BMRB: 4257

(52-96)C-Terminal Domain of the HIV-1 Regulatory Protein VPR

Authors

Schuler, W., de Rocquigny, H., Baudat, Y., Sire, J., Roques, B.P.

Assembly

Vpr HIV-1 (LAI)

Entity

1. Vpr HIV-1 (LAI) (polymer), 45 monomers, 5247.056 Da Detail

DTWTGVEALI RILQQLLFIH FRIGCRHSRI GIIQQRRTRN GASKS

Formula weight

5247.056 Da

Source organism

HIV-1 M:B_HXB2R

Exptl. method

NMR

Refine. method

DYNAMICAL SIMULATED ANNEALING, MOLECULAR DYNAMICS, ENERGY MINIMIZATION

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 96.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	97.9 % (283 of 289)	97.9 % (283 of 289)
Backbone	96.8 % (91 of 94)	96.8 % (91 of 94)
Sidechain	98.5 % (192 of 195)	98.5 % (192 of 195)
Aromatic	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Vpr HIV-1 (LAI)

DTWTGVEALI RILQQLLFIH FRIGCRHSRI GIIQQRRTRN GASKS

Show sample condition

Sample

Temperature 313 (±1) K, pH 3.4 (±0.1), Details low salt conditions

#	Name	Isotope labeling	Type	Concentration
1	Vpr_HIV-1_(LAI)			2.0 mM
2	DTT			10.0 mM
3	TFE	[U-2H]		4.1 M
4	D2O			5.6 M
5	H2O			33.3 M
6	TMSP			0.1 mM

Hide sample codition

Protein Blocks Logo

Calculated from 1 models in PDB: 1VPC, Strand ID: A Detail

---

Coupling constant

26 J values in 1 lists
Field strength (¹H) 600 MHz, Temperature 313 (±1) K, pH 3.4 (±0.1) Detail

---

Temperature 313 (±1) K, pH 3.4 (±0.1)

List #1 coupling_constants_set_one, Field strength (¹H) 600 MHz

Release date

1999-11-22

Citation

NMR structure of the (52-96) C-terminal domain of the HIV-1 regulatory protein Vpr: molecular insights into its biological functions
Schuler, W., Wecker, K., de Rocquigny, H., Baudat, Y., Sire, J., Roques, B.P.
J. Mol. Biol. (1999), 285, 2105-2117, PubMed 9925788 , DOI 10.1006/jmbi.1998.2381 , Abstract

Related entities 1. Vpr HIV-1 (LAI), : 1 : 4 : 44 entities Detail

Interaction partners 1. Vpr HIV-1 (LAI), : 7 interactors Detail

Experiments performed 3 experiments Detail