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BMRB: 4263


4263
2NMB
dNumb PTB Domain Complexed with a Phosphotyrosine Peptide, NMR, Ensemble of Structures.
Authors
Zwahlen, C., Li, S.C., Vincent, S.J.F., Mcglade, C.J., Kay, L.E., Pawson, T., Forman-Kay, J.D.
Assembly
PTB domain of Drosophila Numb protein/GPpY peptide complex
Entity
1. PTB domain of Drosophila Numb protein (polymer), 160 monomers, 17883.13 × 2 Da Detail

GSPGIPDRVP ESSKPHQWQA DEEAVRSATC SFSVKYLGCV EVFESRGMQV CEEALKVLRQ SRRRPVRGLL HVSGDGLRVV DDETKGLIVD QTIEKVSFCA PDRNHERGFS YICRDGTTRR WMCHGFLACK DSGERLSHAV GCAFAVCLER KQRRTRAAAS


2. GPpY peptide (polymer), 7 monomers, 875.9013 × 2 Da Detail

AYIGPXL


Total weight
37518.062 Da
Max. entity weight
17883.13 Da
Source organism
Drosophila melanogaster
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING WITH AMBIGUOUS RESTRAINTS
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 95.2 %, Completeness: 88.7 %, Completeness (bb): 95.2 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All88.7 % (1660 of 1871)89.8 % (878 of 978)86.1 % (624 of 725)94.0 % (158 of 168)
Backbone95.2 % (935 of 982)95.0 % (322 of 339)95.0 % (460 of 484)96.2 % (153 of 159)
Sidechain83.8 % (872 of 1041)87.0 % (556 of 639)79.1 % (311 of 393)55.6 % (5 of 9)
Aromatic37.5 % (48 of 128)71.9 % (46 of 64) 0.0 % (0 of 62)100.0 % (2 of 2)
Methyl93.8 % (152 of 162)93.8 % (76 of 81)93.8 % (76 of 81)

1. PTB domain of Drosophila Numb protein

GSPGIPDRVP ESSKPHQWQA DEEAVRSATC SFSVKYLGCV EVFESRGMQV CEEALKVLRQ SRRRPVRGLL HVSGDGLRVV DDETKGLIVD QTIEKVSFCA PDRNHERGFS YICRDGTTRR WMCHGFLACK DSGERLSHAV GCAFAVCLER KQRRTRAAAS

2. GPpY peptide

AYIGPXL

Show sample condition
Sample

Pressure 1 bar, Temperature 303 (±0.5) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
1PTB domain of Drosophila Numb protein[U-100% 13C; U-100% 15N]1.2 mM
2GPpY peptide[99% 12C; 99% 14N]-pY, ALA [100% 13C, 100% 15N]-TYR, ILE, GLY, PRO, LEU1.2 mM

Chem. Shift Complete2
Sequence coverage: 34.1 %, Completeness: 57.7 %, Completeness (bb): 61.7 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All57.7 % (2158 of 3742)59.2 % (1158 of 1956)54.7 % (793 of 1450)61.6 % (207 of 336)
Backbone61.7 % (1212 of 1964)64.3 % (436 of 678)59.6 % (577 of 968)62.6 % (199 of 318)
Sidechain54.4 % (1132 of 2082)56.5 % (722 of 1278)51.1 % (402 of 786)44.4 % (8 of 18)
Aromatic29.3 % (75 of 256)46.9 % (60 of 128) 9.7 % (12 of 124)75.0 % (3 of 4)
Methyl59.0 % (191 of 324)60.5 % (98 of 162)57.4 % (93 of 162)

1. PTB domain of Drosophila Numb protein

GSPGIPDRVP ESSKPHQWQA DEEAVRSATC SFSVKYLGCV EVFESRGMQV CEEALKVLRQ SRRRPVRGLL HVSGDGLRVV DDETKGLIVD QTIEKVSFCA PDRNHERGFS YICRDGTTRR WMCHGFLACK DSGERLSHAV GCAFAVCLER KQRRTRAAAS

2. GPpY peptide

AYIGPXL

Show sample condition
Sample

Pressure 1 bar, Temperature 303 (±0.5) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
1PTB domain of Drosophila Numb protein[U-100% 13C; U-100% 15N]1.2 mM
2GPpY peptide[99% 12C; 99% 14N]-pY, ALA [100% 13C, 100% 15N]-TYR, ILE, GLY, PRO, LEU1.2 mM

Protein Blocks Logo
Calculated from 14 models in PDB: 2NMB, Strand ID: A, B Detail

Release date
1999-10-04
Citation 1
Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity
Zwahlen, C., Li, S.C., Vincent, S.J.F., Mcglade, C.J., Kay, L.E., Pawson, T., Forman-Kay, J.D.
Nat. Struct. Biol. (1998), 5, 1075-1083, PubMed 9846878 , DOI 10.1038/4185 ,
Show more 1 citaion
Citation 2
High-affinity binding of the Drosophila Numb phosphotyrosine-binding domain to peptides containing a Gly-Pro-(p)Tyr motif
Li, S.C., Songyang, Z., Vincent, S.J., Zwahlen, C., Wiley, S., Cantley, L., Kay, L.E., Forman-Kay, J., Pawson, T.
Proc. Natl. Acad. Sci. U. S. A. (1997), 94, 7204-7209, PubMed 9207069 , DOI: ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: P16554 released on 1990-08-01
    Title NUMB_DROME Entity Protein numb
Related entities 1. PTB domain of Drosophila Numb protein, : 52 entities Detail
More details for 52 related entities
Experiments performed 1 experiments Detail
nullKeywords Asymmetric Cell Division, Complex, Phosphotyrosine Binding Domain (PTB), Signal Transduction