BMRB: 4431

Structure of the Soluble Methane Monooxygenase Regulatory Protein B

Authors

Walters, K.J., Gassner, G.T., Lippard, S.J., Wagner, G.

Assembly

methane monooxygenase regulatory protein B

Entity

1. methane monooxygenase regulatory protein B (polymer, Thiol state: not reported), 141 monomers, 15983.72 Da Detail

MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT FIEEILLTDY KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF NVYDFLVDVS STIGRAYTLG NKFTITSELM GLDRKLEDYH A

Formula weight

15983.72 Da

Source organism

Methylococcus capsulatus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 67.7 %, Completeness (bb): 73.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	67.7 % (1112 of 1643)	77.7 % (656 of 844)	48.0 % (309 of 644)	94.8 % (147 of 155)
Backbone	73.8 % (623 of 844)	95.2 % (276 of 290)	51.4 % (213 of 414)	95.7 % (134 of 140)
Sidechain	60.3 % (561 of 931)	68.2 % (378 of 554)	47.0 % (170 of 362)	86.7 % (13 of 15)
Aromatic	18.0 % (27 of 150)	28.0 % (21 of 75)	5.5 % (4 of 73)	100.0 % (2 of 2)
Methyl	83.3 % (140 of 168)	90.5 % (76 of 84)	76.2 % (64 of 84)

1. methane monooxygenase regulatory protein B

MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT FIEEILLTDY KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF NVYDFLVDVS STIGRAYTLG NKFTITSELM GLDRKLEDYH A

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	methane monooxygenase regulatory protein B	[U-13C 100%; U-15N 100%]		1 mM
2	phosphate			50 mM

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LACS Plot; CA

Referencing offset: 7.66 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	TMS	methyl protons	0.0 ppm	internal	direct	0.0
15N	TSP	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; CB

Referencing offset: 7.66 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	TMS	methyl protons	0.0 ppm	internal	direct	0.0
15N	TSP	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	0.0
13C	TMS	methyl protons	0.0 ppm	internal	direct	0.0
15N	TSP	methyl protons	0.0 ppm	internal	direct	0.0

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Protein Blocks Logo

Calculated from 14 models in PDB: 1CKV, Strand ID: A Detail

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Release date

2000-05-10

Citation

Structure of the soluble methane monooxygenase regulatory protein B
Walters, K.J., Gassner, G.T., Lippard, S.J., Wagner, G.
Proc. Natl. Acad. Sci. U. S. A. (1999), 96, 7877-7882, PubMed 10393915 , DOI 10.2210/pdb1ckv/pdb , Abstract

Related entities 1. methane monooxygenase regulatory protein B, : 1 : 2 : 16 entities Detail

Interaction partners 1. methane monooxygenase regulatory protein B, : 1 interactors Detail

Experiments performed 10 experiments Detail