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BMRB: 4206

1MFN

Solution NMR Structure of Linked Cell Attachment Modules of Mouse Fibronectin Containing the RGD and Synergy Regions, 20 Structures

Authors

Copie, V., Tomita, Y., Akiyama, S.K., Aota, S., Yamada, K.M., Venable, R.M., Pastor, R.W., Krueger, S., Torchia, D.A.

Assembly

fibronectin

Entity

1. fibronectin (polymer), 185 monomers, 19892.95 Da Detail

MGLDSPTGFD SSDITANSFT VHWVAPRAPI TGYIIRHHAE HSVGRPRQDR VPPSRNSITL TNLNPGTEYV VSIIAVNGRE ESPPLIGQQA TVSDIPRDLE VIASTPTSLL ISWEPPAVSV RYYRITYGET GGNSPVQEFT VPGSKSTATI NNIKPGADYT ITLYAVTGRG DSPASSKPVS INYKT

Formula weight

19892.95 Da

Source organism

Exptl. method

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 85.8 %, Completeness (bb): 83.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.8 % (1757 of 2047)	93.2 % (982 of 1054)	74.0 % (600 of 811)	96.2 % (175 of 182)
Backbone	83.1 % (893 of 1074)	97.8 % (358 of 366)	68.8 % (372 of 541)	97.6 % (163 of 167)
Sidechain	90.1 % (1031 of 1144)	90.7 % (624 of 688)	89.6 % (395 of 441)	80.0 % (12 of 15)
Aromatic	59.0 % (79 of 134)	62.7 % (42 of 67)	55.4 % (36 of 65)	50.0 % (1 of 2)
Methyl	100.0 % (218 of 218)	100.0 % (109 of 109)	100.0 % (109 of 109)

1. fibronectin

MGLDSPTGFD SSDITANSFT VHWVAPRAPI TGYIIRHHAE HSVGRPRQDR VPPSRNSITL TNLNPGTEYV VSIIAVNGRE ESPPLIGQQA TVSDIPRDLE VIASTPTSLL ISWEPPAVSV RYYRITYGET GGNSPVQEFT VPGSKSTATI NNIKPGADYT ITLYAVTGRG DSPASSKPVS INYKT

Show sample condition

Sample

Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1MFN, Strand ID: A Detail

Release date

2000-03-09

Citation

Solution structure and dynamics of linked cell attachment modules of mouse fibronectin containing the RGD and synergy regions: comparison with the human fibronectin crystal structure
Copie, V., Tomita, Y., Akiyama, S.K., Aota, S., Yamada, K.M., Venable, R.M., Pastor, R.W., Krueger, S., Torchia, D.A.
J. Mol. Biol. (1998), 277, 663-682, PubMed 9533887 , DOI: , Abstract

Related entities 1. fibronectin, : 1 : 3 : 774 entities Detail

Interaction partners 1. fibronectin, : 14 interactors Detail

More details for 14 interactors identified by 5 citations

Experiments performed 18 experiments Detail

1 CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

2 CBCANH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

3 HBHA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

4 C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

5 H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

6 HCCH-TOCSY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

7 HOHAHA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

8 15N,13C-HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

9 2D-NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

10 3D-NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

11 4D-NOESY

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

12 HNHA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

13 HNHB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

14 HAHB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

15 CCO-SED

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

16 CN-SED

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

17 LRCC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

18 LRCH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Temperature 308 K, pH 4.2

#	Name	Isotope labeling	Type	Concentration
1	fibronectin			0.0 ~ 0.0 mM
2	NaCl			50 mM

nullKeywords cell adhesion protein, extracellular matrix, RGD