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BMRB: 4445

1D8B

1H, 13C, and 15N Chemical Shift Assignments and coupling constants for the HRDC domain from S. cerevisiae Sgs1 RecQ helicase

Authors

Liu, Z., Macias, M.J., Bottomley, M.J., Stier, G., Linge, J.P., Nilges, M., Bork, P., Sattler, M.

Assembly

HRDC domain

Entity

1. HRDC domain (polymer, Thiol state: not present), 91 monomers, 10681.35 Da Detail

MKHHHHHHPM ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS E

Formula weight

10681.35 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

ARIA (AMBIGUOUS RESTRAINTS IN ITERATIVE ASSIGNMENTS) AMBIGUOUS DISTANCE RESTRAINTS SIMULATED ANNEALING WITH TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete1

Sequence coverage: 91.2 %, Completeness: 77.7 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	77.7 % (863 of 1111)	81.8 % (480 of 587)	69.2 % (299 of 432)	91.3 % (84 of 92)
Backbone	77.4 % (415 of 536)	91.1 % (164 of 180)	64.1 % (173 of 270)	90.7 % (78 of 86)
Sidechain	79.6 % (528 of 663)	77.6 % (316 of 407)	82.4 % (206 of 250)	100.0 % (6 of 6)
Aromatic	40.9 % (36 of 88)	43.2 % (19 of 44)	38.6 % (17 of 44)
Methyl	98.8 % (83 of 84)	100.0 % (42 of 42)	97.6 % (41 of 42)

1. HRDC domain of Sgs1 RecQ helicase

MKHHHHHHPM ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS E

Show sample condition

Sample

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Chem. Shift Complete2

Sequence coverage: 91.2 %, Completeness: 62.9 %, Completeness (bb): 62.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.9 % (1398 of 2222)	77.3 % (907 of 1174)	38.7 % (334 of 864)	85.3 % (157 of 184)
Backbone	62.1 % (666 of 1072)	88.6 % (319 of 360)	36.3 % (196 of 540)	87.8 % (151 of 172)
Sidechain	61.8 % (819 of 1326)	72.2 % (588 of 814)	45.0 % (225 of 500)	50.0 % (6 of 12)
Aromatic	34.7 % (61 of 176)	45.5 % (40 of 88)	23.9 % (21 of 88)
Methyl	76.2 % (128 of 168)	98.8 % (83 of 84)	53.6 % (45 of 84)

1. HRDC domain of Sgs1 RecQ helicase

MKHHHHHHPM ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS E

Show sample condition

Sample

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Protein Blocks Logo

Calculated from 15 models in PDB: 1D8B, Strand ID: A Detail

Coupling constant

53 J values in 1 lists
Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2) Detail

Release date

2000-07-17

Citation 1

The 3D structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins
Stier, G., Linge, J.P., Nilges, M., Bork, P., Sattler, M., Macias, M.J., Bottomley, M.J., Liu, Z.
Structure (1999), 7, 1557-1566, PubMed , DOI:

Show more 5 citaions

Citation 2

Ambiguous NOEs and automated NOE assignment
Nilges, M., O'Donoghue, S.I.
Prog. NMR Spectroscopy (1998), 32, 107-139, PubMed , DOI 10.1016/S0079-6565(97)00025-3

Citation 3

Crystallography & NMR system: A new software suite for macromolecular structure determination
Pannu, N.S., Read, R.J., Rice, L.M., Warren, G.L., Simonson, T., Brunger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Kuszewski, J., Nilges, M.
Acta Crystallogr. D. Biol. Crystallogr. (1998), 54, 905-921, PubMed 9757107 , DOI: , Abstract

Citation 4

Refinement of the protein backbone angle psi in NMR structure calculations
Nilges, M., Sprangers, R., Bottomley, M., Linge, J., Schultz, J., Sattler, M.
J. Biomol. NMR (2000), 16, 47-58, PubMed 10718612 , DOI: , Abstract

Citation 5

Influence of non-bonded parameters on the quality of NMR structures: a new force field for NMR structure calculation
Linge, J., Nilges, M.
J. Biomol. NMR (1999), 13, 51-59, PubMed 10905826 , DOI: , Abstract

Citation 6

Automated assignment of ambiguous nuclear overhauser effects with ARIA
O'Donoghue, S.I., Linge, J.P., Nilges, M.
Methods. Enzymol. (2001), 339, 71-90, PubMed 11462826 , DOI: , Abstract

Hide non-primary 5 citaions

Related entities 1. HRDC domain, : 1 : 2 entities Detail

Interaction partners 1. HRDC domain, : 18 interactors Detail

More details for 18 interactors identified by 11 citations

Experiments performed 16 experiments Detail

1 3D HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

2 3D CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

3 3D CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

4 3D HCCONH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

5 3D CCONH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

6 3D HCCH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

7 3D proline-edited CDCA(NCO)CAHA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

8 1H-15N-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

9 1H-13C-HMQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

10 3D HNHA-J

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

11 2D HNCG

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

12 2D aromatic (HB)CB(CGCD/CE)HD/HE

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

13 The structures have been directly refined against cross-correlated relaxation

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

14 rates (C-alpha-H-alpha dipole, CO CSA) and the three-bond H/D isotope effect on

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

15 the C-alpha chemical shift. Pulse sequences and experimental details are

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

16 described in reference 3.

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
1	HRDC domain of Sgs1 RecQ helicase	[U-15N]	1.5 ~ 1.8 mM
2	sodium phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
5	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.5 ~ 1.8 mM
6	sodium phosphate		20 mM
7	D2O		100 %

Sample #3

Pressure 1 atm, Temperature 295 (±0.3) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
8	HRDC domain of Sgs1 RecQ helicase	[U-13C; U-15N]	1.3 mM
9	sodium phosphate		20 mM
10	sodium chloride		50 mM
11	sodium azide		0.02 %
12	D2O		50 %
13	H2O		50 %

nullKeywords Bloom syndrome, Werner syndrome, HRDC domain, RecQ helicases

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Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.749 seconds)

BMRB: 4445

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Related entities 1. HRDC domain, : 1 : 2 entities Detail

Interaction partners 1. HRDC domain, : 18 interactors Detail

Experiments performed 16 experiments Detail

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