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BMRB: 4378

2LCP

1H, 13C and 15N Resonance Assignment of un-myristoylated Ca2+-Frequenin, a Synaptic Efficacy Modulator

Authors

Kragelund, B.B., Hauenschild, A., Carlstrom, G., Pongs, O., Finn, B.E.

Assembly

calcium loaded un-myristoylated rat frequenin

Entity

1. calcium loaded un-myristoylated rat frequenin (polymer), 190 monomers, 21878.44 Da Detail

MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV

2. CA (non-polymer), 40.078 × 3 Da

Total weight

21998.674 Da

Max. entity weight

21878.44 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 80.4 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.4 % (1846 of 2297)	77.7 % (932 of 1200)	81.7 % (730 of 894)	90.6 % (184 of 203)
Backbone	96.3 % (1084 of 1126)	96.4 % (371 of 385)	96.2 % (537 of 558)	96.2 % (176 of 183)
Sidechain	69.2 % (933 of 1349)	68.8 % (561 of 815)	70.8 % (364 of 514)	40.0 % (8 of 20)
Aromatic	30.4 % (70 of 230)	36.5 % (42 of 115)	23.0 % (26 of 113)	100.0 % (2 of 2)
Methyl	94.1 % (175 of 186)	95.7 % (89 of 93)	92.5 % (86 of 93)

1. calcium loaded un-myristoylated rat frequenin

Show sample condition

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.72 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LCP, Strand ID: A Detail

Release date

2000-03-12

Citation

1H, 13C, and 15N assignments of un-myristoylated Ca2+-frequenin, a synaptic efficacy modulator
Kragelund, B.B., Hauenschild, A., Carlstrom, G., Pongs, O., Finn, B.E.
J. Biomol. NMR (2000), 16, 85-86, PubMed 10718617 , Abstract

Related entities 1. calcium loaded un-myristoylated rat frequenin, : 1 : 10 : 1 : 6 : 255 entities Detail

Interaction partners 1. calcium loaded un-myristoylated rat frequenin, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 3D 1H-15N NOESY-HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

2 3D 1H-15N TOCSY-HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

3 3D HNCA

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

4 3D HNCO

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

5 3D HNCOCA

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

6 3D CBCACOHN

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

7 3D CBCACOHA

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

8 3D HCCH-TOCSY

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

9 3D 1H-13C NOESY-HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

10 4D 1H-13C NOESY-HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

11 2D 1H-15N HSQC

Instrument

Model Varian UNITYplus (600 MHz)

Sample #1

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	calcium loaded un-myristoylated rat frequenin	[U-99% 15N]	1.4 mM
2	CaCl2		8 mM
3	DTT		14 mM
4	NaN3		0.2 %
5	D2O		10 %
6	H2O		90 %

Sample #2

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
8	CaCl2		8 mM
9	DTT		14 mM
10	NaN3		0.2 %
11	D2O		10 %
12	H2O		90 %

Sample #3

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
13	calcium loaded un-myristoylated rat frequenin	[U-99% 13C; U-99% 15N]	1.4 mM
14	CaCl2		8 mM
15	DTT		14 mM
16	NaN3		0.2 %
17	D2O		10 %
18	H2O		90 %

Sample #4

Temperature 300 (±1) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
19	calcium loaded un-myristoylated rat frequenin	[U-99% 13C]	1.4 mM
20	CaCl2		8 mM
21	DTT		14 mM
22	NaN3		0.2 %
23	D2O		100 %

nullKeywords calcium, EF-hand, frequenin, NMR, nuclear magnetic resonance

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Found 1 Document (2.904 seconds)

BMRB: 4378

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. calcium loaded un-myristoylated rat frequenin, : 1 : 10 : 1 : 6 : 255 entities Detail

Interaction partners 1. calcium loaded un-myristoylated rat frequenin, : 4 interactors Detail

Experiments performed 11 experiments Detail

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