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Found 1 Document (1.152 seconds)

BMRB: 4271

1JFK

Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica

Authors

Govil, G., Chary, K.V.R., Sahu, S.C., Chauhan, S., Bhattacharya, A., Atreya, H.S.

Assembly

Calcium binding protein from Entamoeba Histolytica

Entity

1. Entamoeba Histolytica CaBP (polymer, Thiol state: not present), 134 monomers, 14952.69 Da Detail

MAEALFKEID VNGDGAVSYE EVKAFVSKKR AIKNEQLLQL IFKSIDADGN GEIDQNEFAK FYGSIQGQDL SDDKIGLKVL YKLMDVDGDG KLTKEEVTSF FKKHGIEKVA EQVMKADANG DGYITLEEFL EFSL

2. CA (non-polymer), 40.078 Da

Total weight

14992.769 Da

Max. entity weight

14952.69 Da

Source organism

Entamoeba histolytica

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 67.4 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.4 % (1064 of 1579)	64.1 % (526 of 821)	66.2 % (406 of 613)	91.0 % (132 of 145)
Backbone	97.1 % (781 of 804)	96.4 % (270 of 280)	97.4 % (380 of 390)	97.8 % (131 of 134)
Sidechain	44.8 % (402 of 897)	47.3 % (256 of 541)	42.0 % (145 of 345)	9.1 % (1 of 11)
Aromatic	0.0 % (0 of 126)	0.0 % (0 of 63)	0.0 % (0 of 63)
Methyl	21.9 % (32 of 146)	21.9 % (16 of 73)	21.9 % (16 of 73)

1. Entamoeba Histolytica CaBP

MAEALFKEID VNGDGAVSYE EVKAFVSKKR AIKNEQLLQL IFKSIDADGN GEIDQNEFAK FYGSIQGQDL SDDKIGLKVL YKLMDVDGDG KLTKEEVTSF FKKHGIEKVA EQVMKADANG DGYITLEEFL EFSL

Show sample condition

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵N	NH4Cl	nitrogen	24.93 ppm	external	direct	0.0
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵N	NH4Cl	nitrogen	24.93 ppm	external	direct	0.0
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0

Referencing offset: 0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵N	NH4Cl	nitrogen	24.93 ppm	external	direct	0.0
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0

Referencing offset: -0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹⁵N	NH4Cl	nitrogen	24.93 ppm	external	direct	0.0
¹³C	DSS	methyl carbons	0.0 ppm	external	direct	0.0

Referencing offset: -0.48 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JFJ, Strand ID: A Detail

Release date

2000-05-24

Citation

Sequence Specific 1H, 13C and 15N Assignments of a Calcium Binding Protein from Entamoeba Histolytica
Govil, G., Chary, K.V.R., Sahu, S.C., Chauhan, S., Bhattacharya, A., Atreya, H.S.
J. Biomol. NMR (1999), 14, 93-94, PubMed

Related entities 1. Entamoeba Histolytica CaBP, : 1 : 6 : 1 : 292 entities Detail

Experiments performed 8 experiments Detail

1 15N-HSQC

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

2 15N-TOCSY-HSQC

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

3 15N-NOESY-HSQC

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

4 HCA

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

5 H(CO)CA

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

6 HCO

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

7 CBCANH

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

8 CBCA(CO)NH

Instrument

Model Varian Unity plus (600 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Entamoeba Histolytica CaBP	[U-98% 13C; U-98% 15N]		3.0 ~ 4.0 mM

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Entamoeba Histolytica CaBP	[U-98% 15N]		3.0 ~ 4.0 mM

nullKeywords Calcium binding protein, EF family, NMR assignments, Entamoeba histolytica

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Found 1 Document (1.152 seconds)

BMRB: 4271

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Entamoeba Histolytica CaBP, : 1 : 6 : 1 : 292 entities Detail

Experiments performed 8 experiments Detail

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