BMRB: 4261

Effects of Glycosylation on the Structure and Dynamics of Eel Calcitonin in Micelles and Lipid Bilayers Determined by NMR Spectroscopy

Authors

Hashimoto, Y., Toma, K., Nishikido, J., Yamamoto, K., Haneda, K., Inazu, T., Valentine, K., Opella, S.J.

Assembly

M6 eel calcitonin

Entity

1. M6 eel calcitonin (polymer, Thiol state: all disulfide bound), 32 monomers, 3417.865 Da Detail

CSNLSTCVLG KLSQELHKLQ TYPRTDVGAG TP

2. N-ACETYL-D-GLUCOSAMINE (non-polymer, Thiol state: not present), 221.208 Da

Total weight

3639.073 Da

Max. entity weight

3417.865 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS7:SG

Source organism

Anguilla

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.0 %, Completeness (bb): 96.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	94.0 % (171 of 182)	94.0 % (171 of 182)
Backbone	96.9 % (63 of 65)	96.9 % (63 of 65)
Sidechain	92.3 % (108 of 117)	92.3 % (108 of 117)
Aromatic	83.3 % (5 of 6)	83.3 % (5 of 6)
Methyl	94.7 % (18 of 19)	94.7 % (18 of 19)

1. M6 eel calcitonin

CSNLSTCVLG KLSQELHKLQ TYPRTDVGAG TP

Show sample condition

Sample

Temperature 308 (±0.1) K, Details Sodium dodecyl sulfate-d25 was used as micelles

#	Name	Isotope labeling	Type	Concentration
1	M6 eel calcitonin			1 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 1BZB, Strand ID: A Detail

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Release date

1999-12-02

Citation

Effects of glycosylation on the structure and dynamics of eel calcitonin in micelles and lipid bilayers determined by nuclear magnetic resonance spectroscopy
Hashimoto, Y., Toma, K., Nishikido, J., Yamamoto, K., Haneda, K., Inazu, T., Valentine, K.G., Opella, S.J.
Biochemistry (1999), 38, 8377-8384, PubMed 10387083 , DOI 10.1021/bi983018j , Abstract

Related entities 1. M6 eel calcitonin, : 1 : 3 : 2 : 40 entities Detail

Experiments performed 3 experiments Detail