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BMRB: 4412


4412
1QL5
DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
Authors
Lee, J.-H., Choi, Y.-J., Choi, B.-S.
Assembly
DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3')
Entity
1. DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3') (polymer, Thiol state: not present), 10 monomers, 3071.984 Da Detail

CGCAXXACGC


2. strand- (polymer, Thiol state: not present), 10 monomers, 3138.986 Da Detail

GCGTTATGCG


Total weight
6210.9697 Da
Max. entity weight
3138.986 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 40.0 %, Completeness: 36.2 %, Completeness (bb): 31.7 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All36.2 % (64 of 177)36.2 % (64 of 177)
Suger, PO431.7 % (40 of 126)31.7 % (40 of 126)
Nucleobase47.1 % (24 of 51)47.1 % (24 of 51)
Aromatic44.4 % (16 of 36)44.4 % (16 of 36)
Methyl 0.0 % (0 of 3) 0.0 % (0 of 3)

1. DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3')

CGCAXXACGC

2. strand-

GCGTTATGCG

Show sample condition
Sample

Temperature 274.0 (±1) K, pH 6.6 (±0.1)


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3')1.0 mM
2strand-1.0 mM

Chem. Shift Complete2
Sequence coverage: 50.0 %, Completeness: 39.8 %, Completeness (bb): 35.7 % Detail
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Polymer type: polydeoxyribonucleotide

Total1H
All39.8 % (141 of 354)39.8 % (141 of 354)
Suger, PO435.7 % (90 of 252)35.7 % (90 of 252)
Nucleobase50.0 % (51 of 102)50.0 % (51 of 102)
Aromatic50.0 % (36 of 72)50.0 % (36 of 72)
Methyl50.0 % (3 of 6)50.0 % (3 of 6)

1. DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3')

CGCAXXACGC

2. strand-

GCGTTATGCG

Show sample condition
Sample

Temperature 274.0 (±1) K, pH 6.6 (±0.1)


#NameIsotope labelingTypeConcentration
1DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3')1.0 mM
2strand-1.0 mM

Release date
2000-06-18
Citation
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer
Lee, J.-H., Choi, Y.-J., Choi, B.-S.
Nucleic Acids Res. (2000), 28, 1794-1801, PubMed 10734199 ,
Experiments performed 1 experiments Detail
nullKeywords cis-syn dimer, DEOXYRIBONUCLEIC ACID, DNA PHOTOPRODUCT, MUTAGENESIS, NMR