BMRB: 4415

Solution-state structure of a DNA dodecamer duplex containing a cis-syn thymine cyclobutane dimer.

Authors

McAteer, K., Jing, Y., Kao, J., Taylor, J.-S., Kennedy, M.A.

Assembly

DNA dodecamer duplex

Entity

1. DNA (polymer, Thiol state: not present), 12 monomers, 4062.609 Da Detail

GCACGAATXA AG

2. DNA (polymer, Thiol state: not present), 12 monomers, 3691.349 Da Detail

CTTAATTCGT GC

Total weight

7753.958 Da

Max. entity weight

4062.609 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 45.8 %, Completeness: 36.6 %, Completeness (bb): 35.3 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	36.6 % (90 of 246)	35.9 % (80 of 223)	43.5 % (10 of 23)
Suger, PO4	35.3 % (65 of 184)	34.2 % (55 of 161)	43.5 % (10 of 23)
Nucleobase	40.3 % (25 of 62)	40.3 % (25 of 62)
Aromatic	43.5 % (20 of 46)	43.5 % (20 of 46)
Methyl	16.7 % (1 of 6)	16.7 % (1 of 6)

1. DNA

GCACGAATXA AG

2. DNA

CTTAATTCGT GC

Show sample condition

Sample

Temperature 278 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	DNA			0.7 mM
2	DNA			0.7 mM

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Chem. Shift Complete2

Sequence coverage: 50.0 %, Completeness: 39.0 %, Completeness (bb): 37.0 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	39.0 % (192 of 492)	38.3 % (171 of 446)	45.7 % (21 of 46)
Suger, PO4	37.0 % (136 of 368)	35.7 % (115 of 322)	45.7 % (21 of 46)
Nucleobase	45.2 % (56 of 124)	45.2 % (56 of 124)
Aromatic	47.8 % (44 of 92)	47.8 % (44 of 92)
Methyl	50.0 % (6 of 12)	50.0 % (6 of 12)

1. DNA

GCACGAATXA AG

2. DNA

CTTAATTCGT GC

Show sample condition

Sample

Temperature 278 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	DNA			0.7 mM
2	DNA			0.7 mM

Hide sample codition

Release date

1999-12-05

Citation

Solution-state structure of a DNA dodecamer duplex containing a Cis-syn thymine cyclobutane dimer, the major UV photoproduct of DNA
McAteer, K., Jing, Y., Kao, J., Taylor, J.-S., Kennedy, M.A.
J. Mol. Biol. (1998), 282, 1013-1032, PubMed 9753551 , DOI 10.1006/jmbi.1998.2062 , Abstract

Experiments performed 4 experiments Detail