BMRB: 4244

NMR Solution Structure of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2]

Authors

Aramini, J.M., Mujeeb, A., Germann, M.W.

Assembly

alphaT decamer duplex

Entity

1. alphaT decamer duplex (polymer, Thiol state: not present), 10 monomers, 3107.976 × 2 Da Detail

GCGAATXCGC

Total weight

6215.952 Da

Max. entity weight

3107.976 Da

Entity Connection

covalent 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:DT6:O3'	1:ATD7:P
2	covalent	sing	1:ATD7:O5'	1:DT6:P
3	covalent	sing	2:DT6:O3'	1:ATD7:P
4	covalent	sing	2:ATD7:O5'	1:DT6:P

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.0 %, Completeness: 90.7 %, Completeness (bb): 95.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	90.7 % (88 of 97)	90.9 % (80 of 88)	88.9 % (8 of 9)
Suger, PO4	95.8 % (69 of 72)	96.8 % (61 of 63)	88.9 % (8 of 9)
Nucleobase	76.0 % (19 of 25)	76.0 % (19 of 25)
Aromatic	100.0 % (18 of 18)	100.0 % (18 of 18)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. alphaT decamer DNA

GCGAATXCGC

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	alphaT decamer DNA			6.8 mM
2	NaCl			50 mM
3	Na phosphate			10 mM
4	EDTA			0.1 mM
5	D2O			100 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, Details Imino proton chemical shifts were obtained in the identical buffer as above, except the solvent was 90:10 H2O/D2O

#	Name	Isotope labeling	Type	Concentration
6	alphaT decamer DNA			6.8 mM
7	NaCl			50 mM
8	Na phosphate			10 mM
9	EDTA			0.1 mM
10	D2O			10 %
11	H2O			90 %

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Release date

1998-10-13

Citation

NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control
Aramini, J.M., Mujeeb, A., Germann, M.W.
Nucleic Acids Res. (1998), 26, 5644-5654, PubMed 9837995 , DOI: , Abstract

Experiments performed 2 experiments Detail