BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1098 of 1203)	92.4 % (581 of 629)	89.4 % (414 of 463)	92.8 % (103 of 111)
Backbone	94.1 % (593 of 630)	93.6 % (206 of 220)	95.1 % (290 of 305)	92.4 % (97 of 105)
Sidechain	89.5 % (598 of 668)	91.9 % (376 of 409)	85.4 % (216 of 253)	100.0 % (6 of 6)
Aromatic	47.9 % (23 of 48)	87.5 % (21 of 24)	8.3 % (2 of 24)
Methyl	99.2 % (121 of 122)	98.4 % (60 of 61)	100.0 % (61 of 61)

1. resolvase

MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL LRMKVEEGDV ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD GEMGK

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1GHT, Strand ID: A Detail

Release date

1999-10-13

Citation

1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase
Pan, B., Mullen, G.P.
J. Biomol. NMR (1999), 13, 307-308, PubMed , DOI:

Related entities 1. gamma delta resolvase catalytic core, : 1 : 5 : 4 : 42 entities Detail

Experiments performed 9 experiments Detail

1 HNCA

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

2 HNCOCA

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

3 HNCACB

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

4 CBCACONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

5 HNCO,

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

6 CCONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

7 HCCONH

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

8 HCCH-TOCSY

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

9 1H-NOESY

Instrument

#1 Model Varian na (500 MHz)

#2 Model Varian na (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	resolvase	[U-15N]	2.0 mM
2	D2O		10 %
3	H2O		90 %

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	resolvase	[U-13C; U-15N]		2.0 mM

nullKeywords resolvase, recombination, DNA, Protein, NMR, Structure

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.09 seconds)

BMRB: 4269

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. gamma delta resolvase catalytic core, : 1 : 5 : 4 : 42 entities Detail

Experiments performed 9 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2