BMRB: 4208

The Y64A Mutant of Cytochrome c553 from Desulfovibrio vulgaris Hildenborough

Authors

DOLLA, A., MARION, D., SEBBAN-KREUZER, C., BLACKLEDGE, M.J., GUERLESQUIN, F.

Assembly

CytC(Y64A)

Entity

1. CytC(Y64A) (polymer, Thiol state: all other bound), 79 monomers, 8211.322 Da Detail

ADGAALYKSC IGCHGADGSK AAMGSAKPVK GQGAEELYKK MKGYADGSYG GERKAMMTNA VKKASDEELK ALADYMSKL

2. HEM (non-polymer), 616.487 Da

Total weight

8827.81 Da

Max. entity weight

8211.322 Da

Source organism

Desulfovibrio desulfuricans

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING AND RESTRAINED MOLECULAR DYNAMICS ENERGY REFINEMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 92.1 %, Completeness (bb): 96.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.1 % (409 of 444)	92.1 % (409 of 444)
Backbone	96.4 % (162 of 168)	96.4 % (162 of 168)
Sidechain	89.5 % (247 of 276)	89.5 % (247 of 276)
Aromatic	100.0 % (22 of 22)	100.0 % (22 of 22)
Methyl	96.8 % (30 of 31)	96.8 % (30 of 31)

1. CytC(Y64A)

ADGAALYKSC IGCHGADGSK AAMGSAKPVK GQGAEELYKK MKGYADGSYG GERKAMMTNA VKKASDEELK ALADYMSKL

Show sample condition

Sample

Temperature 310 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	CytC(Y64A)			0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 39 models in PDB: 2DVH, Strand ID: A Detail

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Release date

2007-07-12

Citation

Structure and dynamics of ferrocytochrome c553 from Desulfovibrio vulgaris studied by NMR spectroscopy and restrained molecular dynamics
Blackledge, M.J., Medvedeva, S., Poncin, M., Guerlesquin, F., Bruschi, M., Marion, D.
J. Mol. Biol. (1995), 245, 661-681, PubMed 7844834 , DOI 10.1006/jmbi.1994.0054 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. CytC(Y64A), : 1 : 4 : 48 entities Detail

Experiments performed 2 experiments Detail