BMRBj - BMREntryMaster

Found 1 Document (4.19 seconds)

BMRB: 4276

1B2I

Solution Structure and Dynamics of the Plasminogen Kringle 2-AMCHA Complex: 3/1-Helix in Homologous Domains

Authors

Marti, D.N., Schaller, J., Llinas, M.

Assembly

Plasminogen

Entity

1. Plasminogen (polymer, Thiol state: all disulfide bound), 83 monomers, 9636.687 Da Detail

TSEECMHGSG ENYDGKISKT MSGLECQAWD SQSPHAHGYI PSKFPNKNLK KNYCRNPDRE LRPWCFTTDP NKRWELCDIP RCT

2. AMH (non-polymer), 157.210 Da

Total weight

9793.896 Da

Max. entity weight

9636.687 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS82:SG
2	disulfide	sing	1:CYS26:SG	1:CYS65:SG
3	disulfide	sing	1:CYS54:SG	1:CYS77:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, DYNAMIC SIMULATED ANNEALING

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 69.7 %, Completeness (bb): 66.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.7 % (680 of 975)	97.5 % (505 of 518)	24.6 % (91 of 370)	96.6 % (84 of 87)
Backbone	66.3 % (321 of 484)	97.6 % (160 of 164)	36.1 % (88 of 244)	96.1 % (73 of 76)
Sidechain	63.3 % (360 of 569)	97.2 % (344 of 354)	2.5 % (5 of 204)	100.0 % (11 of 11)
Aromatic	53.3 % (49 of 92)	100.0 % (46 of 46)	0.0 % (0 of 43)	100.0 % (3 of 3)
Methyl	50.0 % (21 of 42)	100.0 % (21 of 21)	0.0 % (0 of 21)

1. Plasminogen

TSEECMHGSG ENYDGKISKT MSGLECQAWD SQSPHAHGYI PSKFPNKNLK KNYCRNPDRE LRPWCFTTDP NKRWELCDIP RCT

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1B2I, Strand ID: A Detail

Coupling constant

67 J values in 1 lists
Temperature 310 (±1) K, pH 5.1 (±0.1) Detail

Release date

1998-11-26

Citation

Solution structure and dynamics of the plasminogen kringle 2-AMCHA complex: 3(1)-helix in homologous domains
Marti, D.N., Schaller, J., Llinas, M.
Biochemistry (1999), 38, 15741-15755, PubMed 10625440 , DOI 10.1021/bi9917378 , Abstract

Related entities 1. Plasminogen, : 1 : 9 : 459 entities Detail

Interaction partners 1. Plasminogen, : 35 interactors Detail

More details for 35 interactors identified by 14 citations

Experiments performed 9 experiments Detail

1 COSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

2 NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

3 TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

4 3D-NOESY HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

5 3D-TOCSY HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

6 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

7 HN(CO)CA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

8 HCC(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

9 3D HNHA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Plasminogen			2.7 mM
2	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #2

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Plasminogen	[U-15N]		2.7 mM
4	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			8.1 mM

Sample #3

Temperature 310 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Plasminogen	[U-15N; U-13C]		1.2 mM
6	TRANS-4-AMINOMETHYLCYCLOHEXANE-1-CARBOXYLIC ACID			3.6 mM

nullKeywords FIBRINOLYSIS, KRINGLE 2, LYSINE-BINDING DOMAIN, PLASMINOGEN, SERINE PROTEASE

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Found 1 Document (4.19 seconds)

BMRB: 4276

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Related entities 1. Plasminogen, : 1 : 9 : 459 entities Detail

Interaction partners 1. Plasminogen, : 35 interactors Detail

Experiments performed 9 experiments Detail

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