BMRB: 4400

Structure and Mechanism of Formation of the H-y5 ismomer of an Intramolecular DNA Triple Helix.

Authors

van Dongen, M.J.P., Doreleijers, J.F., van der Marel, G.A., van Boom, J.H., Hilbers, C.W., Wijmenga, S.S.

Assembly

H-y5 Triple Helix

Entity

1. H-y5 Triple Helix (polymer), 30 monomers, 9135.792 Da Detail

TCTTCCTTTT CCTTCTCCCG AGAAGGTTTT

Formula weight

9135.792 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 72.5 %, Completeness (bb): 77.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	72.5 % (219 of 302)	72.5 % (219 of 302)
Suger, PO4	77.6 % (163 of 210)	77.6 % (163 of 210)
Nucleobase	60.9 % (56 of 92)	60.9 % (56 of 92)
Aromatic	70.0 % (42 of 60)	70.0 % (42 of 60)
Methyl	100.0 % (14 of 14)	100.0 % (14 of 14)

1. H-y5 Triple Helix

TCTTCCTTTT CCTTCTCCCG AGAAGGTTTT

Show sample condition

Sample

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	H-y5 Triple Helix			2.0 ~ 4.0 mM

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Release date

1999-10-25

Citation

Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix
van Dongen, M.J.P., Doreleijers, J.F., van der Marel, G.A., van Boom, J.H., Hilbers, C.W., Wijmenga, S.S.
Nat. Struct. Biol. (1999), 6, 854-859, PubMed 10467098 , DOI 10.1038/12313 , Abstract

Experiments performed 5 experiments Detail