ID: A Detail

---

Release date

2000-03-07

Citation 1

Solution structure of reduced horse heart cytochrome c
Banci, L., Bertini, I., Huber, J.G., Spyroulias, G.A., Turano, P.
J. Biol. Inorg. Chem. (1999), 4, 21-31, PubMed 10499099 , DOI: , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. cytochrome c, : 1 : 30 : 2 : 1 : 206 entities Detail

Interaction partners 1. cytochrome c, : 1 interactors Detail

Experiments performed 2 experiments Detail