BMRB: 4199

Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3

Authors

Miura, K., Ohgiya, S., Hoshino, T., Nemoto, N., Odaira, M., Nitta, K., Tsuda, S.

Assembly

N-term linker Type III Antifreeze protein RD3

Entity

1. N-term linker Type III Antifreeze protein RD3 (polymer), 73 monomers, 7749.088 Da Detail

NKASVVANQL IPINTALTLI MMKAEVVTPM GIPAEEIPNL VGMQVNRAVP LGTTLMPDMV KNYEDGTTSP GLK

Formula weight

7749.088 Da

Source organism

Lycodichthys dearborni

Exptl. method

NMR

Refine. method

SA

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.0 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	96.0 % (479 of 499)	95.5 % (406 of 425)	98.6 % (73 of 74)
Backbone	98.1 % (206 of 210)	97.9 % (141 of 144)	98.5 % (65 of 66)
Sidechain	94.5 % (273 of 289)	94.3 % (265 of 281)	100.0 % (8 of 8)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	96.2 % (51 of 53)	96.2 % (51 of 53)

1. N-term. domain with linker Type III Antifreeze protein RD3

NKASVVANQL IPINTALTLI MMKAEVVTPM GIPAEEIPNL VGMQVNRAVP LGTTLMPDMV KNYEDGTTSP GLK

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Sample

Temperature 277 (±0.1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	N-term. domain with linker Type III Antifreeze protein RD3	[U-15N]		1.0 mM

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Protein Blocks Logo

Calculated from 40 models in PDB: 3NLA, Strand ID: A Detail

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Coupling constant

50 J values in 1 lists
Temperature 277 (±0.1) K, pH 6.7 (±0.2) Detail

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Temperature 277 (±0.1) K, pH 6.7 (±0.2)

List #1 coupling_constants_1

Release date

1999-12-21

Citation 1

Determination of the solution structure of the N-domain plus linker of Antarctic eel pout antifreeze protein RD3
Miura, K., Ohgiya, S., Hoshino, T., Nemoto, N., Odaira, M., Nitta, K., Tsuda, S.
J. Biochem. (1999), 126, 387-394, PubMed 10423534 , DOI: , Abstract

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Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , DOI: , Abstract

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Related entities 1. N-term linker Type III Antifreeze protein RD3, : 1 : 4 : 2 : 73 entities Detail

Experiments performed 6 experiments Detail