BMRB: 4255

Human Translation Initiation Factor eIF1

Authors

Fletcher, C.M., Pestova, T.V., Hellen, C.U.T., Wagner, G.

Assembly

Eukaryotic translation initiation factor 1

Entity

1. Eukaryotic translation initiation factor 1 (polymer), 126 monomers, 14299.98 Da Detail

MRGSHHHHHH TDPMSAIQNL HSFDPFADAS KGDDLLPAGT EDYIHIRIQQ RNGRKTLTTV QGIADDYDKK KLVKAFKKKF ACNGTVIEHP EYGEVIQLQG DQRKNICQFL VEIGLAKDDQ LKVHGF

Formula weight

14299.98 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 82.5 %, Completeness (bb): 95.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.5 % (1226 of 1486)	81.7 % (635 of 777)	80.5 % (462 of 574)	95.6 % (129 of 135)
Backbone	95.1 % (711 of 748)	95.0 % (245 of 258)	95.1 % (350 of 368)	95.1 % (116 of 122)
Sidechain	73.3 % (626 of 854)	75.1 % (390 of 519)	69.3 % (223 of 322)	100.0 % (13 of 13)
Aromatic	38.7 % (48 of 124)	69.4 % (43 of 62)	8.1 % (5 of 62)
Methyl	88.7 % (110 of 124)	93.5 % (58 of 62)	83.9 % (52 of 62)

1. eIF

MRGSHHHHHH TDPMSAIQNL HSFDPFADAS KGDDLLPAGT EDYIHIRIQQ RNGRKTLTTV QGIADDYDKK KLVKAFKKKF ACNGTVIEHP EYGEVIQLQG DQRKNICQFL VEIGLAKDDQ LKVHGF

Show sample condition

Sample #1

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	eIF	[U-100% 15N]		1.0 ~ 2.0 mM
2	Sodium Phosphate			50 mM
3	dtt			1 mM
4	EDTA			1 mM
5	Azide			0.1 mM
6	H2O			90 %
7	D2O			10 %

Sample #2

Temperature 300 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
8	eIF	[U-100% 15N; U-100% 13C]		1.0 ~ 2.0 mM
9	Sodium Phosphate			50 mM
10	dtt			1 mM
11	EDTA			1 mM
12	Azide			0.1 mM
13	H2O			90 %
14	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.101329

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.101329

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LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.101329

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LACS Plot; CO

Referencing offset: 0.17 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	TSP	methyl protons	0.0 ppm	external	indirect	0.101329

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Protein Blocks Logo

Calculated from 29 models in PDB: 2IF1, Strand ID: A Detail

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Release date

1999-11-22

Citation

Structure and interactions of the translation initiation factor eIF1
Fletcher, C.M., Pestova, T.V., Hellen, C.U.T., Wagner, G.
EMBO J. (1999), 4, 2631-2637, PubMed , DOI:

Related entities 1. Eukaryotic translation initiation factor 1, : 1 : 11 : 59 entities Detail

Interaction partners 1. Eukaryotic translation initiation factor 1, : 8 interactors Detail