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BMRB: 4214

2TMP

N-terminal Domain of Tissue Inhibitor of Metalloproteinase-2 (N-TIMP-2)

Authors

Muskett, F.W., Frenkiel, T.A., Feeney, J., Freedman, R.B., Carr, M.D., Williamson, R.A.

Assembly

Tissue Inhibitor of Metalloproteinases-2 (TIMP-2)

Entity

1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) (polymer, Thiol state: all disulfide bound), 127 monomers, 14090.00 Da Detail

CSCSPVHPQQ AFCNADVVIR TKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCE

Formula weight

14090.0 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS72:SG
2	disulfide	sing	1:CYS3:SG	1:CYS102:SG
3	disulfide	sing	1:CYS13:SG	1:CYS126:SG

Source organism

Exptl. method

Refine. method

SIMULATED ANNEALING USING TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.0 %, Completeness (bb): 79.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.0 % (1206 of 1471)	91.4 % (702 of 768)	66.7 % (381 of 571)	93.2 % (123 of 132)
Backbone	79.9 % (599 of 750)	95.3 % (246 of 258)	64.7 % (240 of 371)	93.4 % (113 of 121)
Sidechain	85.6 % (717 of 838)	89.4 % (456 of 510)	79.2 % (251 of 317)	90.9 % (10 of 11)
Aromatic	29.8 % (31 of 104)	46.2 % (24 of 52)	11.8 % (6 of 51)	100.0 % (1 of 1)
Methyl	97.6 % (123 of 126)	96.8 % (61 of 63)	98.4 % (62 of 63)

1. tissue inhibitor of metalloproteinases-2 (TIMP-2)

CSCSPVHPQQ AFCNADVVIR TKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCE

Show sample condition

Sample

Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

Protein Blocks Logo

Calculated from 49 models in PDB: 2TMP, Strand ID: A Detail

Coupling constant

164 J values in 1 lists
Pressure 1 atm, Temperature 308 (±0.1) K Detail

Release date

2000-06-16

Citation 1

High resolution structure of the N-terminal domain of tissue inhibitor of metalloproteinases-2 and characterization of its interaction site with matrix metalloproteinase-3
Muskett, F.W., Frenkiel, T.A., Feeney, J., Freedman, R.B., Carr, M.D., Williamson, R.A.
J. Biol. Chem. (1998), 273, 21736-21743, PubMed 9705310 , DOI: , Abstract

Show more 2 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 3

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Hide non-primary 2 citaions

Related entities 1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2), : 1 : 6 : 63 entities Detail

Interaction partners 1. Tissue Inhibitor of Metalloproteinases-2 (TIMP-2), : 24 interactors Detail

More details for 24 interactors identified by 4 citations

Experiments performed 12 experiments Detail

1 2D NOESY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

2 2D TOCSY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

3 2D COSY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

4 3D NOESY-TOCSY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

5 2D HSQC

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

6 3D 15N NOESY HSQC

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

7 3D 15N HNHA

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

8 3D 15N HNHB

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

9 3D HCCH TOCSY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

10 3D CBCA(CO)NH

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

11 3D CBCAHN

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

12 3D 13C HMQC NOESY

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 308 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	tissue inhibitor of metalloproteinases-2 (TIMP-2)	[U-96% 13C; U-98% 15N]		1.4 mM
2	Sodium Phosphate			25 mM
3	potassium Chloride			100 mM

nullKeywords Matrix Metalloproteinase Inhibitor, MMP Inhibitor, OB-fold, TIMP