BMRB: 4227

NMR structures (20) of the J-domain (residues 1-77) of the Escherichia coli N-terminal fragment (residues 1-78) of the Molecular Chaperone DNAJ

Authors

Huang, K., Flanagan, J.M., Prestegard, J.H.

Assembly

DNAJ

Entity

1. DNAJ (polymer, Thiol state: not present), 78 monomers, 9093.015 Da Detail

AKQDYYEILG VSKTAEEREI RKAYKRLAMK YHPDRNQGDK EAEAKFKEIK EAYEVLTDSQ KRAAYDQYGH AAFEQGGM

Formula weight

9093.015 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

DG/SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.3 %, Completeness (bb): 97.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.3 % (889 of 933)	94.3 % (466 of 494)	95.8 % (341 of 356)	98.8 % (82 of 83)
Backbone	97.6 % (455 of 466)	97.5 % (156 of 160)	97.4 % (223 of 229)	98.7 % (76 of 77)
Sidechain	93.7 % (506 of 540)	92.8 % (310 of 334)	95.0 % (190 of 200)	100.0 % (6 of 6)
Aromatic	92.9 % (78 of 84)	92.9 % (39 of 42)	92.9 % (39 of 42)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. DNAJ

AKQDYYEILG VSKTAEEREI RKAYKRLAMK YHPDRNQGDK EAEAKFKEIK EAYEVLTDSQ KRAAYDQYGH AAFEQGGM

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Sample

Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	DNAJ			0.0 ~ 0.0 mM
2	phosphate			50 mM

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 1BQZ, Strand ID: A Detail

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Release date

2001-07-08

Citation

The influence of C-terminal extension on the structure of the J-domain in E. Coli DNAJ
Huang, K., Flanagan, J., Prestegard, J.
Protein Sci. (1999), 8, 203-214, DOI:

Related entities 1. DNAJ, : 1 : 7 : 7 : 1 : 270 entities Detail

Interaction partners 1. DNAJ, : 96 interactors Detail