BMRB: 4195

Solution NMR Structure of the Complex of Alpha-Bungarotoxin with a Library-Derived Peptide (NMR, Average Structure)

Authors

Scherf, T., Balass, M., Fuchs, S., Katchalski-Katzir, E., Anglister, J.

Assembly

Alpha-Bungaratoxin/Library Peptide Complex

Entity

1. alpha-Bungarotoxin (polymer), 74 monomers, 7994.189 Da Detail

IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG

2. synthetic peptide (polymer), 13 monomers, 1638.797 Da Detail

MRYYESSLKS YPD

Total weight

9632.986 Da

Max. entity weight

7994.189 Da

Source organism

Bungarus multicinctus

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY/ DYNAMICAL SIMMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.1 %, Completeness: 80.3 %, Completeness (bb): 82.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.3 % (407 of 507)	80.3 % (407 of 507)
Backbone	82.8 % (140 of 169)	82.8 % (140 of 169)
Sidechain	76.3 % (258 of 338)	76.3 % (258 of 338)
Aromatic	65.7 % (23 of 35)	65.7 % (23 of 35)
Methyl	93.8 % (30 of 32)	93.8 % (30 of 32)

1. alpha-Bungarotoxin

IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG

2. synthetic peptide

MRYYESSLKS YPD

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	alpha-Bungarotoxin			5 mM
2	synthetic peptide			5 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 2BTX, Strand ID: A, B Detail

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Release date

2000-03-21

Citation 1

Three-dimensional solution structure of the complex of alpha-bungarotoxin with a library-derived peptide
Scherf, T., Balass, M., Fuchs, S., Katchalski-Katzir, E., Anglister, J.
Proc. Natl. Acad. Sci. U. S. A. (1997), 94, 6059-6064, PubMed 9177168 , DOI: , Abstract

Show more 1 citaion

Citation 2

Primary structure of alpha-bungarotoxin. Six amino acid residues differ from the previously reported sequence
Ohta, M., Ohta, K., Nishitani, H., Hayashi, K.
FEBS Lett. (1987), 222, 79-82, PubMed 3653402 , DOI: , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 2. synthetic peptide, : 1 : 1 : 4 entities Detail

Experiments performed 4 experiments Detail