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Found 1 Document (1.832 seconds)

BMRB: 4437

1CEJ

Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1

Authors

MORGAN, W.D., BIRDSALL, B., FRENKIEL, T.A., GRADWELL, M.G., BURGHAUS, P.A., SYED, S.E.H., UTHAIPIBULL, C., HOLDER, A.A., FEENEY, J.

Assembly

MEROZOITE SURFACE PROTEIN 1

Entity

1. MEROZOITE SURFACE PROTEIN 1 (polymer, Thiol state: all disulfide bound), 96 monomers, 10631.59 Da Detail

NISQHQCVKK QCPQNSGCFR HLDEREECKC LLNYKQEGDK CVENPNPTCN ENNGGCDADA KCTEEDSGSN GKKITCECTK PDSYPLFDGI FCSSSN

Formula weight

10631.59 Da

Entity Connection

disulfide 6 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS18:SG
2	disulfide	sing	1:CYS12:SG	1:CYS28:SG
3	disulfide	sing	1:CYS30:SG	1:CYS41:SG
4	disulfide	sing	1:CYS49:SG	1:CYS62:SG
5	disulfide	sing	1:CYS56:SG	1:CYS76:SG
6	disulfide	sing	1:CYS78:SG	1:CYS92:SG

Source organism

Plasmodium falciparum

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.1 %, Completeness (bb): 95.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (987 of 1060)	96.1 % (540 of 562)	88.3 % (346 of 392)	95.3 % (101 of 106)
Backbone	95.1 % (538 of 566)	96.4 % (187 of 194)	93.2 % (262 of 281)	97.8 % (89 of 91)
Sidechain	92.1 % (537 of 583)	95.9 % (353 of 368)	86.0 % (172 of 200)	80.0 % (12 of 15)
Aromatic	59.3 % (32 of 54)	100.0 % (27 of 27)	18.5 % (5 of 27)
Methyl	100.0 % (48 of 48)	100.0 % (24 of 24)	100.0 % (24 of 24)

1. MEROZOITE SURFACE PROTEIN 1

NISQHQCVKK QCPQNSGCFR HLDEREECKC LLNYKQEGDK CVENPNPTCN ENNGGCDADA KCTEEDSGSN GKKITCECTK PDSYPLFDGI FCSSSN

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	MEROZOITE SURFACE PROTEIN 1			2.1 ~ 2.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.25 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.25 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.6 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 32 models in PDB: 1CEJ, Strand ID: A Detail

Release date

1999-12-21

Citation

Solution structure of an EGF module pair from the Plasmodium falciparum merozoite surface protein 1
MORGAN, W.D., BIRDSALL, B., FRENKIEL, T.A., GRADWELL, M.G., BURGHAUS, P.A., SYED, S.E.H., UTHAIPIBULL, C., HOLDER, A.A., FEENEY, J.
J. Mol. Biol. (1999), 289, 113-122, PubMed 10339410 , DOI 10.1006/jmbi.1999.2753 , Abstract

Related entities 1. MEROZOITE SURFACE PROTEIN 1, : 1 : 3 : 2 : 2 : 42 entities Detail

Experiments performed 22 experiments Detail

1 2D-NOESY