BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	92.5 % (457 of 494)	91.5 % (389 of 425)	98.6 % (68 of 69)
Backbone	99.0 % (192 of 194)	99.2 % (131 of 132)	98.4 % (61 of 62)
Sidechain	88.3 % (265 of 300)	88.1 % (258 of 293)	100.0 % (7 of 7)
Aromatic	67.6 % (23 of 34)	66.7 % (22 of 33)	100.0 % (1 of 1)
Methyl	91.4 % (32 of 35)	91.4 % (32 of 35)

1. HEADPIECE DOMAIN FROM CHICKEN VILLIN

PTKLETFPLD VLVNTAAEDL PRGVDPSRKE NHLSDEDFKA VFGMTRSAFA NLPLWKQQNL KKEKGLF

Show sample condition

Sample

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 1QQV, Strand ID: A Detail

Coupling constant

42 J values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 293 K, pH 7.0 Detail

Release date

2001-07-29

Citation

NMR structure of an F-actin-binding "headpiece" motif from villin
Vardar, D., Buckley, D.A., Frank, B.S., McKnight, C.J.
J. Mol. Biol. (1999), 294, 1299-1310, PubMed 10600386 , DOI 10.1006/jmbi.1999.3321 , Abstract

Related entities 1. HEADPIECE DOMAIN FROM CHICKEN VILLIN, : 1 : 4 : 3 : 4 : 99 entities Detail

Interaction partners 1. HEADPIECE DOMAIN FROM CHICKEN VILLIN, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N]		1 mM
4	phosphate buffer			10 mM

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N; U-10% 13C]		1 mM
6	phosphate buffer			10 mM

Sample #4

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM

2 3D 15N-SEPARATED NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N]		1 mM
4	phosphate buffer			10 mM

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N; U-10% 13C]		1 mM
6	phosphate buffer			10 mM

Sample #4

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM

3 HNHA

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N]		1 mM
4	phosphate buffer			10 mM

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N; U-10% 13C]		1 mM
6	phosphate buffer			10 mM

Sample #4

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM

4 E-COSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N]		1 mM
4	phosphate buffer			10 mM

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N; U-10% 13C]		1 mM
6	phosphate buffer			10 mM

Sample #4

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM

5 HNHB

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM
2	phosphate buffer			10 mM

Sample #2

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
3	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N]		1 mM
4	phosphate buffer			10 mM

Sample #3

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	HEADPIECE DOMAIN FROM CHICKEN VILLIN	[U-15N; U-10% 13C]		1 mM
6	phosphate buffer			10 mM

Sample #4

Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	HEADPIECE DOMAIN FROM CHICKEN VILLIN			1 mM

nullKeywords F-actin binding domain, salt-bridge

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Sample

Related entities 1. HEADPIECE DOMAIN FROM CHICKEN VILLIN, : 1 : 4 : 3 : 4 : 99 entities Detail

Interaction partners 1. HEADPIECE DOMAIN FROM CHICKEN VILLIN, : 1 interactors Detail

Experiments performed 5 experiments Detail

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