BMRB: 4407

NMR chemical shift assignment of human GAIP (Galpha Interacting Protein): A regulator of G protein signaling

Authors

de Alba, E., De Vries, L., Farquhar, M.G., Tjandra, N.

Assembly

Gaip protein

Entity

1. Gaip protein (polymer), 153 monomers, 17322.19 Da Detail

GSPGISGGGG GIPSPEEVQS WAQSFDKLMH SPAGRSVFRA FLRTEYSEEN MLFWLACEEL KAEANQHVVD EKARLIYEDY VSILSPKEVS LDSRVREGIN KKMQEPSAHT FDDAQLQIYT LMHRDSYPRF LSSPTYRALL PWVDSSSSLI HRD

Formula weight

17322.19 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY, SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.6 %, Completeness: 78.6 %, Completeness (bb): 73.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.6 % (1397 of 1778)	84.1 % (780 of 927)	69.5 % (483 of 695)	85.9 % (134 of 156)
Backbone	73.4 % (661 of 900)	83.7 % (256 of 306)	62.4 % (281 of 450)	86.1 % (124 of 144)
Sidechain	84.6 % (865 of 1022)	84.4 % (524 of 621)	85.1 % (331 of 389)	83.3 % (10 of 12)
Aromatic	73.2 % (120 of 164)	75.6 % (62 of 82)	70.9 % (56 of 79)	66.7 % (2 of 3)
Methyl	88.9 % (128 of 144)	88.9 % (64 of 72)	88.9 % (64 of 72)

1. Gaip protein

GSPGISGGGG GIPSPEEVQS WAQSFDKLMH SPAGRSVFRA FLRTEYSEEN MLFWLACEEL KAEANQHVVD EKARLIYEDY VSILSPKEVS LDSRVREGIN KKMQEPSAHT FDDAQLQIYT LMHRDSYPRF LSSPTYRALL PWVDSSSSLI HRD

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 7.2 (±0.0)

#	Name	Isotope labeling	Type	Concentration
1	Gaip protein	[U-13C; U-15N]		0.6 ~ 1.0 mM

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LACS Plot; CA

Referencing offset: -0.35 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.35 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1CMZ, Strand ID: A Detail

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Release date

1999-11-10

Citation 1

Solution structure of human GAIP (G alpha interacting protein). A regulator of G protein signaling
de Alba, E., De Vries, L., Farquhar, M., Tjandra, N.
J. Mol. Biol. (1999), 291, 927-939, PubMed , DOI:

Show more 1 citaion

Citation 2

Solution structure of human GAIP (Galpha interacting protein): a regulator of G protein signaling
de Alba, E., De Vries, L., Farquhar, M.G., Tjandra, N.
J. Mol. Biol. (1999), 291, 927-939, PubMed 10452897 , DOI 10.1006/jmbi.1999.2989 , Abstract

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Gaip protein, : 1 : 2 : 223 entities Detail

Interaction partners 1. Gaip protein, : 5 interactors Detail

Experiments performed 8 experiments Detail