BMRB: 4386

HIGH-RESOLUTION SOLUTION STRUCTURE OF FREE RGS4 BY NMR

Authors

Moy, F.J., Chanda, P.K., Cockett, M.I., Edris, W., Jones, P.G., Mason, K., Semus, S., Powers, R.

Assembly

REGULATOR OF G-PROTEIN SIGNALING 4

Entity

1. REGULATOR OF G-PROTEIN SIGNALING 4 (polymer, Thiol state: all free), 166 monomers, 19113.43 Da Detail

MRGSVSQEEV KKWAESLENL INHECGLAAF KAFLKSEYSE ENIDFWISCE EYKKIKSPSK LSPKAKKIYN EFISVQATKE VNLDSCTREE TSRNMLEPTI TCFDEAQKKI FNLMEKDSYR RFLKSRFYLD LTNPSSCGAE KQKGAKSSAD CTSLVPQCAH HHHHHP

Formula weight

19113.43 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 88.6 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.6 % (1756 of 1983)	89.7 % (936 of 1043)	86.9 % (665 of 765)	88.6 % (155 of 175)
Backbone	94.3 % (928 of 984)	95.5 % (315 of 330)	93.3 % (461 of 494)	95.0 % (152 of 160)
Sidechain	84.6 % (982 of 1161)	87.1 % (621 of 713)	82.7 % (358 of 433)	20.0 % (3 of 15)
Aromatic	60.5 % (104 of 172)	76.7 % (66 of 86)	42.9 % (36 of 84)	100.0 % (2 of 2)
Methyl	94.9 % (129 of 136)	92.6 % (63 of 68)	97.1 % (66 of 68)

1. REGULATOR OF G-PROTEIN SIGNALING 4

MRGSVSQEEV KKWAESLENL INHECGLAAF KAFLKSEYSE ENIDFWISCE EYKKIKSPSK LSPKAKKIYN EFISVQATKE VNLDSCTREE TSRNMLEPTI TCFDEAQKKI FNLMEKDSYR RFLKSRFYLD LTNPSSCGAE KQKGAKSSAD CTSLVPQCAH HHHHHP

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Sample

Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	REGULATOR OF G-PROTEIN SIGNALING 4	[U-15N; U-13C]		1 mM
2	phosphate buffer			50 mM
3	NaN3			2 mM
4	DTT	[U-2H]		5 mM
5	H2O			90 %
6	D2O			10 %

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Calculated from 30 models in PDB: 1EZY, Strand ID: A Detail

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Release date

2001-07-26

Citation

NMR Structure of Free RGS4 Reveals an Induced Conformational Change upon Binding G-alpha
Moy, F.J., Chanda, P.K., Cockett, M.I., Edris, W., Jones, P.G., Mason, K., Semus, S., Powers, R.
Biochemistry (2000), 39, 7063-7073, PubMed , DOI:

Related entities 1. REGULATOR OF G-PROTEIN SIGNALING 4, : 1 : 1 : 3 : 47 entities Detail

Interaction partners 1. REGULATOR OF G-PROTEIN SIGNALING 4, : 2 interactors Detail

Experiments performed 2 experiments Detail