BMRBj - BMREntryMaster

Found 1 Document (3.188 seconds)

BMRB: 4198

1BR0

Three-dimensional Structure of an Evolutionarily Conserved N-terminal Domain of Syntaxin 1A

Authors

Fernandez, I., Ubach, J., Dulubova, I., Zhang, X., Sudhof, T.C., Rizo, J.

Assembly

N-terminal domain of syntaxin 1A

Entity

1. N-terminal domain of syntaxin 1A (polymer), 161 monomers, 18269.98 Da Detail

GSPGISGGGG GIPASMKDRT QELRTAKDSD DDDDVTVTVD RDRFMDEFFE QVEEIRGFID KIAENVEEVK RKHSAILASP NPDEKTKEEL EELMSDIKKT ANKVRSKLKS IEQSIEQEEG LNRSSADLRI RKTQHSTLSR KFVEVMSEYN ATQSDYRERS K

Formula weight

18269.98 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 79.5 %, Completeness: 79.1 %, Completeness (bb): 78.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.1 % (1465 of 1853)	79.6 % (780 of 980)	78.2 % (551 of 705)	79.8 % (134 of 168)
Backbone	78.7 % (754 of 958)	77.7 % (254 of 327)	79.3 % (376 of 474)	79.0 % (124 of 157)
Sidechain	79.7 % (834 of 1047)	80.6 % (526 of 653)	77.8 % (298 of 383)	90.9 % (10 of 11)
Aromatic	79.7 % (59 of 74)	91.9 % (34 of 37)	67.6 % (25 of 37)
Methyl	78.9 % (112 of 142)	77.5 % (55 of 71)	80.3 % (57 of 71)

1. N-terminal domain of Syntaxin 1A

GSPGISGGGG GIPASMKDRT QELRTAKDSD DDDDVTVTVD RDRFMDEFFE QVEEIRGFID KIAENVEEVK RKHSAILASP NPDEKTKEEL EELMSDIKKT ANKVRSKLKS IEQSIEQEEG LNRSSADLRI RKTQHSTLSR KFVEVMSEYN ATQSDYRERS K

Show sample condition

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	-379.6 ppm	external	direct	0.0

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	-379.6 ppm	external	direct	0.0

Referencing offset: -0.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	-379.6 ppm	external	direct	0.0

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	-379.6 ppm	external	direct	0.0

Referencing offset: -0.69 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1BR0, Strand ID: A Detail

Release date

1999-11-30

Citation

Three-dimensional structure of an evolutionarily conserved N-terminal domain of syntaxin 1A
Fernandez, I., Ubach, J., Dulubova, I., Zhang, X., Sudhof, T.C., Rizo, J.
Cell (1998), 94, 841-849, PubMed 9753330 , DOI: , Abstract

Related entities 1. N-terminal domain of syntaxin 1A, : 9 : 49 entities Detail

Interaction partners 1. N-terminal domain of syntaxin 1A, : 24 interactors Detail

More details for 24 interactors identified by 13 citations

Experiments performed 10 experiments Detail

1 1N-15N TOCSY-HMQC

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

2 1H-15N NOESY-HMQC

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

3 HNCO

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

4 HNCACB

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

5 (H)CBCACO(CA)HA

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

6 (H)C(CO)NH-TOCSY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

7 H(C)(CO)NH-TOCSY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

8 HCCH-TOCSY

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

9 3D 1H-15N NOESY-HSQC

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

10 4D 1H-13C,1H-13C HMQC-NOESY-HMQC

Instrument

Model Varian UNITY (500 MHz)

Sample #1

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of Syntaxin 1A	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 305 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-terminal domain of Syntaxin 1A	[U-15N]		1.0 mM

null

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Found 1 Document (3.188 seconds)

BMRB: 4198

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Chemical shift reference

Related entities 1. N-terminal domain of syntaxin 1A, : 9 : 49 entities Detail

Interaction partners 1. N-terminal domain of syntaxin 1A, : 24 interactors Detail

Experiments performed 10 experiments Detail

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