BMRB: 4395

RIBOSOMAL PROTEIN L25 FROM ESCHERICHIA COLI, NMR,

Authors

Stoldt, M., Wohnert, J., Gorlach, M., Brown, L.R.

Assembly

RIBOSOMAL PROTEIN L25

Entity

1. RIBOSOMAL PROTEIN L25 (polymer), 94 monomers, 10693.30 Da Detail

MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHID FVRA

Formula weight

10693.3 Da

Source organism

Escherichia coli

Exptl. method

NMR

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DISTANCE GEOMETRY/SIMULAT

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.9 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.9 % (1090 of 1137)	96.2 % (576 of 599)	95.0 % (418 of 440)	98.0 % (96 of 98)
Backbone	97.8 % (544 of 556)	98.9 % (187 of 189)	97.1 % (269 of 277)	97.8 % (88 of 90)
Sidechain	94.8 % (635 of 670)	94.9 % (389 of 410)	94.4 % (238 of 252)	100.0 % (8 of 8)
Aromatic	68.4 % (52 of 76)	68.4 % (26 of 38)	68.4 % (26 of 38)
Methyl	100.0 % (106 of 106)	100.0 % (53 of 53)	100.0 % (53 of 53)

1. RIBOSOMAL PROTEIN L25

MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHID FVRA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RIBOSOMAL PROTEIN L25	[U-13C; U-15N]		1.4 mM
2	KCl			100 mM

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LACS Plot; CA

Referencing offset: 1.42 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 1.42 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 1.9 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 1B75, Strand ID: A Detail

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Release date

2000-03-07

Citation

The NMR structure of Escherichia coli ribosomal protein L25 shows homology to general stress proteins and glutaminyl-tRNA synthetases
Stoldt, M., Wohnert, J., Gorlach, M., Brown, L.R.
EMBO J. (1998), 17, 6377-6384, PubMed 9799245 , DOI 10.1093/emboj/17.21.6377 , Abstract

Related entities 1. RIBOSOMAL PROTEIN L25, : 1 : 149 : 2 : 8 : 128 entities Detail

Experiments performed 1 experiments Detail