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BMRB: 4226

1LDZ

The Lead-Dependent Ribozyme

Authors

Hoogstraten, C.G., Legault, P., Pardi, A.

Assembly

lead-dependent ribozyme

Entity

1. lead-dependent ribozyme (polymer), 30 monomers, 9824.875 Da Detail

GCGACCGAGC CAGCGAAAGU UGGGAGUCGC

Formula weight

9824.875 Da

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.7 %, Completeness (bb): 100.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	98.7 % (466 of 472)	98.8 % (256 of 259)	100.0 % (198 of 198)	80.0 % (12 of 15)
Suger, PO4	100.0 % (330 of 330)	100.0 % (180 of 180)	100.0 % (150 of 150)
Nucleobase	95.8 % (136 of 142)	96.2 % (76 of 79)	100.0 % (48 of 48)	80.0 % (12 of 15)
Aromatic	95.2 % (120 of 126)	95.2 % (60 of 63)	100.0 % (48 of 48)	80.0 % (12 of 15)

1. lead-dependent ribozyme

GCGACCGAGC CAGCGAAAGU UGGGAGUCGC

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

Release date

1999-12-22

Citation

Order, dynamics and metal-binding in the lead-dependent ribozyme
Legault, P., Hoogstraten, C.G., Metlitzky, E., Pardi, A.
J. Mol. Biol. (1998), 284, 325-335, PubMed 9813121 , DOI 10.1006/jmbi.1998.2181 , Abstract

Related entities 1. lead-dependent ribozyme, : 1 : 1 entities Detail

Experiments performed 10 experiments Detail

1 NOESY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

2 CPMG-NOESY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

3 BD-NOESY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

4 CBD-NOESY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

5 NOESY-HSQC

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

6 CBD-NOESY-HSQC

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

7 HMQC-NOESY-HSQC

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

8 HCCH-E.COSY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

9 directed HCC-TOCSY-CCH-E.COSY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

10 spin-echo difference CT-HCCH-COSY

Instrument

Varian UnityPlus - 500 MHz

Sample

State isotropic, Pressure 1 atm, Temperature 298 (±0.5) K, Details Sample annealed by 2 min @ 65 C, 5 min on ice prior to each use.

#	Name	Isotope labeling	Type	Concentration
1	lead-dependent ribozyme	[U-99% 13C; U-99% 15N]		1.2 mM
2	sodium phosphate			10 mM
3	sodium chloride			100 mM
4	EDTA			0.2 mM

nullKeywords Catalytic RNA, internal loops, leadzyme, NMR spectroscopy, RNA structure