BMRB: 4424

NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L9

Authors

Evans, S.P., Bycroft, M.

Assembly

ribonuclease HI

Entity

1. ribonuclease HI (polymer, Thiol state: all free), 63 monomers, 7010.517 Da Detail

GSARQGNFYA VRKGRETGIY NTWNECKNQV DGYGGAIYKK FNSYEQAKSF LGQPNTTSNY GSS

Formula weight

7010.517 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

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DISTANCE GEOMETRY AND SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 81.4 %, Completeness (bb): 83.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	81.4 % (589 of 724)	85.8 % (325 of 379)	74.9 % (203 of 271)	82.4 % (61 of 74)
Backbone	83.8 % (315 of 376)	97.8 % (131 of 134)	68.3 % (123 of 180)	98.4 % (61 of 62)
Sidechain	81.3 % (327 of 402)	79.2 % (194 of 245)	91.7 % (133 of 145)	0.0 % (0 of 12)
Aromatic	87.8 % (79 of 90)	88.9 % (40 of 45)	88.6 % (39 of 44)	0.0 % (0 of 1)
Methyl	100.0 % (36 of 36)	100.0 % (18 of 18)	100.0 % (18 of 18)

1. ribonuclease HI

GSARQGNFYA VRKGRETGIY NTWNECKNQV DGYGGAIYKK FNSYEQAKSF LGQPNTTSNY GSS

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Sample

Temperature 298 (±0.3) K, pH 3.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribonuclease HI	[U-13C; U-15N]		1.0 ~ 3.0 mM

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LACS Plot; CA

Referencing offset: 2.18 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.18 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1QHK, Strand ID: A Detail

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Release date

1999-12-22

Citation 1

NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L
Evans, S.P., Bycroft, M.
J. Mol. Biol. (1999), 291, 661-669, DOI:

Show more 2 citaions

Citation 2

The non-RNase H domain of Saccharomyces cerevisiae RNase H1 binds double-stranded RNA: magnesium modulates the switch between double-stranded RNA binding and RNase H activity
Cerritelli, S.M., Crouch, R.J.
RNA (1995), 1, 246-259, PubMed 7489497 , Abstract

Citation 3

Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant
Itaya, M., McKelvin, D., Chatterjie, S.K., Crouch, R.J.
Mol. Gen. Genet. (1991), 227, 438-445, PubMed 1650910 , DOI: , Abstract

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Entries sharing articles Swiss-Prot: 2 entries Detail

Related entities 1. ribonuclease HI, : 2 : 8 entities Detail

Interaction partners 1. ribonuclease HI, : 5 interactors Detail

Experiments performed 9 experiments Detail