BMRB: 4228

NMR structures (20) of the J-Domain (residues 1-77) of the Escherichia coli n-terminal fragment (residues 1-104) of the molecular chaperone DNAJ

Authors

Huang, K., Flanagan, J.M., Prestegard, J.H.

Assembly

DNAJ

Entity

1. DNAJ (polymer, Thiol state: not present), 103 monomers, 11400.41 Da Detail

AKQDYYEILG VSKTAEEREI RKAYKRLAMK YHPDRNQGDK EAEAKFKEIK EAYEVLTDSQ KRAAYDQYGH AAFEQGGMGG GGFGGGADFS DIFGDVFGDI FGG

Formula weight

11400.41 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

DG/SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.2 %, Completeness: 83.3 %, Completeness (bb): 83.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.3 % (979 of 1175)	84.0 % (516 of 614)	82.3 % (373 of 453)	83.3 % (90 of 108)
Backbone	83.4 % (514 of 616)	81.4 % (180 of 221)	85.3 % (250 of 293)	82.4 % (84 of 102)
Sidechain	84.7 % (547 of 646)	85.5 % (336 of 393)	83.0 % (205 of 247)	100.0 % (6 of 6)
Aromatic	52.2 % (70 of 134)	52.2 % (35 of 67)	52.2 % (35 of 67)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. DNAJ

AKQDYYEILG VSKTAEEREI RKAYKRLAMK YHPDRNQGDK EAEAKFKEIK EAYEVLTDSQ KRAAYDQYGH AAFEQGGMGG GGFGGGADFS DIFGDVFGDI FGG

Show sample condition

Sample

Temperature 303 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	DNAJ			0.0 ~ 0.0 mM
2	phosphate			50 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1BQ0, Strand ID: A Detail

---

Release date

2001-07-08

Citation

The influence of c-terminal extension on the structure of the j-domain in e. coli dnaj"
Huang, K., Flanagan, J., Prestegard, J.
Protein Sci. (1998), 5587-5596, PubMed , DOI:

Related entities 1. DNAJ, : 1 : 6 : 3 : 1 : 178 entities Detail

Interaction partners 1. DNAJ, : 96 interactors Detail