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BMRB: 4396

1COU

Anticoagulant protein from the nematode Ancylostoma caninum

Authors

Duggan, B.M., Dyson, H.JANE., Wright, P.E.

Assembly

Nematode Anticoagulant Protein c2

Entity

1. Nematode Anticoagulant Protein c2 (polymer, Thiol state: all disulfide bound), 85 monomers, 9745.618 Da Detail

KATMQCGENE KYDSCGSKEC DKKCKYDGVE EEDDEEPNVP CLVRVCHQDC VCEEGFYRNK DDKCVSAEDC ELDNMDFIYP GTRNP

Formula weight

9745.618 Da

Entity Connection

disulfide 5 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS50:SG
2	disulfide	sing	1:CYS15:SG	1:CYS46:SG
3	disulfide	sing	1:CYS20:SG	1:CYS41:SG
4	disulfide	sing	1:CYS24:SG	1:CYS70:SG
5	disulfide	sing	1:CYS52:SG	1:CYS64:SG

Source organism

Ancylostoma caninum

Exptl. method

NMR

Refine. method

MOLECULAR DYNAMICS

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.3 %, Completeness (bb): 56.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.3 % (607 of 944)	93.2 % (465 of 499)	16.0 % (57 of 357)	96.6 % (85 of 88)
Backbone	56.6 % (284 of 502)	97.7 % (167 of 171)	15.6 % (39 of 250)	96.3 % (78 of 81)
Sidechain	64.8 % (338 of 522)	90.9 % (298 of 328)	17.6 % (33 of 187)	100.0 % (7 of 7)
Aromatic	53.6 % (30 of 56)	100.0 % (28 of 28)	7.1 % (2 of 28)
Methyl	68.2 % (30 of 44)	100.0 % (22 of 22)	36.4 % (8 of 22)

1. Nematode Anticoagulant Protein c2

KATMQCGENE KYDSCGSKEC DKKCKYDGVE EEDDEEPNVP CLVRVCHQDC VCEEGFYRNK DDKCVSAEDC ELDNMDFIYP GTRNP

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.05), Details The 15N label is around 50-60%

#	Name	Isotope labeling	Concentration
1	Nematode Anticoagulant Protein c2	[U-55% 15N]	2.0 mM
2	sodium chloride		50 mM
3	DSS		20 uM

Protein Blocks Logo

Calculated from 18 models in PDB: 1COU, Strand ID: A Detail

Coupling constant

76 J values in 1 lists
Pressure 1 atm, Temperature 298 (±1) K, pH 4.5 (±0.05) Detail

Release date

2007-06-18

Citation 1

Inherent flexibility in a potent inhibitor of blood coagulation, recombinant nematode anticoagulant protein c2
Duggan, B.M., Dyson, H.JANE., Wright, P.E.
Eur. J. Biochem. (1999), 265, 539-548, PubMed 10504384 , DOI 10.1046/j.1432-1327.1999.00781.x , Abstract

Show more 3 citaions

Citation 2

Anticoagulant repertoire of the hookworm Ancylostoma caninum
Stassens, P., Bergum, P.W., Gansemans, Y., Jespers, L., Laroche, Y., Huang, S., Maki, S., Messens, J., Lauwereys, M., Cappello, M., Hotez, P.J., Lasters, I., Vlasuk, G.P.
Proc. Natl. Acad. Sci. U. S. A. (1996), 93, 2149-2154, PubMed 8700900 , DOI 10.1073/pnas.93.5.2149 , Abstract

Citation 3

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Citation 4

Stereochemical quality of protein structure coordinates
Morris, A.L., MacArthur, M.W., Hutchinson, E.G., Thornton, J.M.
Proteins (1992), 12, 345-364, PubMed 1579569 , DOI 10.1002/prot.340120407 , Abstract

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Related entities 1. Nematode Anticoagulant Protein c2, : 1 : 1 : 26 entities Detail

Experiments performed 9 experiments Detail

1 NOESY