BMRB: 4225

NMR structure of Escherichia coli glutaredoxin3-glutathione mixed disulfide complex

Authors

Nordstrand, K., Aslund, F., Holmgren, A., Otting, G., Berndt, K.D.

Assembly

Glutaredoxin 3 system

Entity

1. Glutaredoxin 3 system (polymer), 83 monomers, 9339.474 Da Detail

ANVEIYTKET CPYSHRAKAL LSSKGVSFQE LPIDGNAAKR EEMIKRSGRT TVPQIFIDAQ HIGGYDDLYA LDARGGLDPL LKX

Formula weight

9339.474 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:GSH83:SG2

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 99.0 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	99.0 % (567 of 573)	99.0 % (485 of 490)	98.8 % (82 of 83)
Backbone	98.0 % (240 of 245)	98.2 % (164 of 167)	97.4 % (76 of 78)
Sidechain	97.9 % (321 of 328)	97.8 % (316 of 323)	100.0 % (5 of 5)
Aromatic	100.0 % (30 of 30)	100.0 % (30 of 30)
Methyl	95.7 % (44 of 46)	95.7 % (44 of 46)

1. Glutaredoxin 3

ANVEIYTKET CPYSHRAKAL LSSKGVSFQE LPIDGNAAKR EEMIKRSGRT TVPQIFIDAQ HIGGYDDLYA LDARGGLDPL LKX

Show sample condition

Sample #1

Pressure 1 atm, Temperature 301 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Glutaredoxin 3			2.8 mM

Sample #2

Pressure 1 atm, Temperature 301 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Glutaredoxin 3	[U-15N]		5.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 3GRX, Strand ID: A Detail

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Release date

1998-08-16

Citation 1

NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: implications for the enzymatic mechanism
Nordstrand, K., Aslund, F., Holmgren, A., Otting, G., Berndt, K.D.
J. Mol. Biol. (1999), 286, 541-552, PubMed 9973569 , DOI 10.2210/pdb3grx/pdb , Abstract

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Citation 2

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Related entities 1. Glutaredoxin 3 system, : 1 : 3 : 257 entities Detail

Experiments performed 1 experiments Detail