BMRB: 4394

Translation initiation factor IF3 from Escherichia coli Ribosome binding domain (residues 84-180)

Authors

DE COCK, E., GARCIA, C., DARDEL, F.

Assembly

TRANSLATION INITIATION FACTOR IF3

Entity

1. TRANSLATION INITIATION FACTOR IF3 (polymer), 100 monomers, 11773.75 Da Detail

MEFQKKKQKV IQVKEIKFRP GTDEGDYQVK LRSLIRFLEE GDKAKITLRF RGREMAHQQI GMEVLNRVKD DLQELAVVES FPTKIEGRQM IMVLAPKKKQ

Formula weight

11773.75 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY AND RESTRAINED SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.0 %, Completeness: 72.0 %, Completeness (bb): 75.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	72.0 % (914 of 1269)	78.5 % (534 of 680)	59.8 % (288 of 482)	86.0 % (92 of 107)
Backbone	75.3 % (447 of 594)	90.1 % (183 of 203)	60.2 % (177 of 294)	89.7 % (87 of 97)
Sidechain	70.5 % (542 of 769)	73.6 % (351 of 477)	66.0 % (186 of 282)	50.0 % (5 of 10)
Aromatic	37.1 % (23 of 62)	74.2 % (23 of 31)	0.0 % (0 of 31)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. TRANSLATION INITIATION FACTOR IF3

MEFQKKKQKV IQVKEIKFRP GTDEGDYQVK LRSLIRFLEE GDKAKITLRF RGREMAHQQI GMEVLNRVKD DLQELAVVES FPTKIEGRQM IMVLAPKKKQ

Show sample condition

Sample #1

Pressure 1 atm, Temperature 303 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	TRANSLATION INITIATION FACTOR IF3	[U-98% 13C; U-95% 15N]		1.5 mM
2	potassium phosphate			20 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	TRANSLATION INITIATION FACTOR IF3	[U-98% 15N]		1.5 mM
4	potassium phosphate			20 mM

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LACS Plot; CA

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.25 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.28 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 24 models in PDB: 2IFE, Strand ID: A Detail

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Release date

1999-11-28

Citation

INTERACTION OF E. COLI TRANSLATION INITIATION FACTOR IF3 WITH THE RIBOSOME
DE COCK, E., BLANQUET, S., LALLEMAND, J.-Y., DARDEL, F.

Related entities 1. TRANSLATION INITIATION FACTOR IF3, : 1 : 7 : 120 entities Detail

Interaction partners 1. TRANSLATION INITIATION FACTOR IF3, : 15 interactors Detail

Experiments performed 5 experiments Detail